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1.
J Helminthol ; 77(1): 39-42, 2003 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-12590663

RESUMO

A preliminary study to detect human worm carriers of Taenia solium in Papua (Irian Jaya), Indonesia was carried out using stool examinations for the detection of copro-antigens and adult proglottids after chemotherapy, and confirmation by mitochondrial DNA analysis using expelled proglottids and metacestodes developed in NOD/Shi-scid mice from eggs of expelled proglottids. Approximately 8.6% of the local population in Kama (5/58), 1 km from the local capital city centre, Wamena, were confirmed to harbour adult T. solium using these techniques.


Assuntos
Cysticercus , Enteropatias Parasitárias/epidemiologia , Taenia solium , Teníase/epidemiologia , Adolescente , Adulto , Idoso , Animais , Antígenos de Helmintos/sangue , Portador Sadio , Cisticercose/diagnóstico , Cisticercose/epidemiologia , Cisticercose/imunologia , Cysticercus/imunologia , DNA de Helmintos/análise , Feminino , Humanos , Enteropatias Parasitárias/diagnóstico , Enteropatias Parasitárias/imunologia , Masculino , Camundongos , Camundongos Endogâmicos NOD , Pessoa de Meia-Idade , Papua Nova Guiné/epidemiologia , Parasitologia/métodos , Prevalência , Taenia solium/genética , Taenia solium/imunologia , Teníase/diagnóstico
2.
J Helminthol ; 76(4): 311-4, 2002 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-12498635

RESUMO

Serology (ELISA and immunoblot) using native glycoproteins, affinity purified glycoproteins, and a recombinant antigen is known to be highly specific to Taenia solium cysticercosis in humans and pigs. These techniques were applied for dogs in the highly endemic area of cysticercosis in Papua (Irian Jaya), Indonesia. Analysis of dog sera by both ELISA and immunoblot revealed 7 of 64 dogs were highly positive. Examination of two sero-positive dogs revealed cysticerci of T. solium in the brain and heart of these dogs. Mitochondrial DNA analysis confirmed that they were the same as T. solium previously confirmed from pigs and biopsies from local people from Irian Jaya. It is suggested that the life cycle of T. solium may be completed not only between humans and pigs but also between humans and dogs.


Assuntos
Cisticercose/veterinária , Vetores de Doenças , Doenças do Cão/diagnóstico , Taenia/isolamento & purificação , Animais , Antígenos de Helmintos/imunologia , Cisticercose/diagnóstico , DNA de Helmintos/análise , DNA Mitocondrial/análise , Cães , Ensaio de Imunoadsorção Enzimática , Humanos , Immunoblotting , Proteínas Recombinantes/imunologia , Suínos/parasitologia , Taenia/classificação , Taenia/genética
3.
Artigo em Inglês | MEDLINE | ID: mdl-12971481

RESUMO

Detection of seven specific bands by immunoblot (IB) using glycoproteins (GPs) purified by lentil-lectin affinity chromatography has been the gold-standard for neurocysticercosis (NCC) serodiagnosis since 1989. However, due to the presence of contaminants, it was impossible to apply the GPs to ELISA. Our group at Asahikawa Medical College (AMC) succeeded in purifying the GPs by preparative isoelectric focusing; these higher quality GPs were suitable for ELISA. Based on the results of both IB and ELISA testing, developed at AMC for a field survey in Irian Jaya, it became evident that that area had pandemic NCC. We found many NCC patients, pigs full of cysts, and one dog infected with two cysts: these findings were based on serology. Recently, we conducted another survey to detect of the worm carriers of T. solium. Three of the 38 local people were positive by copro-antigen specific to Taenia species; these three patients expelled segments of Taenia spp and these were confirmed as those of T. solium by mitochondrial DNA analysis. When viable eggs of any taeniid species could be obtained, they can be developed into metacestodes in NOD-scid mice; it then becomes possible to analyze morphological dynamics, metacestode antigenicity, the efficacy of new metacestocidal drugs, and mitochondrial DNA. Mitochondrial DNA analysis of the specimens obtained in Irian Jaya was compared with that of other isolates worldwide. T. solium is now divided into two genotypes: the Asian type, and the Africa-American type. Some aspects of the pathological differences between the Asian and Africa-American types and the antigenic components of these two types are discussed.


