Biomethanol production from renewable resources: a sustainable approach.

The abundant availability of various kinds of biomass and their use as feedstock for the production of gaseous and liquid biofuels has been considered a viable, eco-friendly, and sustainable mode of energy generation. Gaseous fuels like biogas and liquid fuels, e.g., bioethanol, biodiesel, and biomethanol derived from biological sources, have been theorized to produce numerous industrially relevant organic compounds replacing the traditional practice of employing fossil fuels as a raw material. Among the biofuels explored, biomethanol has shown promising potential to be a future product addressing multifactorial issues concerning sustainable energy and associated process developments. The presented mini-review has explored the importance and application of biomethanol as a value-added product. The biomethanol production process was well reviewed by focusing on different thermochemical and biochemical conversion processes. Syngas and biogas have been acknowledged as potential resources for biomethanol synthesis. The emphasis on biochemical processes is laid on the principal metabolic pathways and enzymatic machinery involved or used by microbial physiology to convert feedstock into biomethanol under normal temperature and pressure conditions. The advantage of minimizing the cost of production by utilizing suggested modifications to the overall process of biomethanol production that involves metabolic and genetic engineering in microbial strains used in the production process has been delineated. The challenges that exist in our current knowledge domain, impeding large-scale commercial production potential of biomethanol at a cost-effective rate, and strategies to overcome them along with its future scenarios have also been pointed out.

Utilization of agricultural waste biomass and recycling toward circular bioeconomy.

The major global concern on energy is focused on conventional fossil resources. The burning of fossil fuels is an origin of greenhouse gas emissions resulting in the utmost threat to the environment and subsequently which leads to global climate changes. As far as sustainability is concerned, fuels and materials derived from organic or plant wastes overcome this downside establishing the solution to the fossil resource crisis. In this context, exploration of agricultural residue appears to be a suitable alternative of non-renewable resources to support the environmental feasibility and meet the high energy crisis. The use of agricultural waste as a feedstock for the biorefinery approach emerges to be an eco-friendly process for the production of biofuel and value-added chemicals, intensifying energy security. Therefore, a prospective choice of this renewable biomass for the synthesis of green fuel and other green biochemicals comes up with a favorable outcome in terms of cost-effectiveness and sustainability. Exploiting different agricultural biomass and exploring various biomass conversion techniques, biorefinery generates bioenergy in a strategic way which eventually fits in a circular bioeconomy. Sources and production of agricultural waste are critically explained in this paper, which provides a path for further value addition by various technologies. Biorefinery solutions, along with a life cycle assessment of agricultural waste biomass toward a wide array of value-added products aiding the bioeconomy, are summarized in this paper.

Effective hydrolysis for waste plant biomass impacts sustainable fuel and reduced air pollution generation: A comprehensive review.

Among various natural biowastes availability in the environment, agricultural residues showed great impacts. It is due to huge availability and cheap carbon source, creating big challenges for their utility and systematic reduction. Objective of this review is to address the waste biomass availability and huge quantities issues and also put effort to minimize this nutrient load via biotransforming into value-added products. Different wastes (organic/inorganic) generation with their negative issues are due to numbers of developmental and social activities, reported. Currently, various efforts are found for these wastes minimization via generation of different types of value-added products (biogas, bioH2, alcoholic fuel, organic acids and others products) and these wastes in municipal cities are also reported with production of advanced biofuels as promising outcomes. For hydrolysis of complex organic resources including lignocellulosic biomasses, physicochemical, structural or compositional changes are needed that aid in conversion into sugar and organic compounds such as biofuels. So, efficient and effective pretreatment processes selection (physical, biological, chemical or combined one) is critical to achieve these hydrolysis goals and resultant cellulose or hemicellulose components can be accessible by biological catalysis. These can achieve final hydrolysis and fermentative or monomer sugars. And later, synthesis of fuels or value-added products during microbial fermentation or biotransformation processes can be achieved. This review discusses pretreatment techniques for improved hydrolysis for fermentative sugar with emphasis on reduced quantities of toxic compounds (furfural compound) in hydrolyzed biomasses. Minimum deterioration fuel economy also reported with production of different bioproducts including biofuels. Additionally, impacts of toxic products and gasses emission are also discussed with their minimization.

Microbial fuel cells for waste nutrients minimization: Recent process technologies and inputs of electrochemical active microbial system.

