RESUMO
Mongolia had no reported cases of capripoxvirus disease from 1977 until an outbreak of sheeppox in 2006-2007 and then goatpox in 2008. The two outbreaks occurred in geographically distant areas of Mongolia and, most strikingly, were highly species-specific. The 2006-2007 sheeppox outbreak affected no goats and the 2008 goatpox outbreak affected no sheep despite communal herding. The diseases were diagnosed using the polymerase chain reaction and virus neutralisation test. The P32 gene of the Mongolian sheeppox and goatpox viruses from the recent outbreaks were sequenced and compared with an archived 1967 strain of Goatpox virus from Mongolia. The P32 gene of the 2006-2007 Mongolian Sheeppox virus strain was identical to previously published sheeppox strains. The P32 gene of the 2008 Mongolian Goatpox virus strain was identical to the gene from virus isolated from recent goatpox outbreaks in China and Vietnam. The archived Mongolian Goatpox virus strain was unique.
Assuntos
Surtos de Doenças/veterinária , Doenças das Cabras/virologia , Infecções por Poxviridae/veterinária , Doenças dos Ovinos/epidemiologia , Doenças dos Ovinos/virologia , Animais , Capripoxvirus/classificação , Capripoxvirus/genética , Capripoxvirus/isolamento & purificação , China , Cabras , Mongólia , Filogenia , Infecções por Poxviridae/diagnóstico , Infecções por Poxviridae/epidemiologia , Ovinos , Doenças dos Ovinos/diagnóstico , Especificidade da Espécie , VietnãRESUMO
Background:Echinococcosis is from animals to humans and cause cestode zoonoses. Genetic variations within of echonoccocus and their genotypes may cause a disease as well as can indicate transmission dynamics to human and pets. At present, there are no available data for the typing of echinococcosis isolated in MongoliaMaterials and Methods:A total of 50 human hydatid samples from collected from State Centre on Maternal and Child Health, Oncology Centre of Mongolia. All samples were examined by PCR using cox1. The PCR products with a molecular size of 578 bp were amplified from human hydatid samples. Also we used RFLP method.Results:Genotype and strains of E. multilocularis and Е. granulosus were identified by RFLP. PCR products were digested using Ssp I, Hind III, Bgl II endonucleases. PCR products were digested by Ssp I endonuclease we found E. multilocularis. PCR products were digested by Bgl II endonuclease. Two major bands were seen in human hydatid sample. The bands have molecular weight of 420 and 158 bp respectively. It was infected by E. granulosus G6. Digestion with Hind III revealed two major bands within samples from human hydatids. These bands have molecular weight of 168, 410 bp respectively. These samples were infected by E. granulosus G1. Most of E. granulosus materials obtained from human patients by surgery confirmed the presence of sheep strain G1 (Bowles and McManus, 1993 a & c). In 24 cases of human hydatid echinococcosis in Mongolia sheep strain was found to be infective to humans.Conclusions:1. Echinococcosis caused by E. granulosus, E. multilocularis in human.2. G1, G6 genotypes of E. granulosus found in human hydatids.
Assuntos
Glaucoma de Ângulo Aberto/diagnóstico , Adulto , Feminino , Glaucoma de Ângulo Aberto/patologia , Humanos , Hiperopia/diagnóstico , Iris/patologia , Cápsula do Cristalino/patologia , Masculino , Pessoa de Meia-Idade , Miopia/diagnóstico , Epitélio Pigmentado Ocular/patologia , Malha Trabecular/patologiaRESUMO
We saw an unusual case of spontaneous Hemophilus influenzae purulent pericarditis in an adult. Counterimmunoelectrophoresis (CIE) of the pericardial fluid may be used to make an early, accurate diagnosis. Treatment should include pericardectomy and immediate use of both ampicillin sodium and chloramphenicol sodium succinate, until the sensitivity of the organism is known.
