Actinobacterial peroxidase-mediated biodeterioration of hazardous explosive, 2, 4, 6, trinitrophenol by in silico and in vitro approaches.

Explosives are perilous and noxious to aquatic biota disrupting their endocrinal systems. Supplementarily, they exhibit carcinogenic, teratogenic and mutagenic effects on humans and animals. Henceforth, the current study has been targeted to biotransform the explosive, 2, 4, 6 trinitrophenol (TNP) by wetland peroxidase from Streptomyces coelicolor. A total peroxidase yield of 20,779 mg/l with 51.6 folds of purification was observed. In silico molecular docking cum in vitro appraisals were accomplished to assess binding energy and interacting binding site residues of peroxidase and TNP complex. TNP required a minimal binding energy of-6.91 kJ/mol and was subjected to biodeterioration (89.73%) by peroxidase in purified form, with 45 kDa and a similarity score of 34 by MASCOT protein analysis. Moreover, the peroxidase activity was confirmed with Zymogram analysis. Characterization of peroxidase revealed that optimum values of pH and temperature as 6 and 40 °C, respectively, with their corresponding stability varying from 3.5 to 7. Interestingly, the kinetic parameters such as Km and Vmax on 2,2'-azino-bis 3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and H2O2 were 19.27 µm and 0.41 µm/min; 21.4 µm and 0.1 µm/min, respectively. Among the diverse substrates, chemicals and trace elements, ABTS (40 mM), citric acid (5 mM) and Fe2+ (5 mM) displayed the highest peroxidase activity. Computational docking and in vitro results were corroborative and UV-Vis spectroscopy, HPLC, FTIR and GC-MS indicated the presence of simple metabolites of TNP such as nitrophenols and benzoquinone, showcasing the efficacy of S. coelicolor peroxidase to biotransform TNP. Henceforth, the current study offers a promising channel for biological treatment of explosive munitions, establishing a sustainable green earth.

Comprehensive review on recent production trends and applications of biochar for greener environment.

The suitability of biochar as a supplement for environmental restoration varies significantly based on the type of feedstocks used and the parameters of the pyrolysis process. This study comprehensively examines several aspects of biochar's potential benefits, its capacity to enhance crop yields, improve nutrient availability, support the co-composting, water restoration and enhance overall usage efficiency. The supporting mechanistic evidence for these claims is also evaluated. Additionally, the analysis identifies various gaps in research and proposes potential directions for further exploration to enhance the understanding of biochar application. As a mutually advantageous approach, the integration of biochar into agricultural contexts not only contributes to environmental restoration but also advances ecological sustainability. The in-depth review underscores the diverse suitability of biochar as a supplement for environmental restoration, contingent upon the specific feedstock sources and pyrolysis conditions used. However, concerns have been raised regarding potential impacts on human health within agricultural sectors.

Critical review of biochemical pathways to transformation of waste and biomass into bioenergy.

In recent years, biofuel or biogas have become the primary source of bio-energy, providing an alternative to conventionally used energy that can meet the growing energy demand for people all over the world while reducing greenhouse gas emissions. Enzyme hydrolysis in bioethanol production is a critical step in obtaining sugars fermented during the final fermentation process. More efficient enzymes are being researched to provide a more cost-effective technique during enzymatic hydrolysis. The exploitation of microbial catabolic biochemical reactions to produce electric energy can be used for complex renewable biomasses and organic wastes in microbial fuel cells. In hydrolysis methods, a variety of diverse enzyme strategies are used to promote efficient bioethanol production from various lignocellulosic biomasses like agricultural wastes, wood feedstocks, and sea algae. This paper investigates the most recent enzyme hydrolysis pathways, microbial fermentation, microbial fuel cells, and anaerobic digestion in the manufacture of bioethanol/bioenergy from lignocellulose biomass.

Recent approaches and advanced wastewater treatment technologies for mitigating emerging microplastics contamination - A critical review.