Still, in the current period, non-renewable energy sources have contributed to fulfill the current energy requirement and it causes the deficit of available stocks with emission of pollutant byproducts components. In recent years, efforts were made to harness the sustainable/ renewable fuels from renewable sources/ waste, complex organic matters including biomass at global level. Researchers have put attention on harnessing energy from wastewater and MFCs systems. This MFC approach utilizes the wastewater as feed/ carbon sources to generate bioenergy such as bio- hydrogen and electricity in a sustainable and efficient way via completing the wastewater treatment. This approach consists of anode and cathode electrodes with proton exchange membrane (PEM). Microbial fuel cells are utilized with other effective approaches (anaerobic digestion/ fermentation, local demand-based-MFC approach) that are applied to utilize biowastes for microbial cell growth and electron emission. This review discusses the recent and technologically advanced approach such as microbial fuel cells (MFCs). MFCs can apply to fulfill the increasing trends of global energy demand, found with more utilization in home appliances in urban areas, industrial tasks and transportation sectors. Last few decades, there have been recent modifications in MFCs configurations and design that have improved performance of MFCs. Recent design and configuration of MFCs have shown the removal of maximum chemical oxygen demand (up to 94-97%) with generation maximum current output (up to 5.23 mA) from wastewater. This review presents a comprehensive collection of recent information about technologies and design of MFCs with implementation of microbial electrogene with development of wastewater treatment having sustainable fuel/ energy generation at an effective level.

Exploring the structural aspects and therapeutic perspectives of cyanobacterial phycobiliproteins.

Phycobiliproteins (PBPs) of cyanobacteria and algae possess unique light harvesting capacity which expand the photosynthetically active region (PAR) and allow them to thrive in extreme niches where higher plants cannot. PBPs of cyanobacteria/algae vary in abundance, types, amino acid composition and in structure as a function of species and the habitat that they grow in. In the present review, the key aspects of structure, stability, and spectral properties of PBPs, and their correlation with ecological niche of cyanobacteria are discussed. Besides their role in light-harvesting, PBPs possess antioxidant, anti-aging, neuroprotective, hepatoprotective and anti-inflammatory properties, which can be used in therapeutics. Recent developments in therapeutic applications of PBPs are reviewed with special focus on 'route of PBPs administration' and 'therapeutic potential of PBP-derived peptide and chromophores'.

Human Gut Microbiota and Mental Health: Advancements and Challenges in Microbe-Based Therapeutic Interventions.

Gut microbes play prime role in human health and have shown to exert their influence on various physiological responses including neurological functions. Growing evidences in recent years have indicated a key role of gut microbiota in contributing to mental health. The connection between gut and brain is modulated by microbes via neural, neuroendocrinal and metabolic pathways that are mediated through various neurotransmitters and their precursors, hormones, cytokines and bioactive metabolites. Impaired functioning of this connection can lead to manifestation of mental disorders. Around 1 billion of the world population is reported to suffer from emotional, psychological and neurological imbalances, substance use disorders and cognitive, psychosocial and intellectual disabilities. Thus, it becomes imperative to understand the role of gut microbes in mental disorders. Since variations occur in the conditions associated with different mental disorders and some of them have overlapping symptoms, it becomes important to have a holistic understanding of gut dysbiosis in these disorders. In this review, we consolidate the recent data on alterations in the gut microbes and its consequences in various neurological, psychological and neurodegenerative disorders. Further, considering these evidences, several studies have been undertaken to specifically target the gut microbiota through different therapeutic interventions including administration of live microbes (psychobiotics) to treat mental health disorders and/or their symptoms. We review these studies and propose that an integrative and personalized approach, where combinations of microbe-based therapeutic interventions to modulate gut microbes and in-use psychological treatment practices can be integrated and based on patient's gut microbiome can be potentially adopted for effective treatment of the mental disorders.

Aquatic Plants, Landoltia punctata, and Azolla filiculoides as Bio-Converters of Wastewater to Biofuel.

