[Immune complex formation during the passive immunization of mice infected with the tick-borne encephalitis virus]. / Obrazovanie immunnykh kompleksov pri passivnoi immunizatsii myshei, zarazhennykh virusom kleshchevogo éntsefalita.

The results of investigations, indicating that the development of the infectious process in experimental encephalitis is accompanied by the formation of immune complexes circulating in the blood and localized in the brain tissue, are presented. The intravenous injections of a homologous serum preparation into intact animals induces the appearance of a rather low level of circulating immune complexes, which precedes the elimination of antibodies. The intravenous injection of a specific serum preparation two days after the infection of the animals with tick-borne encephalitis virus is accompanied by the formation of immune complexes; the course of infection is not aggravated.

[Results of using the ELISA method in studying tick-borne encephalitis and rabies]. / Rezul'taty ispol'zovaniia metoda ELISA pri izuchenii kleshchevogo éntsefalita i beshenstva.

The results of using the indirect variant of ELISA for the study of serum samples from humans and white mice for the presence of antibodies to tick-borne encephalitis virus and rabies virus are presented. The high sensitivity and specificity of this method have been confirmed.

[Ultrastructural changes in the CNS of monkeys with the chronic form of tick-borne encephalitis]. / Ul'trastrukturnye izmeneniia v TsNS obez'ian pri khronicheskoi forme kleshchevogo éntsefalita.

The central nervous system of monkeys with chronic tick-borne encephalitis (TBE) shows the following ultrastructural alterations which differ from those in acute TBE: widespread destructive changes in neurons, astrocytes, oligodendrocytes, processes, myelin, vascular walls, severe edema of brain tissue with the signs of a so-called spongious degeneration and the absence of cell proliferation and perivascular cell infiltrations, glial nodules as well as circulatory disorders (hyperemia, stasis, hemorrhages). Few virions with or without and altered supercapsid membrane are detected in the neuronal cytoplasm or in the intercellular spaces; the signs of active virus reproduction in the cells are lacking. Pathologic process is of a degenerative but not of an inflammatory nature; the latter is typical for acute TBE.

[Possible use of a concentrated vaccine against tick-borne encephalitis for immunizing donors to obtain specific immunoglobulin]. / O vozmozhnosti primeneniia kontsentrirovannoi vaktsiny protiv kleshchevogo éntsefalita dlia immunizatsii donorov s tsel'iu polucheniia spetsificheskogo immunoglobulina.

The immunization of donors with dried, purified, inactivated and concentrated cell-culture tick-borne encephalitis vaccine, produced at the Institute of Poliomyelitis and Viral Encephalitides in Moscow, has been carried out with the aim of obtaining immune raw material for the production of specific donor immunoglobulin. The high immunogenic potency and low reactogenicity of the vaccine, introduced in 2 injections at an interval of 6 months, have been confirmed. Immunization in 2 injections has proved to be as effective as that achieved by 3 injections. The expediency of the immunization of regularly bled donors has been established: hemagglutinin titers in these donors have been found to be significantly higher than in a group of persons never bled before. The optimum time for taking blood has been determined: 3 weeks for regularly bled donors and 2 weeks for donors bled for the first time. Blood may be taken again 2 months after the first bleeding without booster immunization and 2 weeks after the third injection of the vaccine.

[Toxicity and radio-protective properties of pyridazine derivatives]. / Toksichnost' i radiozashchitnye svoistva proizvodnykh piridazina.

In experiments in irradiated (CBA X S57B1)F1 mice a study was made of toxicity and radioprotective efficiency of some pyridazine and pyridazine dione derivatives. Some of these substances were shown to have a moderate protective action when administered intraperitoneally. A correlation was revealed between the chemical structure and the radioprotective properties of the compounds.

[The effect of homologous gamma-globulin on nonspecific factors of antiviral immunity in prophylactic and therapeutic application]. / O vliianii gomologichnogo gamma-globulina na nespetsificheskie faktory protivovirusnogo immuniteta pri profilakticheskom i lechebnom ikh primenenii

Administration of homologous gamma-globulin for prophylactic purpose promoted an increase in the interferon-synthesizing activity of leukocytes. The therapeutic use of this preparation in tick-borne encephalitis produced no stimulating effect on this index.

[Primary pathological changes in cells infected with tick-borne encephalitis virus]. / Pervichnye patologicheskie izmeneniia v kletkakh, zarazhennykh virusom kleshchevogo éntsefalita

Synthesis and the content of RNA and protein in cells of the pig embryo kidneys infected with tick--borne encephalitis virus were studied by means of autoradiography and cytophotometry. Acceleration of synthesis and accumulation of RNA (in cellular structures) were estimated as over 125--200% of the initial level followed by a decrease in 12 hr when the virus release from the cells occurred. By the 2nd and 6--8th hr of infection (in the period of synthesis of early and late virus material), two peaks of protein contents over 123--131% were observed. The RNA and protein shifts are well correlated with the virus reproduction stages.