Microplastics have been identified as an emerging pollutant due to their irrefutable prevalence in air, soil, and particularly, the aquatic ecosystem. Wastewater treatment plants (WWTPs) are seen as the last line of defense which creates a barrier between microplastics and the environment. These microplastics are discharged in large quantities into aquatic bodies due to their insufficient containment during water treatment. As a result, WWTPs are regarded as point sources of microplastics release into the environment. Assessing the prevalence and behavior of microplastics in WWTPs is therefore critical for their control. The removal efficiency of microplastics was 65 %, 0.2-14 %, and 0.2-2 % after the successful primary, secondary and tertiary treatment phases in WWTPs. In this review, other than conventional treatment methods, advanced treatment methods have also been discussed. For the removal of microplastics in the size range 20-190 µm, advanced treatment methods like membrane bioreactors, rapid sand filtration, electrocoagulation and photocatalytic degradation was found to be effective and these methods helps in increasing the removal efficiency to >99 %. Bioremediation based approaches has found that sea grasses, lugworm and blue mussels has the ability to mitigate microplastics by acting as a natural trap to the microplastics pollutants and could act as candidate species for possible incorporation in WWTPs. Also, there is a need for controlling the use and unchecked release of microplastics into the environment through laws and regulations.

Ecological disturbances and abundance of anthropogenic pollutants in the aquatic ecosystem: Critical review of impact assessment on the aquatic animals.

Anthropogenic toxins are discharged into the environment and distributed through a variety of environmental matrices. Trace contaminant detection and analysis has advanced dramatically in recent decades, necessitating further specialized technique development. These pollutants can be mobile and persistent in small amounts in the environment, and ecological receptors will interact with it. Despite the fact that few researches have been undertaken on invertebrate exposure, accumulation, and biological implications, it is apparent that a wide range of pollutants can accumulate in the tissues of aquatic insects, earthworms, amphipod crustaceans, and mollusks. Due to long-term stability during long-distance transit, a number of chemical and microbiological agents that were not previously deemed pollutants have been found in various environmental compartments. The uptake of such pollutants by the aquatic organism is done through the process of bioaccumulation when dangerous compounds accumulate in living beings while biomagnification is the process of a pollutant becoming more hazardous as it moves up the trophic chain. Organic and metal pollution harms animals of every species studied so far, from bacteria to phyla in between. The environmental protection agency says these poisons harm humans as well as a variety of aquatic organisms when the water quality is sacrificed in typical wastewater treatment systems. Contrary to popular belief, treated effluents discharged into aquatic bodies contain considerable levels of Anthropogenic contaminants. This evolution necessitates a more robust and recent advancement in the field of remediation and their techniques to completely discharge the various organic and inorganic contaminants.

Sustainability and carbon neutralization trends in microalgae bioenergy production from wastewater treatment: A review.

Reducing CO2 emissions using biomass is gaining popularity as an environmentally friendly strategy. Due to high growth rates, low production costs, and ability to withstand harsh conditions, microalgae have become quite popular. Microalgae may also undertake photosynthesis, converting CO2 and solar energy into sugar before becoming biomass, making them an excellent source of renewable and promising biofuels. CO2 sequestration and biofixation was utilized to compare the synthesis of biodiesel as a third-generation biofuel from various types of wastewater was also used as a source for the algal cultivation. This review article focuses on recent developments, research discoveries in the field of microalgal CO2 capture modification and the optimization of conversion efficiency. This review is intended to serve as a helpful and reference for the use of wastewater treatment with microalgae to collect CO2. The overarching objective of this study is to assist wastewater treatment systems in achieving carbon neutrality.

Graphene materials: Armor against nosocomial infections and biofilm formation - A review.

Graphene has revolutionized the field of energy and storage sectors. Out of the total number of nosocomial infections diagnosed all around the world, the majority of the cases (around 70%) are found to be due to the medical device or assistance utilized while treating the patient. Combating these diseases is vital as they cause a nuisance to the patients and medical practitioners. Coatings of graphene and its derivatives hold the key to the formation of special surfaces that can rupture microbial cells using their sharp edges, ultimately leading to nuclear and cellular fragmentation. Their incorporation as a whole or as a part in the hospital apparel and the medical device has aided medical practitioners to combat many nosocomial diseases. Graphene is found to be highly virulent with broad-spectrum antimicrobial activity against nosocomial strains and biofilm formation. Their alternate mode of action like trapping and charge transfer has also been discussed well in the present review. The various combinational forms of graphene with its conjugates as a suitable agent to combat nosocomial infections and a potential coating for newer challenges like COVID-19 infections has also been assessed in the current study. Efficiency of graphene sheets has been found to be around 89% with a reaction time as less as 3 h. Polymers with graphene seem to have a higher potency against biofilm formation. When combined with graphene oxide, silver nanoparticles provide 99% activity against nosocomial pathogens. In conclusion, this review would be a guiding light for scientists working with graphene-based coatings to unfold the potentials of this marvelous commodity to tackle the present and future pandemics to come.