The aquatic plants, Azolla filiculoides, and Landoltia punctate, were used as complementing phytoremediators of wastewater containing high levels of phosphate, which simulates the effluents from textile, dyeing, and laundry detergent industries. Their complementarities are based on differences in capacities to uptake nitrogen and phosphate components from wastewater. Sequential treatment by L. punctata followed by A. filiculoides led to complete removal of NH4, NO3, and up to 93% reduction of PO4. In experiments where L. punctata treatment was followed by fresh L. punctata, PO4 concentration was reduced by 65%. The toxicity of wastewater assessed by shrimps, Paratya australiensis, showed a four-fold reduction of their mortality (LC50 value) after treatment. Collected dry biomass was used as an alternative carbon source for heterotrophic marine protists, thraustochytrids, which produced up to 35% dry weight of lipids rich in palmitic acid (50% of total fatty acids), the key fatty acid for biodiesel production. The fermentation of treated L. punctata biomass by Enterobacter cloacae yielded up to 2.14 mol H2/mole of reduced sugar, which is comparable with leading terrestrial feedstocks. A. filiculoides and L. punctata can be used as a new generation of feedstock, which can treat different types of wastewater and represent renewable and sustainable feedstock for bioenergy production.

Carbon and hydrogen isotope analysis of parathion for characterizing its natural attenuation by hydrolysis at a contaminated site.

The applicability of compound-specific isotope analysis (CSIA) for assessing in situ hydrolysis of parathion was investigated in a contaminated aquifer at a former pesticide wastes landfill site. Stable isotope analysis of parathion extracted from groundwater taken from different monitoring wells revealed a maximum enrichment in carbon isotope ratio of +4.9‰ compared to the source of parathion, providing evidence that in situ hydrolysis took place. Calculations based on the Rayleigh-equation approach indicated that the natural attenuation of parathion was up to 8.6% by hydrolysis under neutral and acidic conditions. In degradation experiments with aerobic and anaerobic parathion-degrading microbes, no carbon and hydrogen isotope fractionation of parathion were observed. For the first time, CSIA has been applied for the exclusive assessment of the hydrolysis of phosphorothioate-containing organophosphorus pesticides at a contaminated field site.

Polarized electrode enhances biological direct interspecies electron transfer for methane production in upflow anaerobic bioelectrochemical reactor.

The influence of polarized electrodes on the methane production, which depends on the sludge concentration, was investigated in upflow anaerobic bioelectrochemical (UABE) reactor. When the polarized electrode was placed in the bottom zone with a high sludge concentration, the methane production was 5.34 L/L.d, which was 53% higher than upflow anaerobic sludge blanket (UASB) reactor. However, the methane production was reduced to 4.34 L/L.d by placing the electrode in the upper zone of the UABE reactor with lower sludge concentration. In the UABE reactor, the methane production was mainly improved by the enhanced biological direct interspecies electron transfer (bDIET) pathway, and the methane production via the electrode was a minor fraction of less than 4% of total methane production. The polarized electrodes that placed in the bottom zone with a high sludge concentration enhance the bDIET for methane production in the UABE reactor and greatly improve the methane production.

Applications of microalgal biofilms for wastewater treatment and bioenergy production.

BACKGROUND: Microalgae have shown clear advantages for the production of biofuels compared with energy crops. Apart from their high growth rates and substantial lipid/triacylglycerol yields, microalgae can grow in wastewaters (animal, municipal and mining wastewaters) efficiently removing their primary nutrients (C, N, and P), heavy metals and micropollutants, and they do not compete with crops for arable lands. However, fundamental barriers to the industrial application of microalgae for biofuel production still include high costs of removing the algae from the water and the water from the algae which can account for up to 30-40% of the total cost of biodiesel production. Algal biofilms are becoming increasingly popular as a strategy for the concentration of microalgae, making harvesting/dewatering easier and cheaper. RESULTS: We have isolated and characterized a number of natural microalgal biofilms from freshwater, saline lakes and marine habitats. Structurally, these biofilms represent complex consortia of unicellular and multicellular, photosynthetic and heterotrophic inhabitants, such as cyanobacteria, microalgae, diatoms, bacteria, and fungi. Biofilm #52 was used as feedstock for bioenergy production. Dark fermentation of its biomass by Enterobacter cloacae DT-1 led to the production of 2.4 mol of H2/mol of reduced sugar. The levels and compositions of saturated, monosaturated and polyunsaturated fatty acids in Biofilm #52 were target-wise modified through the promotion of the growth of selected individual photosynthetic inhabitants. Photosynthetic components isolated from different biofilms were used for tailoring of novel biofilms designed for (i) treatment of specific types of wastewaters, such as reverse osmosis concentrate, (ii) compositions of total fatty acids with a new degree of unsaturation and (iii) bio-flocculation and concentration of commercial microalgal cells. Treatment of different types of wastewaters with biofilms showed a reduction in the concentrations of key nutrients, such as phosphates, ammonia, nitrates, selenium and heavy metals. CONCLUSIONS: This multidisciplinary study showed the new potential of natural biofilms, their individual photosynthetic inhabitants and assembled new algal/cyanobacterial biofilms as the next generation of bioenergy feedstocks which can grow using wastewaters as a cheap source of key nutrients.

