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2.
Curr HIV Res ; 12(1): 32-43, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-24813637

RESUMO

Through the use of highly active antiretroviral therapy a significant reduction occurred in mortality and morbidity caused by Human Immunodeficiency Virus. The use of antiretroviral drugs resulted in the emergence of resistant viral strains due to mutations that cause a selective advantage to the virus. The aim of our study is to monitor the HIV-1 infection in Sicilians patients evaluating the presence of mutations that make the virus resistant to the therapy. The QIAGEN QIAamp Viral RNA Mini Kit was used to extract HIV-1 viral RNA from 300 patients while the TRUGENE HIV-1 Genotyping Kit and the OpenGene DNA Sequencing System determined viral mutations in the RNA samples. The analysis showed that from 300 subjects, 116 developed Antiretroviral Drug Resistance. The percentage of patients with resistance to nucleoside reverse transcriptase inhibitor (NRTI), non nucleoside reverse transcriptase inhibitor (NNRTI) and protease inhibitor was 26%, 23% and 20%, respectively. Comparison between drug resistances and mutations showed that 134 individuals had mutations in genes codifying for reverse transcriptase but a little more than 50% were associated with resistance to reverse transcriptase inhibitors, in particular 78 and 68 subjects developed drug resistances to NRTI and NNRTI classes respectively. Subjects that showed mutations in genes codifying for protease were 216 but only 59 of these were associated with resistance to protease inhibitors. Our findings emphasize the importance of continued resistance surveillance. Monitoring of transmitted resistance continues to be needed among treatment-exposed patients because of the benefit it provides for the development of drugs effective against the most frequently found drug-resistant viruses.


Assuntos
Antirretrovirais/uso terapêutico , Farmacorresistência Viral/genética , Infecções por HIV/tratamento farmacológico , HIV-1/genética , Adulto , Feminino , Infecções por HIV/epidemiologia , Infecções por HIV/virologia , Inibidores da Protease de HIV/uso terapêutico , HIV-1/efeitos dos fármacos , Humanos , Masculino , Pessoa de Meia-Idade , Prevalência , RNA Viral/análise , Inibidores da Transcriptase Reversa/uso terapêutico , Análise de Sequência de DNA , Sicília/epidemiologia
3.
AIDS Res Hum Retroviruses ; 28(9): 1110-8, 2012 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-22050664

RESUMO

Regressive morphological lesions, found in peripheral lymphocytes from HIV(+) patients, clearly conflict with normal cycle progression and with the execution of basic housekeeping and immune functions. With these lesions, circulating lymphocytes are destined to spontaneous and energy-independent cell lysis. By means of confocal microscopy and morphometry, we have quantified the rate of circulating T cells that are probably destined to emocatheresis in vivo. This rate includes lymphocytes in which nucleolin fragments have been scattered out of the nuclear region as a result of prelethal alterations in the nuclear membrane permeability. In terms of bioenergetics, these cells show evident anomalies in the energy production machinery that make them unable to carry out ATP-requiring functions. The extent of damaged cell fraction in peripheral blood reflects the frequency with which T lymphocytes leave lymphoid tissue to be cleared in hemocatheretic processes.


Assuntos
Síndrome da Imunodeficiência Adquirida/imunologia , Linfócitos T CD4-Positivos/imunologia , Linfócitos T CD8-Positivos/imunologia , Soropositividade para HIV/imunologia , HIV-1/imunologia , Ativação Linfocitária , Fosfoproteínas/metabolismo , Proteínas de Ligação a RNA/metabolismo , Carga Viral/imunologia , Adulto , Apoptose , Western Blotting , Feminino , Citometria de Fluxo , Humanos , Imuno-Histoquímica , Masculino , Microscopia Confocal , Nucleolina
4.
J Clin Microbiol ; 48(10): 3806-9, 2010 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-20702671

RESUMO

Neurological involvement of the central nervous system in brucellosis is uncommon. We describe a rare case of meningoencephalitis due to Brucella melitensis infection, associated with the syndrome of inappropriate antidiuretic hormone secretion and leading to diabetes insipidus and hypothyroidism. Neurobrucellosis, although rare, should be considered in cases of neurological disease of unknown etiology.


Assuntos
Brucella melitensis/isolamento & purificação , Brucelose/complicações , Brucelose/diagnóstico , Diabetes Insípido/diagnóstico , Hipotireoidismo/diagnóstico , Meningoencefalite/complicações , Meningoencefalite/diagnóstico , Adolescente , Brucelose/microbiologia , Diabetes Insípido/etiologia , Humanos , Hipotireoidismo/etiologia , Masculino , Meningoencefalite/microbiologia , Vasopressinas/metabolismo
6.
Cell Cycle ; 9(11): 2130-40, 2010 Jun 01.
Artigo em Inglês | MEDLINE | ID: mdl-20505329

RESUMO

The HIV-induced demise of CD4-T cells is thought to be a result of the execution of genetically programmed cell death that occurs in lymphoid tissue, where many resident T cells are chronically hyperactivated. Since HIV-induced alterations of cell cycle control has been often indicated as prominent mechanism of immune hyper activation and cause of apoptotic death, the signal pathway involved in cell cycle dysregulation of T lymphocytes from HIV infected patients was extensively studied. Here, we also demonstrate that circulating T lymphocytes leave lymphoid tissues with diffused regressive lesions (vacuolization, blebbing, nuclear evanescence and organelle swelling). Equally diffused are biochemical anomalies that accompany the overall disarrangement of cell structure, particularly the fragmentation and diffusion into the cytoplasm of C23/nucleolin, the intracellular accumulation of short lived regulatory proteins and the decrease in expression of membrane proteins. All this is something more than a cell cycle-related remodelling of cell morphology and biochemical mechanisms, and rather recalls a necrotic/oncotic cell damage. Since these changes are associated with adaptive mechanisms to hypoxia, we give evidence for alteration of cell cycle control developing in conditions of scarce energy supply.


Assuntos
Proteínas de Ciclo Celular/metabolismo , Infecções por HIV/metabolismo , Fosfoproteínas/metabolismo , Proteínas de Ligação a RNA/metabolismo , Linfócitos T/ultraestrutura , Apoptose , Ciclo Celular , Ciclina B1/metabolismo , Ciclina D/metabolismo , HIV , Infecções por HIV/imunologia , Humanos , Fator 1 Induzível por Hipóxia/metabolismo , Fosfoproteínas/análise , Proteínas de Ligação a RNA/análise , Linfócitos T/imunologia , Linfócitos T/metabolismo , Ubiquitinação , Nucleolina
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