Emerging nanotechnology based advanced techniques for wastewater treatment.

The increasing trend of industrialization leads to tremendous release of industrial effluents. Waste water treatment is one of the important sectors to focus in order to overcome the most threatening issue of waste disposal and to ensure sustainability. Sustainable and energy efficient treatment methods are the attractive technologies for their current implementation of waste management. Even though the existing technologies are effective, unsustainability makes them unfit for their extended applications. Conventional and advanced technologies have been extensively implemented for the treatment of wide spectrum of effluents. Hybrid technologies including chemical and biological methods also emerging as promising technologies but secondary sludge generation is still unaddressed. Even though effectiveness of biochar varies over type of contaminants, cost-effectiveness and eco-friendly nature extended their applications in waste management. Nanotechnology and membrane technology are the promising and emerging areas of interest due to their widespread applications in waste water treatment. Carbon nano structures, nano filters, graphene, nano magnets modified with activated carbon are the potential candidates for the treatment. The present review demonstrates the emerging treatment technologies with special focus to nano based waste water treatment methods.

Assessment, characterization, and quantification of microplastics from river sediments.

Microplastic (MP), as a pollutant, is currently posing a biological hazard to the aquatic environment. The study aims to isolate, quantify, and characterize the MP pollutants in sediment samples from 14 study sites at Kaveri River, Killa Chinthamani, Tiruchirappalli, South India. With Sediment-MP Isolation (SMI) unit, density separation was done with a hydrogen peroxide solution. Four forms of MPs namely, fragments, films, foams, and fibers with orange, white, green, and saffron red were observed. The plenitude and distribution of four forms of MPs and natural substrates were geometrically independent, with large amounts of microfragments within the research region accounting for 79.72% variation by Principal Component Analysis. FT-IR analyses of MPs showed the presence of polyamide, polyethylene, polyethylene glycol, polyethylene terephthalate, polypropylene, and polystyrene. Additionally, the scanning electron microscopic analysis revealed that the MPs have differential surface morphology with rough surfaces, porous structures, fissures, and severe damage. Most MPs comprised Si, Mg, Cu, and Al, according to energy dispersive X-ray analyses. The combined SMI, instrumental analyses and evaluation (heat map) of MPs in river sediments help assess contamination levels and types of MPs. The findings might provide an insight into the status of MPs in Kavery River sediments that could help in formulating regulations for MPs reduction and contamination in rivers eventually to protect the environment.

Detoxification of coir pith through refined vermicomposting engaging Eudrilus eugeniae.

Hazardous coir industrial waste, coir pith has been subjected to 50 days vermicomposting with Eudrilus eugeniae by amending nitrogenous legume plant, Gliricidia sepium together with cattle dung in different combinations, after 21 days precomposting using Pleurotus sajor-caju spawn. An increase in electrical conductivity, total NPK and calcium, and a decrease in organic matter, total organic carbon, C/N ratio, C/P ratio and total phenolic content in the final vermicompost were observed. Dehydrogenase, urease and cellulase activity peaked up to 30 days of vermicomposting and then declined. The phytotoxicity studies with Brassica juncea, C/N ratio and enzyme activities confirmed the stability and maturity of vermicompost. The results also demonstrated that the 2:3:1 ratio (coir pith + Gliricidia sepium + cow dung) is a suitable effective combination for nutrient-rich (N: 2.43%; P: 0.92%; K: 2.09%) vermicompost production. The total phenolic contents declined during the vermicomposting with a lower final content of 21.26 mg/g GAE in 2:3:1 combination of substrates from the initial level (105.56 mg/g GAE). Besides, the concentration of total phenol contents inversely related to the germination index of Brassica juncea (r = -0.761), indicating that the phenolic content could also play an important role in phytotoxicity. Growth and fecundity of Eudrilus eugeniae in 2:3:1 combination revealed the acceptability and rapid decomposition of coir pith substrate into vermifertilizer.

