Knowledge and Practice of the Referral System under the National Health Insurance Scheme among Medical and Dental Practitioners of A Large-Accredited Facility in Kano, Nigeria.

BACKGROUND: Studies on healthcare professionals' knowledge about the National Health Insurance Scheme (NHIS) are scarce. Therefore, we assessed the knowledge and practice of the NHIS referral system among Medical and Dental practitioners in a tertiary hospital in Northwest Nigeria. METHODS: This cross-sectional study involved 242 medical and dental practitioners randomly selected from nine departments for over 6-weeks. A structured self-administered questionnaire was used to collect data. Data were analyzed using descriptive and inferential statistics. RESULTS: The respondents' mean age was 35.7±6.0 years; they were predominantly males (64.9%). Their mean overall knowledge score was 58.9±23.0%, with 66.9% of respondents having inadequate overall knowledge of the NHIS referral system. Practice department (Fishers 2 exact, P=0.0019), perceived knowledge of the referral system (ꭓ =8.169, P=0.004), and having been referred as an enrolee (ꭓ2 = 6.358, P=0.012) were associated with overall-knowledge. Obstetrics-and-Gynaecology (odds ratio[OR]=0.29, 95% confident interval [CI] [0.88-0.98]), Dental and-Maxillofacial-Surgery (OR=0.08, 95%CI[0.01-0.98]), and Otorhinolaryngology (OR=0.18, 95%CI[0.04-0.80]) respondents were less likely to have adequate overall-knowledge.Although 56.2%, 50.4%, 20.7%, and 89.7% were enrolees, had received treatment as enrolees, had been referred as enrolees and treated other enrolees, respectively, an unimpressive proportion had sighted a referral letter (64.9%) or authorization code on the letter (25.2%), referred an enrolee from their department previously (51.2%) or used the NHIS referral form to write referrals (38.8%). CONCLUSION: The overall knowledge of the NHIS referral system was inadequate. The practice of the referral system was below expectation. Therefore, training medical and dental practitioners on the NHIS referral system is necessary. Training should target those who are least likely to have adequate overall knowledge.

CONTEXTE: Les études sur les connaissances des professionnels de la santé concernant le Régime d'assurance maladie national (NHIS) sont rares. Nous avons donc évalué les connaissances et la pratique du système de référence du NHIS parmi les médecins et dentistes d'un hôpital tertiaire du Nord-Ouest du Nigeria. MÉTHODES: Cette étude transversale a impliqué 242 médecins et dentistes sélectionnés de manière aléatoire dans neuf départements pendant plus de 6 semaines. Un questionnaire structuré auto-administré a été utilisé pour recueillir des données. Les données ont été analysées à l'aide de statistiques descriptives et inférentielles. RÉSULTATS: L'âge moyen des répondants était de 35,7 ± 6,0 ans ; ils étaient principalement des hommes (64,9 %). Leur score moyen global de connaissances était de 58,9 ± 23,0 %, avec 66,9 % des répondants ayant une connaissance globale insuffisante du système de référence du NHIS. Le département de pratique (test exact de Fisher, P=0,0019), la connaissance perçue du système de référence (ꭓ 2 =8,169, P=0,004) et avoir été référé en tant qu'adhérent (ꭓ 2 = 6,358, P=0,012) étaient associés à la connaissance globale. Les répondants en obstétrique-gynécologie (rapport des cotes [OR]=0,29, intervalle de confiance à 95 % [IC] [0,88-0,98]), en chirurgie dentaire et maxillo-faciale (OR=0,08, IC à 95 % [0,01-0,98]), et en oto-rhino-laryngologie (OR=0,18, IC à 95 % [0,04-0,80]) étaient moins susceptibles d'avoir une connaissance globale adéquate. Bien que 56,2 %, 50,4 %, 20,7 % et 89,7 % étaient adhérents, avaient reçu un traitement en tant qu'adhérents, avaient été référés en tant qu'adhérents et avaient traité d'autres adhérents, respectivement, une proportion peu impressionnante avait vu une lettre de référence (64,9 %) ou un code d'autorisation sur la lettre (25,2 %), avait référé un adhérent de leur département précédemment (51,2 %) ou avait utilisé le formulaire de référence du NHIS pour écrire des références (38,8 %). CONCLUSION: La connaissance globale du système de référence du NHIS était insuffisante. La pratique du système de référence était en deçà des attentes. Par conséquent, la formation des médecins et dentistes sur le système de référence du NHIS est nécessaire. La formation devrait cibler ceux qui sont moins susceptibles d'avoir une connaissance globale adéquate. MOTS-CLÉS: Connaissances et Pratiques, Professionnels de la santé, Régime national d'assurance maladie, Nigeria, Renvoi.

