Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 6 de 6
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
1.
PLoS One ; 7(5): e37355, 2012.
Artigo em Inglês | MEDLINE | ID: mdl-22649521

RESUMO

Inteins are intervening protein domains with self-splicing ability that can be used as molecular switches to control activity of their host protein. Successfully engineering an intein into a host protein requires identifying an insertion site that permits intein insertion and splicing while allowing for proper folding of the mature protein post-splicing. By analyzing sequence and structure based properties of native intein insertion sites we have identified four features that showed significant correlation with the location of the intein insertion sites, and therefore may be useful in predicting insertion sites in other proteins that provide native-like intein function. Three of these properties, the distance to the active site and dimer interface site, the SVM score of the splice site cassette, and the sequence conservation of the site showed statistically significant correlation and strong predictive power, with area under the curve (AUC) values of 0.79, 0.76, and 0.73 respectively, while the distance to secondary structure/loop junction showed significance but with less predictive power (AUC of 0.54). In a case study of 20 insertion sites in the XynB xylanase, two features of native insertion sites showed correlation with the splice sites and demonstrated predictive value in selecting non-native splice sites. Structural modeling of intein insertions at two sites highlighted the role that the insertion site location could play on the ability of the intein to modulate activity of the host protein. These findings can be used to enrich the selection of insertion sites capable of supporting intein splicing and hosting an intein switch.


Assuntos
Elementos de DNA Transponíveis/genética , Inteínas/genética , Modelos Moleculares , Engenharia de Proteínas/métodos , Proteínas/química , Proteínas/genética , Área Sob a Curva , Western Blotting , Endo-1,4-beta-Xilanases/genética , Conformação Proteica , Processamento de Proteína , Curva ROC , beta-Glucosidase/genética
2.
Mol Microbiol ; 66(6): 1331-41, 2007 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-17986190

RESUMO

Virstatin is a previously described small molecule inhibitor of Vibrio cholerae virulence. We have demonstrated that the molecule inhibits the activity of the transcriptional activator ToxT, thereby preventing elaboration of the toxin co-regulated pilus (TCP) and cholera toxin in vitro and in vivo in O1 strains of V. cholerae. While strains of the O1 and O139 serogroups are the cause of most epidemic and endemic cholera currently seen globally, sporadic disease caused by strains of non-O1/non-O139 serogroups suggests that understanding the pathogenic mechanisms of these unusual strains is relevant for disease. Although some non-O1/non-O139 strains have acquired the pathogenicity island that encodes the TCP, the role that this essential colonization factor of O1/O139 strains plays in the virulence of non-O1/non-O139 strains has not been determined. In this study, we utilize virstatin in a 'chemical genetic approach' to examine the role of ToxT, and thus by inference TCP, in the colonization of a panel of predominantly non-O1/non-O139 tcp+ strains. We identified nine strains whose colonization was resistant to virstatin inhibition in the infant mouse model. These strains presumably colonize by a TCP-independent mechanism or contain a naturally occurring virstatin-resistant ToxT. Four strains contained the typical toxT gene found in O1/O139 strains (toxT(EPI)) isolated from cholera epidemics. Interruption of toxT in one of these strains did not affect colonization of the infant mouse small intestine. The remaining five strains were found to contain a sequence divergent toxT gene that has been previously designated toxT(ENV) because of its occurrence in isolates of V. cholerae from the environment. We show that ToxT(ENV) is resistant to virstatin in two separate heterologous systems and is necessary for efficient colonization of the infant mouse small intestine. These results support the new concept that chemical genetic probes for the in vivo function or expression of virulence genes can be used to identify strains that express alternative virulence factors or novel regulatory systems that are functional in vivo.


Assuntos
Butiratos/farmacologia , Farmacorresistência Bacteriana/genética , Naftalenos/farmacologia , Naftalimidas/farmacologia , Vibrio cholerae/efeitos dos fármacos , Vibrio cholerae/genética , Sequência de Aminoácidos , Animais , Proteínas de Bactérias/genética , Proteínas de Bactérias/metabolismo , Western Blotting , Genoma Bacteriano , Intestino Delgado/microbiologia , Camundongos , Dados de Sequência Molecular , Regiões Promotoras Genéticas , Homologia de Sequência de Aminoácidos , Fatores de Transcrição/genética , Fatores de Transcrição/metabolismo , Vibrio cholerae/patogenicidade , Virulência/genética
3.
Proc Natl Acad Sci U S A ; 104(39): 15508-13, 2007 Sep 25.
Artigo em Inglês | MEDLINE | ID: mdl-17873062

RESUMO

Genes encoding type VI secretion systems (T6SS) are widely distributed in pathogenic Gram-negative bacterial species. In Vibrio cholerae, T6SS have been found to secrete three related proteins extracellularly, VgrG-1, VgrG-2, and VgrG-3. VgrG-1 can covalently cross-link actin in vitro, and this activity was used to demonstrate that V. cholerae can translocate VgrG-1 into macrophages by a T6SS-dependent mechanism. Protein structure search algorithms predict that VgrG-related proteins likely assemble into a trimeric complex that is analogous to that formed by the two trimeric proteins gp27 and gp5 that make up the baseplate "tail spike" of Escherichia coli bacteriophage T4. VgrG-1 was shown to interact with itself, VgrG-2, and VgrG-3, suggesting that such a complex does form. Because the phage tail spike protein complex acts as a membrane-penetrating structure as well as a conduit for the passage of DNA into phage-infected cells, we propose that the VgrG components of the T6SS apparatus may assemble a "cell-puncturing device" analogous to phage tail spikes to deliver effector protein domains through membranes of target host cells.