A critical review of advanced nanotechnology and hybrid membrane based water recycling, reuse, and wastewater treatment processes.

One of the modern challenges is to provide clean and affordable drinking water. Water scarcity is caused by the growing population in the world and pollutants contaminate all remaining water sources. Innovative water treatment solutions have been provided by nanotechnology. Microorganisms, organic suspensions, and inorganic heavy metal ions, among other things, are common water contaminants. Since antiquity, a wide range of water clean-up methods have been employed to address this issue. Breakthroughs in water purification procedures have occurred during the previous four decades, with the most significant one being the use of nanomaterials and nanomembranes. Nanoparticles and nanomembranes (polymeric membranes) have recently been used in engineered materials (TiO2, ZnO, CuO, Ag, CNT's and mixed oxide nanoparticles, for example). Engineered nanomembranes, nanocomposites and nanoparticles have been used in this review article's discussion of water purification technologies. The review also discusses the risk and solutions of using nanoparticles and nanocomposites in the future.

Efficiency of microbial fuel cells in the treatment and energy recovery from food wastes: Trends and applications - A review.

The rising global population and their food habits result in food wastage and cause an obstacle in its treatment and disposal. Due to the rapid shift in the lifestyle of the human population and urbanization, almost one-third of the food produced is wasted from various sectors like domestic sources, agricultural sectors, and industrial sectors. These food resources squandered are rich in organic biomolecules which can cause complications upon direct disposal in the environment. Conventional disposal methods like composting, landfills and incineration demand high costs besides causing severe environmental and health issues. To overcome these demerits of the conventional methods and to avoid the loss of rich organic food resources, there is an immediate need for a sustainable and eco-friendly solution for the valorization of the food wastes. Microbial fuel cells (MFCs) are gaining attention, due to their ideal approach in the production of electricity and parallel treatment of organic food wastes. The MFCs are significant as an innovative approach using microorganisms and oxidizing the organic food wastes into bio-electricity. In this review, the recent advancements and practices of the MFCs in the field of food waste treatment and management along with electricity production are discussed. The major outcome of this work highlights the setting up of MFC for the treatment of higher volumes of food waste residues and enhancing the bioelectricity production in an optimal condition. For further improvements in the food waste treatments using MFCs, greater understanding and more research needs are to be focused on the commercialization, different operational modes, operational types, and low-cost fabrication coupled with careful examination of scale-up factors.

Insights of CRISPR-Cas systems in stem cells: progress in regenerative medicine.

Regenerative medicine, a therapeutic approach using stem cells, aims to rejuvenate and restore the normalized function of the cells, tissues, and organs that are injured, malfunctioning, and afflicted. This influential technology reaches its zenith when it is integrated with the CRISPR-Cas (clustered regularly interspaced short palindromic repeats-CRISPR associated) technology of genome editing. This tool acts as a programmable restriction enzyme system, which targets DNA as well as RNA and gets redeployed for the customization of DNA/RNA sequences. The dynamic behaviour of nuclear manipulation and transcriptional regulation by CRISPR-Cas technology renders it with numerous employment in the field of biologics and research. Here, the possible impact of the commonly practiced CRISPR-Cas systems in regenerative medicines is being reviewed. Primarily, the discussion of the working mechanism of this system and the fate of stem cells will be scrutinized. A detailed description of the CRISPR based regenerative therapeutic approaches for a horde of diseases like genetic disorders, neural diseases, and blood-related diseases is elucidated.

Phycoremediation of wastewater for pollutant removal: A green approach to environmental protection and long-term remediation.