Restoration of the ER stress response protein TDAG51 in hepatocytes mitigates NAFLD in mice.

Endoplasmic reticulum stress is associated with insulin resistance and the development of nonalcoholic fatty liver disease. Deficiency of the endoplasmic reticulum stress response T-cell death-associated gene 51 (TDAG51) (TDAG51-/-) in mice promotes the development of high-fat diet (HFD)-induced obesity, fatty liver, and hepatic insulin resistance. However, whether this effect is due specifically to hepatic TDAG51 deficiency is unknown. Here, we report that hepatic TDAG51 protein levels are consistently reduced in multiple mouse models of liver steatosis and injury as well as in liver biopsies from patients with liver disease compared to normal controls. Delivery of a liver-specific adeno-associated virus (AAV) increased hepatic expression of a TDAG51-GFP fusion protein in WT, TDAG51-/-, and leptin-deficient (ob/ob) mice. Restoration of hepatic TDAG51 protein was sufficient to increase insulin sensitivity while reducing body weight and fatty liver in HFD fed TDAG51-/- mice and in ob/ob mice. TDAG51-/- mice expressing ectopic TDAG51 display improved Akt (Ser473) phosphorylation, post-insulin stimulation. HFD-fed TDAG51-/- mice treated with AAV-TDAG51-GFP displayed reduced lipogenic gene expression, increased beta-oxidation and lowered hepatic and serum triglycerides, findings consistent with reduced liver weight. Further, AAV-TDAG51-GFP-treated TDAG51-/- mice exhibited reduced hepatic precursor and cleaved sterol regulatory-element binding proteins (SREBP-1 and SREBP-2). In vitro studies confirmed the lipid-lowering effect of TDAG51 overexpression in oleic acid-treated Huh7 cells. These studies suggest that maintaining hepatic TDAG51 protein levels represents a viable therapeutic approach for the treatment of obesity and insulin resistance associated with nonalcoholic fatty liver disease.

Characterization, antioxidant activity and potential application fractionalized Szechuan pepper on fresh beef meat as natural preservative.

The pericarp of Szechuan pepper is rich in phenols and alkylamides, making it a potential source of antioxidant compounds. Despite being recognized as the primary antioxidants in Szechuan pepper, there is still limited knowledge about their application in real food systems. This study aims to identify, separate, and apply polyphenol and alkylamide fractions derived from Szechuan extracts to beef meat. Using HPLC-MS2, we identified 5 phenols and 11 alkylamides in Szechuan extracts. The quality of the minced meat was evaluated based on color, thiobarbituric acid reactive substances (TBARS), conjugated dienes, carbonyl content, Sulfhydryl content, microbiological content, and total volatile basic nitrogen content (TVB-N). Compared to the polyphenol fraction (1.25 mg/mL), alkylamide fraction (25 mg/mL), and control samples, beef samples incorporated with the polyphenol fraction (6.25 mg/mL) significantly reduced carbonyl content, TBARS, and TVB-N values at the end of storage. Furthermore, they exhibited a significant slowdown in microbial development, improved meat color stability, and preserved pH. Therefore, the use of Szechuan pepper fractions as natural preservatives in meat and meat products is an important area of research and has the potential to enhance the safety and quality of meat products.

DIETARY LIFESTYLE, HYPOALBUMINAEMIA AND ANAEMIA AMONG OLDER ADULTS ATTENDING A PRIMARY CARE CLINIC IN NIGERIA: A CROSS-SECTIONAL STUDY.

