How do environmentally friendly antifouling alkaloids affect marine fouling microbial communities?

Most previous studies on antifouling (AF) agents have focused on the influence of biofilm formation constituted by single or multiple cultured microbial species, and very few studies have analyzed the relationship between environmentally friendly AF compounds and marine fouling microbial communities (MFMCs). This is the first investigation of the impact of three environmentally friendly alkaloids (5-chlorosclerotiamide, circumdatin F and notoamide C) produced by the deep-sea-derived fungus Aspergillus westerdijkiae on MFMCs using high-throughput Illumina sequencing in a field test. The results of this study showed that the three alkaloids could significantly decrease the coverage of marine microflora (p < 0.05) and affect the composition and diversity of MFMCs on polyvinyl chloride (PVC) plates. Furthermore, 5-chlorosclerotiamide and notoamide C could completely inhibit many macrofouler-inductive-bacteria, such as Pseudoalteromonas and Pseudomonas, and promote the anti-macrofouler-bacteria, such as Winogradskyella, from 0.21% to more than 10% of the MFMCs on PVC plates. These results suggested that 5-chlorosclerotiamide and notoamide C could influence the compositions of MFMCs and make it unfavorable for the settlement of macrofoulers, by reducing the abundance of macrofouler-inductive-bacteria and promoting the percentage of anti-macrofouler-bacteria on PVC plates. The present study provides a new way to evaluate the effect of environmentally friendly AF compounds and obtain a better understanding of the antifouling process.

Continual production of glycerol from carbon dioxide by Dunaliella tertiolecta.

Microalgae have high photosynthetic efficiencies and produce many valuable compounds from carbon dioxide. The Dunaliella genus accumulates glycerol, yet no commercial process currently exists for glycerol production from this microalga. Here it was found that in addition to intracellular accumulation, Dunaliella tertiolecta also releases glycerol into the external medium continuously, forming a large and stable carbon pool. The process is not affected by nutrient starvation or onset of cell death. Carbon dioxide was fixed at a constant rate, the bulk of it being channelled to extracellular glycerol (82%), resulting in enhanced photosynthetic carbon assimilation of 5 times that used for biomass production. The final extracellular glycerol concentration was 34 times the maximum concentration of intracellular glycerol; the latter declined further during cell death. Findings from this work will assist in the development of a bioconversion process to produce glycerol using D. tertiolecta without the need for cell harvest or disruption.