Bioelectrochemical enhancement of direct interspecies electron transfer in upflow anaerobic reactor with effluent recirculation for acidic distillery wastewater.

Methane production in the upflow anaerobic bioelectrochemical reactor (UABE) treating acidic distillery wastewater was compared to the upflow anaerobic sludge blanket reactor (UASB), and the electron transfer pathways for methane production were also evaluated in the effluent recirculation. The methane productions from reactors were influenced by the low pH of influent wastewater. However, the methane production rate and yield of the UABE were 2.08L/L.d and 320mL/g CODr, which were higher than the UASB. The effluent recirculation containing alkalinity neutralized the acidic influent and increased the upflow velocity in both reactors, and improved the direct interspecies electron transfer more in the UABE. When the effluent recirculation ratio was 3.0 in the UABE, the methane production rate and yield were reached up to 3.88L/L.d and 501.0mL/g CODr, respectively. The UABE requires electrode installation and electrical energy for operation, but the benefits from increased methane production are much higher.

Characterization of a Novel Polymeric Bioflocculant Produced from Bacterial Utilization of n-Hexadecane and Its Application in Removal of Heavy Metals.

A novel polymeric bioflocculant was produced by a bacterium utilizing degradation of n-hexadecane as the energy source. The bioflocculant was produced with a bioflocculating activity of 87.8%. The hydrocarbon degradation was confirmed by gas chromatography-mass spectrometry analysis and was further supported with contact angle measurements for the changes in hydrophobic nature of the culture medium. A specific aerobic degradation pathway followed by the bacterium during the bioflocculant production and hydrocarbon utilization process has been proposed. FT-IR, SEM-EDX, LC/MS, and 1H NMR measurements indicated the presence of carbohydrates and proteins as the major components of the bioflocculant. The bioflocculant was characterized for its carbohydrate monomer constituents and its practical applicability was established for removing the heavy metals (Ni2+, Zn2+, Cd2+, Cu2+, and Pb2+) from aqueous solutions at concentrations of 1-50 mg L-1. The highest activity of the bioflocculant was observed with Ni2+ with 79.29 ± 0.12% bioflocculation efficiency.

Effect of electrode surface properties on enhanced electron transfer activity in microbial fuel cells.

The influence of electrode surface chemistry over biofilm growth was evaluated for photo-bioelectrocatalytic fuel cell. A consortium of photosynthetic bacteria was grown onto different electrodes designed with polyethylenimine (PEI) and multiwall carbon nanotubes as hydrophilic and hydrophobic modifier, respectively. The designed electrodes were loaded with 0.08, 0.17, and 0.33 µg/cm2 of PEI to change the hydrophilicity. However, 0.56, 0.72, and 0.83 mg/cm2 of multiwall carbon nanotubes were used to alter the hydrophobicity of the electrodes. The surface chemistry of electrode and bio-interaction was evaluated as a function of contact angle and biofilm formation. The results were compared with those obtained with a carbon paper electrode. The contact angle on the untreated electrode (carbon paper) was 118°, whereas for hydrophobic and hydrophilic electrodes, the maximum and minimum contact angles were 170° and 0°, respectively. Interestingly, the maximum biofilm growth (0.2275 g, wet basis) was observed on highly hydrophobic surface; however, the maximum electrochemical performance (246 mV) was shown by the most hydrophilic electrode surface. PEI-based electrode with good biofilm formation showed comparatively higher electrogenic activity.

Aquatic plant Azolla as the universal feedstock for biofuel production.