Assuntos
Actinas/metabolismo , Actinas/química , Trifosfato de Adenosina/química , Animais , Bacteriófagos/metabolismo , Transporte Biológico , Reagentes de Ligações Cruzadas/química , DNA/metabolismo , DNA Polimerase Dirigida por DNA/metabolismo , Dictyostelium/metabolismo , Escherichia coli/metabolismo , Plasmídeos/metabolismo , Conformação Proteica , Estrutura Terciária de Proteína
4.
Proc Natl Acad Sci U S A ; 103(5): 1528-33, 2006 Jan 31.
Artigo em Inglês | MEDLINE | ID: mdl-16432199

RESUMO

The bacterium Vibrio cholerae, like other human pathogens that reside in environmental reservoirs, survives predation by unicellular eukaryotes. Strains of the O1 and O139 serogroups cause cholera, whereas non-O1/non-O139 strains cause human infections through poorly defined mechanisms. Using Dictyostelium discoideum as a model host, we have identified a virulence mechanism in a non-O1/non-O139 V. cholerae strain that involves extracellular translocation of proteins that lack N-terminal hydrophobic leader sequences. Accordingly, we have named these genes "VAS" genes for virulence-associated secretion, and we propose that these genes encode a prototypic "type VI" secretion system. We show that vas genes are required for cytotoxicity of V. cholerae cells toward Dictyostelium amoebae and mammalian J774 macrophages by a contact-dependent mechanism. A large number of Gram-negative bacterial pathogens carry genes homologous to vas genes and potential effector proteins secreted by this pathway (i.e., hemolysin-coregulated protein and VgrG). Mutations in vas homologs in other bacterial species have been reported to attenuate virulence in animals and cultured macrophages. Thus, the genes encoding the VAS-related, type VI secretion system likely play an important conserved function in microbial pathogenesis and represent an additional class of targets for vaccine and antimicrobial drug-based therapies.


Assuntos
Dictyostelium/metabolismo , Regulação Bacteriana da Expressão Gênica , Vibrio cholerae/metabolismo , Animais , Proteínas de Bactérias/química , Linhagem Celular , Vacinas contra Cólera , Elementos de DNA Transponíveis , Eletroforese em Gel de Poliacrilamida , Biblioteca Gênica , Genes Bacterianos , Macrófagos/metabolismo , Camundongos , Modelos Biológicos , Modelos Genéticos , Transporte Proteico , Fatores de Tempo , Virulência
5.
Mol Microbiol ; 59(1): 193-201, 2006 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-16359328

RESUMO

Vibrio cholerae is a Gram-negative bacterium that causes the acute diarrhoeal disease cholera. After the bacterium is ingested, it passes through the digestive tract, encountering various environmental stresses including the acidic milieu of the stomach and the toxic effects of bile in the duodenum. While these stresses serve as part of a host defence system, V. cholerae has evolved resistance mechanisms that allow it to evade these defences and establish infection. We examined the expression profiles of V. cholerae in response to bile or bile acids and found an induction of biofilm genes. We found that V. cholerae shows significantly enhanced biofilm formation in response to bile acids, and that bacteria within the biofilm are more resistant to the toxicity of bile acids compared with planktonic cells. Bile acid induction of biofilms was found to be dependent on the vps genes (Vibrio polysaccharide synthesis) and their transcriptional activator VpsR, but VpsT is not required. These results contribute to the developing picture of a complex relationship between V. cholerae and its environment within the host during infection.


Assuntos
Ácidos e Sais Biliares/metabolismo , Biofilmes , Regulação Bacteriana da Expressão Gênica , Vibrio cholerae/metabolismo , Proteínas de Bactérias/genética , Proteínas de Bactérias/metabolismo , Genes Reporter , Humanos , Polissacarídeos Bacterianos/metabolismo , Vibrio cholerae/genética
6.
Proc Natl Acad Sci U S A ; 102(9): 3465-70, 2005 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-15728357

RESUMO

Non-O1, non-O139 Vibrio cholerae can cause gastroenteritis and extraintestinal infections, but, unlike O1 and O139 strains of V. cholerae, little is known about the virulence gene content of non-O1, non-O139 strains and their phylogenetic relationship to other pathogenic V. cholerae. Comparative genomic microarray analysis of four pathogenic non-O1, non-O139 strains indicates that these strains are quite divergent from O1 and O139 strains. Genomic sequence analysis of a non-O1, non-O139 strain (AM-19226) that appeared particularly pathogenic in experimental animals suggests that this strain carries a type III secretion system (TTSS) that is related to the TTSS2 gene cluster found in a pandemic clone of Vibrio parahaemolyticus. The genes for this V. cholerae TTSS system appear to be present in many clinical and environmental non-O1, non-O139 strains, including at least one clone that is globally distributed. We hypothesize that the TTSS present in some pathogenic strains of non-O1, non-O139 V. cholerae may be involved in the virulence and environmental fitness of these strains.


Assuntos
Genes Bacterianos , Genoma Bacteriano , Vibrio cholerae/genética , Animais , Southern Blotting , Diarreia/microbiologia , Modelos Animais , Família Multigênica , Análise de Sequência com Séries de Oligonucleotídeos , Fases de Leitura Aberta , Coelhos , Vibrio cholerae/patogenicidade , Virulência/genética
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...