Hemostasis in children with dysbacteriosis in chronic constipation.

The purpose of the study was to investigate hemostasis in children with dysbacteriosis disturbance in chronic constipation. The disturbance of factors in the inner mechanism of blood coagulation (VII, IX, XI, XII) in compensated chronic constipation was defined based on the reduction in colon bacillus levels. We observed hypocoagulation caused by the reduced activity of the prothrombin complex factors, disaggregate thrombocytopathy, and endotheliosis with fibrinolysis inhibition in subcompensated chronic colostasis with continuous reduction of colon bacillus levels and pathogenic microflora appearance. In decompensated colostasis there was an increase in pathogenic microorganisms and a continuous reduction of colon bacillus levels. In hemostasis there was a factor deficiency in inner (XII, XI, IX, VIII) and outer (II, V, VII, X) blood coagulation mechanisms. Fibrinolysis inhibition, endotheliosis development with thrombocyte aggregation, and microthrombosis formation were determined. Thus, in children with chronic constipation, there was a marked reduction in the amount of colon bacillus, which led to the reproduction of pathogenic bacteria. We also observed chronometric hypocoagulation with the inner (XII, XI, IX, VIII) and outer (II, V, VII, X) mechanisms of blood coagulation, at the base of which there is the deficiency of vitamin K-dependent factors (II, VII, IX, X) and a slightly marked disturbance in the final stage of coagulation. In thrombocyte vascular hemostasis, thrombocytopathy was observed with increased adenosine-5-diphosphate aggregation and the inhibition of the inner mechanism with fibrinolysis and endotheliosis.

The peculiar features of changing the hemostasis in children with chronic constipation.

Our purpose was to study the peculiarities of changing the hemostasis in children with chronic constipation. In the course of our investigation of hemostasis in children with chronic constipation, we found chronometric hypocoagulation with impairment of internal (XII, XI, IX, VIII) and external (II, V, VII, X) mechanisms of blood clotting, at the base of which is a deficit of vitamin K-dependent factors (II, VII, IX, X) and a slight impairment of the final stage of coagulation. In thrombocytovascular hemostasis, thrombocytopathy demonstrated increased adenosine-5-diphosphate aggregation and suppression of the internal route of fibrinolysis and endotheliosis. We must point out that all these changes lead to impairment of microcirculation in the form of thromboses, particularly in the submucous and mucous membrane vessels, which is confirmed by the morphologic findings. In the compensated form, chronometric hypocoagulation with a deficit of II, IX, and X factors is noted. In the subcompensated form, hypocoagulation increases, and the deficit of V and VII factors is in part the cause. Endotheliosis and the increase of the thrombogenesis dynamics are noted in the thrombocytovascular section. In the decompensated form, there is marked hypocoagulation. Low activity of antithrombin III leads to high content of fibrinmonomeasured complexes in combination with reduction of the thrombocytes quantity and to hypercoagulation. The present changes are confirmed by the morphologic research methods of studying the vascular channel of the large intestine wall, where organized thrombi and a marked plethora of neutrophil tissue infiltration are often revealed.

[A method of immunoenzyme analysis for determining human histocompatibility antigens]. / Metod immunofermentnogo analiza dlia opredeleniia antigenov gistosovmestimosti cheloveka.

Enzyme immunoassay (EIA) has been applied to measurements of human histocompatibility (HLA) antigens (antibodies). The optimal concentration of the antigen immobilized on solid phase and the optimal dilutions of HLA sera have been found. The assays have been performed with the peripheral blood lymphocytes and platelets from donors with a known HLA phenotype. Horseradish peroxidase (Sigma, Rz-2.75) has been employed as the enzymic label, polystyrene tubes manufactured by LKB (Sweden) have been used as solid-phase carriers. The results evidence the possibility of applying EIA to the determination of HLA antigens.

[Sorption properties of polystyrene plates used in immunoenzyme analyses]. / Izuchenie sorbtsionnykh svoistv polistirolovykh planshetov, ispol'zuemykh v immunofermentnom analize.

The capacity of polystyrene carriers used in the enzyme immunoassay (EIA) for adsorbing 131I-labeled human serum albumin under different conditions has been studied, and the comparison of the plates manufactured by Dynatech AG (Switzerland) and by the Leningrad Works of Medical Polymers has been made. At the first stages of the reaction the antigen is separated from the carrier and the amount of the desorbed antigen depends on its initial dose and the dilution of the assayed sera. The irregular desorption of the antigen leads to misinterpretation of the results. Comparison of the polystyrene plates has shown that each plate is characterized by individual adsorption capacity, which impedes at present the standardization of EIA-based test systems.

[An immunoenzyme method of determining benzylpenicillin in biological substrates and its antibodies in immunized animals]. / Immunofermentnyi metod dlia opredeleniia benzilpenitsillina v biologicheskikh substratakh i antitel k nemu u immunizirovannykh zhivotnykh.

Lately it appeared to be necessary to develop-alternative methods for control of antibiotic therapy. Detection of low molecular substances based on the immunoenzymatic assay (IEA) is one of the most promising approaches to the development of such methods. The authors developed a quantitative method for determination of benzylpenicillin (BP) according to the procedure described earlier by Van Weemen. The BP antisera were prepared by immunization of rabbits with a conjugate of bovine serum albumin. The gamma-globulin fraction isolated from the antisera was conjugated with peroxidase. The working dilution of the conjugate was determined by the chess titration method. The test system of competitive binding developed by the authors provides detection of BP in the biological fluids in concentrations of 0.1 to 0.0001 microgram/ml (by BP dry weight).