Assuntos
Teníase/prevenção & controle , Animais , Antígenos de Helmintos/genética , Ásia/epidemiologia , Modelos Animais de Doenças , Ensaio de Imunoadsorção Enzimática , Humanos , Camundongos , Camundongos SCID , Polimorfismo Genético , Testes Sorológicos , Suínos , Taenia solium/imunologia , Teníase/diagnóstico , Teníase/epidemiologia , Teníase/veterinária
5.
Artigo em Inglês | MEDLINE | ID: mdl-12041608

RESUMO

Cysticercosis caused by the larval stage, cysticercus or cysticerci, of the pork tapeworm Taenia solium was recognized at first in Bali and in Paniai District, Irian Jaya (Papua), Indonesia in the 1970s. In the 1990s a rapid increase in the number of the cases of epileptic seizures and burns in Jayawijaya district, eastern Papua, was observed. There were a total of 1,120 new cases of burns (7.0%) and 293 new cases of epileptic seizures (1.8%) from 15,939 local people during 1991-1995. Both histopathological examination and mitochondrial DNA analysis of resected cysts from patients and pigs revealed cysticerci of T. solium. Antibody responses highly specific to cysticercosis were revealed in approximately 67% and 65% of persons respectively with epileptic seizures and with subcutaneous nodules. Therefore, most cases of epileptic seizures and burns were considered to be associated with cysticercosis in Papua. Additional serologically data from Bali showed that 13.5% of epileptic seizures (10/74) and 12.6% of asymptomatic individuals (94/746) were supposed having been exposed to T. solium. Histopathological evaluation of 80,000 tissue samples in East Java revealed that nine were cysticercosis. All cases were non-moslems and from two ethnic groups, Chinese and Balinese. Epidemiological data on cysticercosis are not available from other provinces of Indonesia, although cases of cysticercosis are occasionally reported. Therefore, other intensive epidemiological studies are strongly recommended, especially covering the eastern part of Indonesia.


Assuntos
Queimaduras/parasitologia , Cisticercose/epidemiologia , Epilepsia/parasitologia , Doenças dos Suínos/epidemiologia , Taenia/crescimento & desenvolvimento , Adulto , Idoso , Animais , Queimaduras/etiologia , Cisticercose/complicações , Cysticercus/crescimento & desenvolvimento , Epilepsia/etiologia , Feminino , Humanos , Immunoblotting , Indonésia/epidemiologia , Masculino , Pessoa de Meia-Idade , Suínos , Doenças dos Suínos/parasitologia , Zoonoses/parasitologia
6.
Trans R Soc Trop Med Hyg ; 94(1): 46-50, 2000.
Artigo em Inglês | MEDLINE | ID: mdl-10748897

RESUMO

Historically, neurocysticercosis (NCC) caused by the larval stage, cysticercus or cysticerci, of the pork tapeworm Taenia solium was recognized in Paniai District, western Irian Jaya Province, Indonesia, in the early 1970s. In the 1990s, we observed a rapid increase in the number of cases of epileptic seizures and burns in Assologaima Sub-District, Jayawijaya District, eastern Irian Jaya. There were totals of 1120 new cases of burns and 293 new cases of epileptic seizures during 1991-95 in Assologaima where the number of inhabitants was 15,939. Histopathological examination of resected cysts from patients and a pig revealed that they were cysticerci of T. solium. DNA analysis of these cysts revealed that the nucleotide sequences of 391 base-pair fragments of the mitochondrial cytochrome c oxidase subunit 1 gene were exactly the same in those from patients and the pig. Although 3 of 391 base-pair fragments might differ from that of T. solium reported previously, there were no differences in the amino-acid sequences. Approximately 67% and 65% of persons with epileptic seizures and with subcutaneous nodules, respectively, showed antibody responses highly specific to cysticercosis. Therefore, most cases of epileptic seizures and burns were considered to be associated with cysticercosis in Irian Jaya.


Assuntos
Queimaduras/epidemiologia , Cysticercus , Reservatórios de Doenças , Epilepsia/epidemiologia , Neurocisticercose/epidemiologia , Adolescente , Adulto , Distribuição por Idade , Idoso , Idoso de 80 Anos ou mais , Animais , Criança , Pré-Escolar , Epilepsia/patologia , Feminino , Genes de Helmintos , Humanos , Immunoblotting , Incidência , Indonésia/epidemiologia , Lactente , Masculino , Pessoa de Meia-Idade , Neurocisticercose/diagnóstico , Neurocisticercose/transmissão , Testes Sorológicos , Suínos , Taenia/genética
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