Surface and water bodies in many parts of the world are affected due to eutrophication, contamination and depletion. The approach of wastewater treatment using algae for eliminating nutrients and other pollutants from domestic wastewater is growing interest among the researchers. However, sustainable treatment of the wastewater is considered to be important in establishing more effective nutrient and pollutant reduction using algal systems. In comparison to the conventional method of remediation, there are opportunities to commercially viable businesses interest with phycoremediation, thus by achieving cost reductions and renewable bioenergy options. Phycoremediation is an intriguing stage for treating wastewater since it provides tertiary bio-treatment while producing potentially valuable biomass that may be used for a variety of applications. Furthermore, the phycoremediation provides the ability to remove heavy metals as well as harmful organic substances, without producing secondary contamination. In this review, the role of microalgae in treating different wastewaters and the process parameters affecting the treatment and future scope of research have been discussed. Though several algae are employed for wastewater treatment, species of the genera Chlamydomonas, Chlorella, and Scenedesmus are extensively utilized. Interestingly, there is a vast scope for employing algal species with high flocculation capacity and adsorption mechanisms for the elimination of microplastics. In addition, the algal biomass generated during phycoremediation has been found to possess high protein and lipid contents, promising their exploitation in biofuel, food and animal feed industries.

Chemico-nanotreatment methods for the removal of persistent organic pollutants and xenobiotics in water - A review.

While the technologies available today can generate high-quality water from wastewater, the majority of the wastewater treatment plants are not intended to eliminate emerging xenobiotic pollutants, pharmaceutical and personal care items. Most endocrine disrupting compounds (EDCs) and personal care products (PPCPs) are more arctic than most regulated pollutants, and several of them have acid or critical functional groups. Together with the trace occurrence, EDCs and PPCPs create specific challenges for removal and subsequent improvements of wastewater treatment plants. Various technologies have been investigated extensively because they are highly persistent which leads to bioaccumulation. Researchers are increasingly addressing the human health hazards of xenobiotics and their removal. The emphasis of this review was on the promising methods available, especially nanotechnology, for the treatment of xenobiotic compounds that are accidentally released into the setting. In terms of xenobiotic elimination, nanotechnology provides better treatment than chemical treatments and their degradation mechanisms are addressed.

Emerging nano-structured innovative materials as adsorbents in wastewater treatment.

Water supply around the globe is struggling to meet the rapidly increasing demand by the population, drastic changes in climate and degrading water quality. Even though, many large-scale methods are employed for wastewater treatment they display several negative impacts owing to the presence of pollutants. Technological innovation is required for integrated water management with different groups of nanomaterials for the removal of toxic metal ions, microbial disease, organic and inorganic solutes. The method of manipulating atoms on a nanoscale is nanotechnology. Nanomembranes are used in nanotechnology to soften water and eliminate physical, chemical and biological pollutants. The present review concentrates on various nanotechnological approaches in wastewater remedy, mechanisms involved to promote implementation, benefits and limitations in comparison with current processes, properties, barriers and commercialization research needs. Also the review identifies opportunities for further exploiting the exclusive features for green water management by following the advances in nanotechnology.

Earthworm intervened nutrient recovery and greener production of vermicompost from Ipomoea staphylina - An invasive weed with emerging environmental challenges.

The invasive weed, Ipomoea staphylina (IS) with cow dung (CD) and mushroom spent straw (MS) in four different combinations (IS:CD:MS), V1 (1:1:0), V2 (2:1:1), V3 (1:0:1) and V4 (1:1:1) were pre-decomposed for 21 days followed by 50 days vermicomposting using Eudrilus eugeniae in triplicates in order to alleviate and to utilize the weed biomass in an environment-friendly manner. The contents of organic matter, organic carbon, cellulose, lignin, C/N and C/P ratios showed a decrease, while electrical conductivity, total NPK, calcium, sodium, and nitrate-nitrogen showed a significant increase in vermicompost over control. Water-soluble organic carbon to organic nitrogen ratio and C/N ratio in V1 (0.52 and 17.55) and V4 (0.43 and 16.56), respectively, were in conformity with the maturity of vermicomposts. Scanning electron micrographs of the end products clearly showed more fragmented, fine, and porous particles in vermicompost. Copper, chromium, cadmium, lead, and zinc in vermicomposts were below the permissible limits. Dehydrogenase, acid phosphatase, alkaline phosphatase, cellulase, and protease activities were significantly higher in V4 than other treatments, implying the role of MS and CD addition during vermicomposting. Though V3 combination supported worm biomass, V4 combination was found to favor the fecundity of Eudrilus eugeniae. Results reveal that 1:1:1 combination of SI + CD + MS (V4) is suitable for utilizing the weed biomass for vermicompost production and nutrient recovery. From the biomass of environmentally problematic weed, Ipomoea staphylina, nutrient-rich vermicompost can be produced through vermitechnology for sustainable environmental management and agriculture.

