Activity of isoflavone in managing polycystic ovary syndrome symptoms (Review).

Polycystic ovary syndrome (PCOS), a hormonal and metabolic disorder manifested in women of reproductive age, is still being treated using drugs with side effects. As an alternative to these drugs, isoflavone, also identified as phytoestrogen, has anti-PCOS activity. Isoflavone can help relieve PCOS symptoms by lowering the level of testosterone, which causes hyperandrogenism, thereby normalizing the menstrual cycle and restoring normal ovarian morphology. Furthermore, isoflavone influences the improvement of the metabolic profile, which changes because of PCOS, as well as the reduction of inflammatory markers and oxidative stress. However, both significant and non-significant results have been generated on the activity of isoflavones in PCOS. The present review aims to discuss the existing literature on the effect of isoflavone on PCOS symptoms based on in vivo and clinical trial studies.

Diving into paclitaxel: isolation and screening content from Taxus sumatrana at Singgalang Conservation Center, West Sumatra.

The report features the first isolation of paclitaxel from wood of conservated Taxus sumatrana. The T. sumatrana is a nationally protected endemic plant that has been successfully cultivated outside its natural habitat at the Singgalang Conservation Centre in West Sumatra. The paclitaxel was utilised as a reference standard for evaluating its presence in different parts of T. sumatrana. This analysis exhibits that the acetone extract from T. sumatrana bark contained the highest paclitaxel concentration, measuring about 0.473 ± 0.031 ppm. The isolated paclitaxel demonstrated potent cytotoxic activity against A549, HeLa, and MCF7 cancer cells, by IC50 values of 3.26 ± 0.334, 2.85 ± 0.257, and 3.81 ± 0.013 µM, respectively. This outcome provides scientific support for conservation programs and campaigns for the community to engage in conservation efforts.

Inhibitory activity of xanthoangelol isolated from Ashitaba (Angelica keiskei Koidzumi) towards α-glucosidase and dipeptidyl peptidase-IV: in silico and in vitro studies.

In Indonesia, the sap of Angelica keiskei Koidzumi has been utilized traditionally as a blood-sugar reducer, nonetheless, its molecular mechanism still needs to be studied. This study aimed to isolate xanthoangelol (XA) from the yellow sap of A. keiskei planted in Mount Rinjani, Indonesia, and to investigate its mechanism by in silico and in vitro methods towards α-glucosidase and dipeptidyl peptidase-IV (DPP-IV). The dried yellow sap was macerated using ethanol, subjected to liquid-liquid extraction using a different polarity of solvents, further gradient-eluted with column chromatography. The isolated compound, formed as yellow crystals, melting point 114-114.4 °C, λmax 368 nm, m/z 393.20 [M + H]+, was confirmed as XA. Acarbose, an α-glucosidase inhibitor, and sitagliptin, a DPP-IV inhibitor, respectively, were employed as the reference drugs for both the in silico and in vitro studies. XA interacts with essential amino acid residues 232-237 in the N-terminal N-loop of α-glucosidase by forming a hydrogen bond with Ala234, a salt-bridge with Asp232, and 9 hydrophobic interactions (binding energy -7.81 kcal/mol; Ki = 1.99 µM). These binding modes resemble those of acarbose. Moreover, XA forms hydrogen bonds with Glu205 and Glu206 in the subsite S2 and π-π interaction with Phe357 in the extensive subsite S2 of DPP-IV (binding energy -8.34 kcal/mol; Ki = 0.873 µM), which are similar to those of sitagliptin. XA inhibits both α-glucosidase (IC50 XA = 14.45 µM; IC50 acarbose = 207 µM) and DPP-IV (IC50 XA = 10.49 µM; IC50 sitagliptin = 0.87 µM). Taken together, XA isolated from the yellow sap of A. keiskei Koidzumi might possess the potential to be further developed as an inhibitor of α-glucosidase and DPP-IV.

Protective Effect of Quercetin Administration in Bacterialinduced Periodontitis on Rats

@#Introduction: Quercetin is a flavonoid found in a variety of plants, including guava, apples, onions, and tea. It’s been used as an anti-oxidant and anti-inflammatory substance for a long time. This study aims to investigate the effect of quercetin on periodontitis caused by Porphyromonas gingivalis-adhered ligatures. Methods: Eighteen male adult Sprague Dawley rats were divided into 3 groups, namely the control group (C, n=6) and the other two groups that received quercetin at 45mg/kg/day as a preventive (Qp, n=6) and a curative treatment (Qc, n=6), respectively. Under general anaesthesia, periodontitis was induced by placing a 3/0 non-resorbable sterile silk thread around the mandibular incisor teeth of eighteen male adult Sprague Dawley rats. The ligature placement caused severe irritation in the periodontal tissue. The animals were euthanized after 14 days of post-induction treatment, and samples of the mandibular portion were kept in formalin and prepared for histological processing to determine the grade of inflammation (GI). The periodontal pocket depth (PPD) was measured using the Michigan-O probe with Williams marks at the mesial and lingual sites of the rat’s incisors tooth to determine the clinical parameter. Results: Qp showed the best improvement, in both parameters, clinically (PPD score, p=0,0018 at the lingual site, and p=0,0264 at the mesial site) and histologically (GI, p=0,0002). Significant differences were found in preventing clinical attachment-loss statistically (p<0,05) on Qp, better than the Qc at an equal dose (p<0,05). Conclusion: This finding suggests that quercetin administered as a preventive measure (Qp) may promote the healing process of gingiva in periodontitis conditions better than the control group and curative group (Qc).