RESUMO
OBJECTIVES: The role of autophagy in the pathophysiology of otitis media with effusion (OME) remains unclear, particularly regarding the difference between pediatric and adult patients. The present study analyzed the expression levels of autophagy-associated mRNAs in effusion fluids obtained from pediatric and adult patients with OME. MATERIALS AND METHODS: Middle ear fluid samples were collected from 76 pediatric patients and 41 adult patients with OME, and the levels of mRNAs encoding autophagy-related genes were measured using real-time reverse transcription-polymerase chain reaction. The relationships between the levels of autophagy-associated mRNAs and the frequency of ventilation tube insertion, the characteristics of middle ear fluid, and the results of bacterial culture were analyzed. RESULTS: Autophagy-associated mRNAs were present in the effusion fluid of all patients. The level of Beclin-1 mRNA was significantly lower in pediatric than in adult patients, regardless of the frequency of surgery or fluid characteristics (p < 0.05). CONCLUSION: Autophagy-associated mRNAs were expressed in effusion fluids of both pediatric and adult patients with OME. However, the level of Beclin-1 mRNA was significantly lower in the effusion fluid of pediatric than adult patients.
Assuntos
Proteína Beclina-1/genética , Otite Média com Derrame/genética , RNA Mensageiro/genética , Adulto , Idoso , Autofagia/genética , Criança , Pré-Escolar , Orelha Média/patologia , Orelha Média/cirurgia , Exsudatos e Transudatos/metabolismo , Exsudatos e Transudatos/microbiologia , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Ventilação da Orelha Média/efeitos adversos , Ventilação da Orelha Média/métodos , Otite Média/complicações , Otite Média/patologia , Otite Média/cirurgia , Otite Média com Derrame/diagnóstico , Otite Média com Derrame/microbiologia , Otite Média com Derrame/cirurgia , Reação em Cadeia da Polimerase em Tempo Real/métodosRESUMO
INTRODUCTION: We present a rare case of chylothorax associated with an intrathoracic goiter in Graves' disease that was treated with radioactive iodine. CASE REPORT: A 23-year-old woman with Graves' disease was referred to our clinic with a pleural effusion, dyspnea, characteristic bilateral proptosis, and a diffuse goiter. The pleural fluid biochemistry was consistent with chylothorax. However, the chylothorax did not decrease with conservative therapy. Therefore, RAI was administered. Subsequently, the chylothorax and goiter improved more quickly than expected. CONCLUSIONS: This case illustrates that chylothorax associated with a substernal goiter in Graves' disease can be treated successfully with radioactive iodine instead of surgery.
RESUMO
In the present study, we performed immunohistochemistry using antibodies directed against TRPCs to study the localizations of these channels in rat hippocampus. The pyramidal cell bodies of CA1-3 areas and the granule cell bodies of the dentate gyrus were immunoreactive for TRPC1, TRPC3, TRPC4 and TRPC5. On the other hand, TRPC6 exhibited the cloud-like neuropil staining only in the molecular layer of the dentate gyrus. As a whole, the present study has clearly shown the localization of TRPCs in rat hippocampus and may provide useful data for the future investigations on the structural and functional properties of TRPCs.