Aqueous two-phase partitioning and characterization of xylanase produced by Streptomyces geysiriensis from low cost lignocellulosic substrates.

Microbial production of xylanase is gaining the commercial importance, due to its wide range of applications from paper and pulp to food and feed industries. Streptomyces geysiriensis was used for the production of extracellular xylanase from lignocellulosic substrates such as rice bran and saw dust, under solid-state fermentation. The influence of pH, temperature and incubation period for the maximum production of xylanase was investigated with 1:2 (w/v) of substrate to moisture ratio at 100 rpm shaking conditions. The maximum production was recorded after 5 days of fermentation with pH 8.0 at 40 °C. The scale-up was done based on the results of optimized parameters using 3 L Applikon autoclavable bioreactor with maximum yield of 186 U/ml after 4 days of fermentation. Extracellular xylanase was separated by partitioning in aqueous two-phase system consisting of 20% polyethylene glycol 6000 and 12% K2HPO4 with maximum yield of 93.97%. The investigation of the effect of pH and temperature and its incubation time showed that xylanase was retained its activity in a pH range of 6.5-8.5, with thermal stability from 20 °C to 60 °C up to 180 min. The presence of metal ions was found to inhibit the activity of xylanase especially Cu2+ and Zn2+. Xylanase was stable both at 4 °C and room temperature (35 °C) for 30 and 9 days respectively. The kinetic parameters Km (0.48 mg/ml) and Vmax (8.33 U/mg) were determined using birchwood xylan as substrate.

Enriched pressmud vermicompost production with green manure plants using Eudrilus eugeniae.

Vermicomposting of pressmud with cow dung and nitrogenous green manures (Gliricidia sepium and Leucaena leucocephala) was carried out using Eudrilus eugeniae (50 days). The reduction in pH, total organic carbon, C/N ratio, water-soluble organic carbon (Cws)/Norg and C/P ratios, and a pronounced increase in NPK contents and microbial population in vermicompost were observed. An enhanced TKN of 3.80% and 3.45% was recorded in vermicomposts of pressmud + cow dung + L. leucocephala (2:1:1) and pressmud + cow dung + G. sepium (2:1:1) respectively. The C/N and Cws/Norg ratios in vermicompost ranged from 11.86 to 16.66 and 0.53 to 1.33, respectively. The activity of dehydrogenase, urease, acid and alkaline phosphatase declined towards the end, indicating the progression of vermicompost maturity. The pressmud and green manure substrates promoted more biomass of E. eugeniae, while cow dung with green manure combination favored reproduction. The amendment of cow dung and green manure plants to pressmud (2:1:1 ratio) results in nutrient-enriched vermicompost production.

Biomimetic synthesis of silver nanoparticles from Streptomyces atrovirens and their potential anticancer activity against human breast cancer cells.

Silver nanoparticles (AgNPs) have been undeniable for its antimicrobial activity while its antitumour potential is still limited. Therefore, the present study focused on determining cytotoxic effects of AgNPs on Michigan cancer foundation-7 (MCF-7) breast cancer cells and its corresponding mechanism of cell death. Herein, the authors developed a bio-reduction method for AgNPs synthesis using actinomycetes isolated from marine soil sample. The isolated strain was identified by 16s ribotyping method and it was found to be Streptomyces atrovirens. Furthermore, the synthesised AgNPs were characterised by various bio-analytical techniques such as ultraviolet-visible spectrophotometer, atomic force microscopy, transmission electron microscopy, Fourier transform infra-red spectroscopy, and X-ray diffraction. Moreover, the results of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay reveals 44.51 µg of AgNPs to have profound inhibition of cancer cell growth; furthermore, the inhibition of MCF-7 breast cancer cell line was found to be dose dependent on treatment with AgNPs. Acridine orange and ethidium bromide double staining methods were performed for cell morphological analysis. The present results showed that biosynthesised AgNPs might be emerging alternative biomaterials for human breast cancer therapy.