Introduction: Anaemia has been extensively studied in children and women of reproductive age. However, there are few data on anaemia and related conditions in the elderly from developing countries. Objectives: To determine the prevalence and severity pattern of anaemia, and assess the relationship between dietary lifestyle, hypoalbuminaemia, and anaemia in older persons. Methodology: A cross-sectional study involving 378 patients aged≥60 years who presented at the General Outpatient Clinic. Dietary lifestyle was assessed using a structured questionnaire. Samples were collected for packed cell volume and serum albumin. Inferential statistical analyses were used to determine the associations between variables. Results: A total of 348 respondents completed the study. The mean age of respondents was 67.83 ±7.53 years with female (60.9%) predominance. The prevalence of anaemia and hypoalbuminaemia were 42.2% and 17.8% respectively. Hypoalbuminaemia (ß=0.335, 95%CI=0.131-0.229, P<0.001), long duration of co-morbidities (ß= - 0.179, 95%CI= -0.165-0.047, P<0.001), one full meal/day (ß=0.130, 95%CI=0.224-1.879, P=0.013), and low monthly income (ß=0.122, 95%CI=0.179-1.543, P=0.026) were the predictors of anaemia among the elderly in this study. Conclusion: Data on the predictors of anaemia from this study will be useful in developing guidelines and strategies for managing the condition in primary care settings and other similar sites.

Predictive and Relative factors of Dental Anxiety among Third Molar Disimpaction Patients in a Northern Nigerian Hospital.

INTRODUCTION: Various factors influence the expression of dental anxiety (DA) among patients. Knowledge of these factors may aid the management of mandibular third molar (M3) dis-impaction patients. AIM: The study aimed to determine the predictive and relative factors of DA among Mandibular third molar (M3) dis-impaction patients at the Aminu Kano Teaching Hospital (AKTH), Kano, Nigeria. METHODOLOGY: The study was a cross-sectional type conducted at the Oral Surgery clinic of AKTH, Kano among M3 dis-impaction patients between September 2016 and August 2017. Biodata and information on the past dental history of the participants were collected and recorded. The level of anxiety of participants was assessed with the Modified Dental Anxiety Scale (MDAS). Data were analyzed using Statistical Package for Social Sciences (SPSS) for windows (IBM SPSS statistic version 23). The significance level in the study was set at p ≤ 0.05. RESULTS: One hundred and sixteen patients comprising 62(55.2%) males and 54(44.8%) females were recruited. The sample's median age was 27 years and the majority of the participants were students (n=49,42.2%). The participants' MDAS scores ranged from 5 to 22 with a median score of 11. Females (median MDAS score of 12.50) were significantly (p<0.0001) more anxious than males (median MDAS score of 9.00). Bivariate analysis showed statistically significant differences in DA of the categories of the following independent variables - gender (p<0.0001), ethnicity (p=0.041), occupational status (p=0.044), previous surgical extraction experience (p=0.006), previous bad dental treatment experience (p<0.0001) and history of postponement of dental treatment due to anxiety(p<0.0001). Binary logistic regression analysis with these variables as independent predictors of DA showed that only gender (p=0.013) and previous surgical extraction experience (p=0.042) had statistically significant odds of predicting dental anxiety among the patients. Females had significantly higher odds of being anxious than males [adjusted odds ratio (OR) = 4.180, 95% Confidence Interval (CI)=1.359 to 12.852] and patients with previous surgical extraction experience had higher odds of being anxious than those without surgical extraction experience [adjusted OR=3.65, 95% CI=1.02 to 13.03]. CONCLUSION: The predictors of DA in the study are female gender and previous surgical extraction experience. These and other DA-related factors such as patients' occupation and previous bad dental treatment experiences should be considered preoperatively to identify and manage anxious M3 dis-impaction patients.

