RESUMO
Crude RNA isolated from Piptoporus betulinus and two fractions of it obtained after the chromatography contain single-stranded RNA. The crude RNA, but not its chromatographic fractions, are resistant to the pancreatic RNA-ase. The crude preparation of RNA from P. betulinus contains beside ribose, considerable amounts of glucose, galactose and mannose. The crude RNA from P. betulinus induces in the human fibroblasts interferon with specific activity of 400 units per mg of protein.
Assuntos
Basidiomycota/análise , Indutores de Interferon , Polyporaceae/análise , RNA/farmacologia , Replicação Viral/efeitos dos fármacos , Células Cultivadas , Relação Dose-Resposta a Droga , Humanos , Peso Molecular , RNA/análise , Ribonucleases/metabolismo , Simplexvirus/crescimento & desenvolvimento , Vaccinia virus/crescimento & desenvolvimentoRESUMO
Tebrofen inhibited the replication of western equine encephalomyelitis (WEE) virus in multicycle experiments in chick embryo cell cell cultures. The compound did not inhibit the replication of vaccinia and Newcastle disease viruses.
Assuntos
Antivirais , Compostos de Bifenilo/farmacologia , Vírus da Encefalite Equina do Oeste/efeitos dos fármacos , Animais , Células Cultivadas/efeitos dos fármacos , Embrião de Galinha , Vírus da Encefalite Equina do Oeste/crescimento & desenvolvimento , Vírus da Doença de Newcastle/efeitos dos fármacos , Bifenil Polibromatos , Resorcinóis/farmacologia , Vaccinia virus/efeitos dos fármacos , Replicação Viral/efeitos dos fármacosRESUMO
The effects of divalent cations (barium, cobalt, calcium, copper, magnesium, zinc and iron) on the interference of tick-borne encephalitis (TE) virus with vaccinia virus in Detroit-6 cell cultures were investigated. An increase in interference was shown in the presence of cobalt, zinc, copper, and magnesium. Barium and iron displayed a marked inhibitory action. But an increase in interference was not parallel with an increased production of interferon.
