[Molecular-genetic analysis of natural and stimulated ovulation impairment].

The influence of FMR1, INHalpha1, NAT2, GSTT1 and GSTM1 genes on ovarian function, and their association with POF and "poor response" to exogenous GT after ovulation stimulation were investigated. The carriers of Ala257Thr transition predominated in the studied "poor responders" group. This transition combined with intermediate alleles of FMR1 gene was observed in 1.6% POF patients and 2.5% persons from "poor responders" group but in nobody of the control group. The frequency of deletion in GSTM1 gene in "poor responders" group was significantly higher (p = 0,01) than in normal ovulatory control group. The frequency of Ser680Ser-Ala307Ala polymorphic genotype (22.2%) in "poor responders" group was significantly higher (p = 0.028) than in normal-ovulatory control group (7.7%). The daily dosage of GT in intermediate alleles of FMR1 gene carriers as well in patients with "slow acetylation" NAT2 genotype was significantly higher in comparison to patients without intermediate alleles and patients with "quick acetylation" NAT2 genotype. Quantity of oocytes after ovulation stimulation in women with INHa1 gene Ala257Thr transition was significantly decreased in comparison to patients without such mutation. Further investigations of these genes can play a major role in POF studying and modulation of ovarian response to exogenous GT.

[The use of DNA analysis for diagnostics of hereditary premature ovarian failure].

Methods of DNA-analysis of 769G --> A mutations in INHalpha1 gene and CGG-repeats polymorphism in FMRI gene have been developed for creating test-systems for genetically caused forms of premature ovarian failure (POF) diagnostics. The frequency of 769G --> A mutation among women population in Ukraine was established and, by preliminary calculations, makes up 2.8%. Results of analysis of CGG-repeats numbers in FMRI gene in the group of 215 women (oocyte donors) revealed five persons with CGG-repeats numbers, that exceeds the normal one (42 copies). Thus the frequency of persons with allels with high risk of premutation in FMRI gene is 2.3%. The results of our research confirm the actuality of genetic tests of mutations in INHalpha1 and FMR1 genes among the women of reproductive age with the purpose of POF prognosis and prevention the birth of children with fragile X syndrome.

[Experience in the use of vaginal and intracervical enzaprost suppositories in preparation for labor]. / Opyt primeneniia vlagalishchnykh i intratservikal'nykh svechei s énzaprostom dlia dorodovoi podgotovki.

To improve the efficacy of prelabor treatment of pregnant women with some obstetrical abnormalities, such as over-term pregnancy, AB0- and Rh-isosensitization, anemia, gestosis of the second half of pregnancy, vaginal and intracervical suppositories containing 0.1 mg of enzaprost were administered to 102 women for 1-5 days. In 30 (29.4%) cases such treatment induced spontaneous labor. In 64 (62.7%) women labor was induced by deoxy-amino oxytocin, and in 8 (7.8%) women this latter drug had to be administered repeatedly to induce labor. The babies born to the women under observation had the Apgar score of 6-10, 92 (90.2%) of them had the score of 8-10. No stillbirths or early infant mortality cases were recorded. Therefore prelabor treatment with vaginal or intracervical suppositories with enzaprost is effective and safe for both mother and newborn.

[Hysteroscopic semeiotics of cancer of the uterine body]. / Gisteroskopicheskaia semiotika raka tela matki.

Hysteroscopic features of preinvasive and invasive uterine cancer as well as the adjacent endometrium were studied in 103 patients. The following types of early cancer were identified with regard to pattern of growth: a single lesion, multiple lesions and diffuse involvement of the endometrium. On the basis of hysteroscopic signs, tumors were classified into polypoid, plaque-shaped, cystic mucin-producing forms and tumors with predominant atypical vasculature. Clinically apparent endometrial tumors were hysteroscopically classified as circumscribed and diffuse. The former were further subdivided into nodular, polypoid, papillary and cystic mucin-producing patterns. The credibility of hysteroscopic diagnosis was 81.8% for preinvasive and 100%--for invasive uterine carcinoma. Hysteroscopy proved instrumental in uterine cancer diagnosis, particularly, for the preinvasive form since it allows aiming sampling for histological examination to be carried out.