Clinical effects of different etching modes for universal adhesives: a systematic review and meta-analysis.

BACKGROUND: To evaluate the clinical performance of universal adhesives in etch-and-rinse or self-etch application modes through meta-analysis. METHODS: A literature search was performed by two reviewers in the PubMed, Cochrane Library, and Embase databases (from January 2000 to March 2020). A total of 2,516 non-replicated records were identified and filtered. Studies that evaluated the clinical performance of universal adhesives using etch-and-rinse or self-etch mode were included. RevMan 5.3.5 (Cochrane, London, UK) was used to perform the meta-analysis. RESULTS: A total of 13 studies were included in the meta-analysis. The retention rates were higher in etch-and-rinse groups compared with self-etch groups [odds ratio (OR) =0.35, 95% confidence interval (CI): 0.18-0.71, P=0.003]. The etch-and-rinse approach also had better performance in marginal adaptation (OR =0.49, 95% CI: 0.36-0.67, P<0.001) and marginal staining (OR =0.49, 95% CI: 0.36-0.66, P<0.001). The current data showed a very low incidence rate of secondary caries or postoperative sensitivity, and there were no significant differences in the incidence rates between the etch-and-rinse groups and self-etch groups. DISCUSSION: The current evidence shows that, compared with self-etch approach, the etch-and-rinse approach for universal adhesives provides improved clinical outcomes in terms of retention rates, marginal adaptation, and marginal staining.

Dental caries and associated factors among Chinese children and adolescents: A protocol for systematic review and meta-analysis.

BACKGROUND: Dental caries is a common disease under the action of many factors. Dental caries may occur in all age groups, among which children and adolescents are at high risk of dental caries. Early identification of the risk factors of dental caries is very important for clinical staff to prevent and intervene as soon as possible and reduce the incidence of dental caries. Although Chinese scholars have studied and summarized the risk factors of dental caries in children and adolescents, the conclusions are different. Therefore, in this study, meta-analysis was used to summarize the risk factors of dental caries in Chinese children and adolescents, and to explore the characteristics of high-risk groups of dental caries, so as to provide reference for early detection and prevention of dental caries. METHODS: Medical specialty databases like PubMed, Embase, Cochrane Library, Web of Science, China Biology Medicine Database, China National Knowledge Infrastructure, China Science and Technology Journal Database, and Wanfang Database were consistently and exhaustively searched. According to the method of evidence-based medicine, the inclusion and exclusion criteria were established, and the meta-analysis of all eligible research results was carried out by using Review Manager 5.3 software. RESULTS: We will disseminate the findings of this systematic review and meta-analysis via publications in peer-reviewed journals. CONCLUSIONS: Our study was carried out to estimate the pooled prevalence of dental caries and its associated factors among Chinese children and adolescents. OSF REGISTRATION NUMBER: DOI 10.17605/OSF.IO/RA9D6.

Antibacterial effect and bond strength of a modified dental adhesive containing the peptide nisin.

This study attempted to incorporate the antibacterial peptide nisin into an etch-and-rinse dental adhesive to evaluate the antibacterial activity of the modified adhesive against Streptococcus mutans and the bond strength. Single Bond 2 was used as a negative control, and nisin was incorporated at 1%, 3%, and 5% (w/v). The antibacterial activity against S. mutans was evaluated using the film contact test, the agar diffusion test, XTT assays and confocal laser scanning microscopy (CLSM). The microtensile bond strength (µTBS) of the modified dental adhesive was also evaluated. The cured nisin-incorporated dental adhesive exhibited a significant inhibitory effect on the growth of S. mutans (P<0.05), and the inhibitory effect was strengthened as the nisin concentration increased (P<0.05). However, no significant differences in the agar diffusion test were found for the cured nisin-incorporated adhesives compared with the control group. Based on XTT results and CLSM images, the cured nisin-incorporated adhesive interfered with the adherence of S. mutans and the integrity of its biofilms (P<0.05). Compared with the control group, the 1% nisin group did not exhibit a significant difference in µTBS (P>0.05), whereas the 3% and 5% nisin groups displayed decreased bond strength (P<0.05).