Exploring porcine kidney explants as a model for the study of nephrotoxins and the therapeutic potential of phytic acid.

The use of in vivo models to assess nephrotoxicity has faced ethical limitations. A viable alternative is the ex vivo model that combines the 3 R principles with the preservation of tissue histology. Here, we established a gentamicin nephrotoxicity model using pigs` kidney explants and investigated the effect of phytic acid (IP6) against gentamicin- induced nephrotoxicity. A total of 360 kidney explants were divided into control, gentamicin (10 mM), IP6 (5 mM), and gentamicin+IP6 groups. The activity of gammaglutamyltransferase (GGT), creatinine levels, histological assessment, oxidative stress, and inflammatory cytokine expression were analyzed. Exposure to gentamicin induced an increase in GGT activity, creatinine levels, lesion score, lipoperoxidation and IL-8 expression. Explants exposed to IP6 remained like the control. The addition of IP6 to gentamicin prevented tissue damage, increasing the antioxidant status and gene expression of IL-10. This model proved to be an adequate experimental approach for identifying nephrotoxins and potential products to modulate the toxicity.

Pneumonia due to Talaromyces marneffei in a Dog from Southern Brazil with Concomitant Canine Distemper Virus Infection.

The pathological and molecular findings associated with Talaromyces marneffei-induced pneumonia with concomitant infection by canine distemper virus (CDV) are described in a dog. The principal pathological alteration occurred in the lungs. Histopathology confirmed multifocal granulomatous pneumonia associated with numerous intralesional and intracellular septate fission cells consistent with T. marneffei. A molecular assay designed to amplify a partial fragment of the 18S rRNA gene of T. marneffei provided positive results from two fungal cultures derived from the lung. Sequencing and phylogenetic analyses confirmed the results of polymerase chain reaction (PCR). Furthermore, antigens of the CDV N protein were identified within the bronchial epithelium by immunohistochemistry and a PCR assay amplified the CDV N gene from hepatic and pulmonary fragments. Collectively, the pathological and molecular techniques confirmed a diagnosis of T. marneffei-induced pneumonia with concomitant infection by CDV. These findings represent the first description of pulmonary penicilliosis in the dog and extend the geographical niche of this emerging infectious pathogen. In this case, infection by CDV may have induced immunosuppression, which facilitated the development of pulmonary penicilliosis.

Manejo nutricional de cães e gatos domiciliados no estado do Espírito Santo - Brasil / Feeding patterns of dogs and cats in Espírito Santo - Brazil

Obtiveram-se informações sobre o manejo nutricional adotado para cães e gatos domiciliados, avaliou-se o grau de conhecimento dos proprietários e correlacionaram-se esses dados com fatores socioeconômicos dos entrevistados. Um questionário foi respondido por 496 proprietários de cães e/ou gatos. Verificou-se que os proprietários reconhecem que a alimentação está diretamente relacionada com a saúde animal e se preocupam com a qualidade do alimento fornecido. A ração seca é considerada a melhor opção para a alimentação de cães e gatos e é o tipo de alimento mais utilizado pelos proprietários. Os cães são alimentados preferencialmente duas vezes ao dia, e os gatos recebem alimento à vontade. Os proprietários buscam informações sobre a alimentação dos animais principalmente com veterinários ou estudantes de veterinária e lojas agropecuárias ou pet shops. Em geral, os proprietários de maior escolaridade têm preferência pela ração seca. O custo com a alimentação dos animais é considerado alto, especialmente para os proprietários que fornecem rações terapêuticas. Dessa forma, não são todos que podem arcar com esse custo. Observou-se que os proprietários que fornecem esse tipo de ração são os que apresentam maior poder aquisitivo. O tipo de alimento fornecido ao animal, ração ou comida caseira, não é influenciado pelo ambiente no qual o proprietário reside (rural ou urbano).

In this study, further information on nutritional management performed by dog and cat owners was obtained, the level of owners' knowledge about pet nutrition was assessed, and some aspects with socioeconomic background of respondents were correlated. A survey was conducted with 496 dog and cat owners. According to the results, owners recognize that nutrition is closely related to animal health and they care about the quality of food provided. Commercial dry foods are considered the best option for feeding dogs and cats and it is the type of food used by most owners. Dogs are fed preferably twice a day and cats ad libitum. Owners seek information on pet nutrition especially with veterinarians or veterinary students and agricultural shops or pet shops. In general, owners with higher education have a preference for dry foods. The cost with pet food is considered high, especially for owners who provide therapeutic diets. Few owners can afford this cost. We found that owners who provide this type of diet are those with higher purchasing power. The type of diet given to pets, commercial or homemade food, is not influenced by the environment in which the owner resides (rural or urban).