BACKGROUND: The quest for sustainable production of renewable and cheap biofuels has triggered an intensive search for domestication of the next generation of bioenergy crops. Aquatic plants which can rapidly colonize wetlands are attracting attention because of their ability to grow in wastewaters and produce large amounts of biomass. Representatives of Azolla species are some of the fastest growing plants, producing substantial biomass when growing in contaminated water and natural ecosystems. Together with their evolutional symbiont, the cyanobacterium Anabaena azollae, Azolla biomass has a unique chemical composition accumulating in each leaf including three major types of bioenergy molecules: cellulose/hemicellulose, starch and lipids, resembling combinations of terrestrial bioenergy crops and microalgae. RESULTS: The growth of Azolla filiculoides in synthetic wastewater led up to 25, 69, 24 and 40 % reduction of NH4-N, NO3-N, PO4-P and selenium, respectively, after 5 days of treatment. This led to a 2.6-fold reduction in toxicity of the treated wastewater to shrimps, common inhabitants of wetlands. Two Azolla species, Azolla filiculoides and Azolla pinnata, were used as feedstock for the production of a range of functional hydrocarbons through hydrothermal liquefaction, bio-hydrogen and bio-ethanol. Given the high annual productivity of Azolla, hydrothermal liquefaction can lead to the theoretical production of 20.2 t/ha-year of bio-oil and 48 t/ha-year of bio-char. The ethanol production from Azolla filiculoides, 11.7 × 103 L/ha-year, is close to that from corn stover (13.3 × 103 L/ha-year), but higher than from miscanthus (2.3 × 103 L/ha-year) and woody plants, such as willow (0.3 × 103 L/ha-year) and poplar (1.3 × 103 L/ha-year). With a high C/N ratio, fermentation of Azolla biomass generates 2.2 mol/mol glucose/xylose of hydrogen, making this species a competitive feedstock for hydrogen production compared with other bioenergy crops. CONCLUSIONS: The high productivity, the ability to grow on wastewaters and unique chemical composition make Azolla species the most attractive, sustainable and universal feedstock for low cost, low energy demanding, near zero maintenance system for the production of a wide spectrum of renewable biofuels.

Purification and characterization of exopolysaccharide bioflocculant produced by heavy metal resistant Achromobacter xylosoxidans.

Optimization of process parameters enhanced bioflocculating activity of 'Achromobacter xylosoxidans strain TERI L1' from 75% to 83.3% in absence of heavy metals, which decreased to 73% in presence of multi-metals. 'TERI L1' could adsorb 90% of multi-metals when grown in presence of 1250 mg L(-1) Zn, 2 mg L(-1) Cd, 30 mg L(-1) Pb, 200 mg L(-1) Ni and 90 mg L(-1) Cu and could adsorb 1100 mg L(-1) of Pb when grown in presence of 1500 ppm lead nitrate. The bioflocculant was purified and characterized. Bioflocculant yield was 5 g L(-1). Fourier transform infrared spectrum indicated presence of carboxyl, hydroxyl, amino groups, typical of glycoprotein. Spectroscopic analysis of bioflocculant by nuclear magnetic resonance revealed that it is a glycoprotein. LC-MS analysis confirmed the bioflocculant as a carbohydrate hetero polymer. Bioflocculant was composed of 75% total sugar with 72.9% neutral sugar and 11.5% protein. Scanning Electron Micrography revealed effective flocculation of kaolin clay by purified exopolysaccharide bioflocculant.

Implication of composite electrode on the functioning of photo-bioelectrocatalytic fuel cell operated with heterotrophic-anoxygenic condition.

Electrode materials play a vital role in biofilm formation and electron conduction for efficient functioning of fuel cells. In the present study, graphite polymer composite electrode (GPF) was evaluated as anode for photo-bioelectrocatalytic fuel cell (PhFC; biophotovoltaic system) and compared with much studied graphite electrode (Gc) with photosynthetic bacteria as biocatalyst under anoxygenic condition. The electrogenic activity noticed in GPF (584mV; 2.67mA) was slightly lower than Gc (604mV; 2.92mA; OL2/HRT2). Consequently, COD removal observed by GPF (87.3%) was lower than Gc (91.8%). The increase in bacterial chlorophyll pigment showed a positive influence on electrogenic activity for both the electrodes. The polarization resistance (OL2 and HRT2 condition) was significantly higher for GPF (330Ω) as compared to Gc (110Ω). It is interesting to note that the performance of GPF is slightly lower than Gc based PhFC. The findings have opened avenues for composite materials for PhFC.

Bioflocculant production and biosorption of zinc and lead by a novel bacterial species, Achromobacter sp. TERI-IASST N, isolated from oil refinery waste.

