[Prediction of a non-small cell lung cancer sensitivity to cisplatin and paclitaxel based on the marker genes expression].

The goal of the present study was to define gene expression signatures that predict a chemosensitivity of non-small cell lung cancer (NSCLC) to cisplatin and paclitaxel. To generate set of candidate genes likely to be predictive a current knowledge of the pathways involved in resistance and sensitivity to individual drugs was used. Forty four genes coding proteins belonging to following categories: ATP-dependent transport proteins, detoxification system proteins, reparation system proteins, tubulin and proteins responsible for its synthesis, cell cycle and apoptosis proteins were considered. Eight NSCLC cell lines (A549, Calul, H1299, H322, H358, H460, H292, and H23) were used in our study. For each NSCLC cell line a cisplatin and paclitaxel chemosensitivity as well as an expression level of 44 candidate genes were evaluated. To develop a chemosensitivity prediction model based on selected genes expression level a multiple regression analysis was performed. The model based on the expression level of 11 genes (TUBB3, TXR1, MRP5, MSH2, ERCC1, STMN, SMAC, FOLR1, PTPN14, HSPA2, GSTP1) allowed us to predict the paclitaxel cytotoxic concentration with high level of correlation (r = 0.91, p < 0.01). However, none model developed was able to reliably predict a sensitivity of the NSCLC cells to cisplatin.

[Electron microscopic analysis of the structure of metaphase chromosomes after liposome treatment]. / Elektronno-mikroskopicheskii analiz struktury metafaznykh khromosom posle obrabotki ikh liposomami.

The fine structure of the methaphase chromosomes from the Chinese hamster cell culture was studied after the incubation with lyposomes isolated from hen egg yolk phosphatidilcholine, or from the total rat liver phospholipid. It has been found that during incubation lyposomes surround chromosomes, form a multifold covering considerably decondensing the chromosome matrix. The data obtained indicate that the analogues of cell membrane - lyposomes - may be involved in the regulation of the structural organization of metaphase chromosomes.