A bioflocculant-producing bacterial isolate designated as 'TERI-IASST N' was isolated from activated sludge samples collected from an oil refinery. This isolate demonstrated maximum bioflocculation activity (74%) from glucose among 15 different bioflocculant-producing bacterial strains isolated from the sludge samples and identified as Achromobacter sp. based on 16S rRNA gene sequence. Optimization of pH and supplementation of urea as nitrogen source in the production medium enhanced the flocculation activity of strain TERI-IASST N to 84% (at pH 6). This strain revealed maximum flocculation activity (90%) from sucrose compared to the flocculation activity observed from other carbon sources as investigated (glucose, lactose, fructose, maltose and starch). Ca(2+) served as the suitable divalent cation for maximum bioflocculation activity of TERI-IASST strain N. Maximum flocculation activity was observed at optimum C/N ratio of 1. Flocculation activity of this strain decreased to 75% in the presence of heavy metals; Zn, Pb, Ni, Cu and Cd. In addition strain N revealed considerable biosorption of Zn (430mgL(-1)) and Pb (30mgL(-1)). Bioflocculant yield of strain N was 10.5gL(-1). Fourier transform infrared spectrum indicated the presence of carboxyl, hydroxyl, and amino groups, typical of glycoprotein. Spectroscopic analysis of bioflocculant by nuclear magnetic resonance revealed that it is a glycoprotein, consisting of 57% total sugar and 13% protein.

Isolation of a lead tolerant novel bacterial species, Achromobacter sp. TL-3: assessment of bioflocculant activity.

Lead is one of the four heavy metals that has a profound damaging effects on human health. In the recent past there has been an increasing global concern for development of sustainable bioremediation technologies for detoxification of lead contaminant. Present investigation highlights for lead biosorption by a newly isolated novel bacterial species; Achromobacter sp. TL-3 strain, isolated from activated sludge samples contaminated with heavy metals (collected from oil refinery, Assam, North-East India). For isolation of lead tolerant bacteria, sludge samples were enriched into Luria Broth medium supplemented separately with a range of lead nitrate; 250, 500, 750, 1000, 1250 and 1500 ppm respectively. The bacterial consortium that could tolerate 1500 ppm of lead nitrate was selected further for purification of lead tolerant bacterial isolates. Purified lead tolerant bacterial isolates were then eventually inoculated into production medium supplemented with ethanol and glycerol as carbon and energy source to investigate for bioflocculant production. Bioflocculant production was estimated by monitoring the potential of lead tolerant bacterial isolate to flocculate Kaolin clay in presence of 1% CaCl2. Compared to other isolates, TL-3 isolate demonstrated for maximum bioflocculant activity of 95% and thus was identified based on 16S rRNA gene sequence analysis. TL3 isolate revealed maximum homology (98%) with Achromobacter sp. and thus designated as Achromobacter sp. TL-3. Bioflocculant activity of TL-3 isolate was correlated with the change in pH and growth. Achromobacter sp. TL-3 has significant potential for lead biosorption and can be effectively employed for detoxification of lead contaminated waste effluents/waste waters.

Draft genome sequence of Arthrospira platensis C1 (PCC9438).

Arthrospira platensis is a cyanobacterium that is extensively cultivated outdoors on a large commercial scale for consumption as a food for humans and animals. It can be grown in monoculture under highly alkaline conditions, making it attractive for industrial production. Here we describe the complete genome sequence of A. platensis C1 strain and its annotation. The A. platensis C1 genome contains 6,089,210 bp including 6,108 protein-coding genes and 45 RNA genes, and no plasmids. The genome information has been used for further comparative analysis, particularly of metabolic pathways, photosynthetic efficiency and barriers to gene transfer.

Truncation mutants highlight a critical role for the N- and C-termini of the Spirulina Delta(6) desaturase in determining regioselectivity.

The results of our previous study on heterologous expression in Escherichia coli of the gene desD, which encodes Spirulina Delta(6) desaturase, showed that co-expression with an immediate electron donor-either cytochrome b ( 5 ) or ferredoxin-was required for the production of GLA (gamma-linolenic acid), the product of the reaction catalyzed by Delta(6) desaturase. Since a system for stable transformation of Spirulina is not available, studies concerning Spirulina-enzyme characterization have been carried out in heterologous hosts. In this present study, the focus is on the role of the enzyme's N- and C-termini, which are possibly located in the cytoplasmic phase. Truncated enzymes were expressed in E. coli by employing the pTrcHisA expression system. The truncation of the N- and C-terminus by 10 (N10 and C10) and 30 (N30 and C30) amino acids, respectively, altered the enzyme's regioselective mode from one that measures from a preexisting double bond to that measuring from the methyl end of the substrate.