INTRODUCTION: Divers facteurs influencent l'expression de l'anxiété dentaire (AD) chez les patients. La connaissance de ces facteurs peut aider à la prise en charge des patients subissant l'extraction des troisièmes molaires mandibulaires (M3). OBJECTIF: L'étude visait à déterminer les facteurs prédictifs et relatifs de l'AD chez les patients subissant l'extraction des troisièmes molaires mandibulaires (M3) à l'Hôpital Universitaire Aminu Kano (AKTH) de Kano, au Nigeria. MÉTHODOLOGIE: L'étude était de type transversal et a été menée à la clinique de chirurgie buccale de l'AKTH, à Kano, auprès de patients subissant l'extraction des M3 entre septembre 2016 et août 2017. Les données biométriques et les antécédents dentaires des participants ont été recueillis et enregistrés. Le niveau d'anxiété des participants a été évalué à l'aide de l'Échelle d'Anxiété Dentaire Modifiée (MDAS). Les données ont été analysées à l'aide du logiciel Statistical Package for Social Sciences (SPSS) pour Windows (version IBM SPSS 23). Le seuil de signification de l'étude a été fixé à p ≤ 0,05. RÉSULTATS: Cent seize patients, dont 62 (55,2 %) hommes et 54 (44,8 %) femmes, ont été recrutés. L'âge médian de l'échantillon était de 27 ans et la majorité des participants étaient des étudiants (n=49, 42,2 %). Les scores MDAS des participants variaient de 5 à 22, avec un score médian de 11. Les femmes (score MDAS médian de 12,50) étaient significativement (p<0,0001) plus anxieuses que les hommes (score MDAS médian de 9,00). L'analyse bivariée a montré des différences statistiquement significatives dans l'AD des catégories des variables indépendantes suivantes : le sexe (p<0,0001), l'ethnicité (p=0,041), le statut professionnel (p=0,044), l'expérience d'extraction chirurgicale antérieure (p=0,006), l'expérience antérieure de mauvais traitement dentaire (p<0,0001) et l'histoire du report du traitement dentaire en raison de l'anxiété (p<0,0001). L'analyse de régression logistique binaire avec ces variables comme prédicteurs indépendants de l'AD a montré que seul le sexe (p=0,013) et l'expérience d'extraction chirurgicale antérieure (p=0,042) avaient des cotes de prédiction statistiquement significatives de l'anxiété dentaire chez les patients. Les femmes avaient des chances significativement plus élevées d'être anxieuses que les hommes [rapport de cotes ajusté (OR) = 4,180, intervalle de confiance à 95 % (IC) = 1,359 à 12,852] et les patients ayant déjà subi une extraction chirurgicale avaient des chances plus élevées d'être anxieux que ceux n'ayant pas d'expérience d'extraction chirurgicale [OR ajusté = 3,65, IC à 95 % = 1,02 à 13,03]. CONCLUSION: Les facteurs prédictifs de l'AD dans l'étude sont le sexe féminin et l'expérience antérieure d'extraction chirurgicale. Ces facteurs liés à l'AD et d'autres facteurs tels que l'occupation des patients et les expériences antérieures de mauvais traitement dentaire devraient être pris en compte en préopératoire pour identifier et prendre en charge les patients anxieux subissant l'extraction des M3. Mots-clés: anxiété dentaire, facteurs, troisième molaire, extraction.

Serological Profiles of SARS-Cov-2 among Adult Outpatients and Caregivers of a Family Medicine Clinic in Northern Nigeria.

BACKGROUND: Serological evaluation is essential for investigating the extent of COVID-19 in the studied population and evaluating the potential effectiveness of serum antibodies as a protective factor against future disease. OBJECTIVE: The study aimed to provide data on the seroprevalence and pattern of immune response to SARS-CoV-2 infection and the associated factors among outpatients and their caregivers. METHODS: A cross-sectional study involving 208 patients and caregivers who presented at the Family Medicine Clinic. A structured self-administered questionnaire was used to collect data on sociodemographic and COVID-19-related factors. The SARS-CoV-2 antibodies were assayed with the Realy Tech Rapid Test Device. Inferential statistical analyses were used to determine the associations between SARS-CoV-2 antibody outcomes and other variables. RESULTS: The ages of the participants ranged from 18 to 80 years, with a mean age of 38.87± 11.82 years and with female preponderance. The crude prevalence of anti-SARS-CoV-2 seropositivity was 20.7% (95% CI=1.14-1.28); of which 17.1% was for IgG, 2.9% for IgM, and 0.7% for both IgG and IgM. The overall corrected prevalence was 20.3%. The only identified predictor of anti-SARS-CoV-2 seropositivity in this study was the previous family history of COVID-19 infection (OR=36.548, CI=1.371-74.364, p=0.032). CONCLUSION: The identified predictor and other important outcomes will be useful in forming strategies for the prevention and management of COVID-19 infection.

CONTEXTE: L'évaluation sérologique est essentielle pour déterminer l'étendue du COVID-19 dans la population étudiée et évaluer l'efficacité potentielle des anticorps sériques en tant que facteur de protection contre une maladie future. OBJECTIF: L'étude visait à fournir des données sur la séroprévalence et le profil de la réponse immunitaire à l'infection par le SRAS-CoV-2 et les facteurs associés chez les patients ambulatoires et leurs soignants. MÉTHODES: Il s'agit d'une étude transversale portant sur 208 patients et soignants qui se sont présentés à la clinique de médecine familiale. Un questionnaire structuré auto-administré a été utilisé pour recueillir des données sur les facteurs sociodémographiques et liés au COVID-19. Les anticorps antiSRAS-CoV-2 ont été dosés à l'aide du dispositif de test rapide Realy Tech. Des analyses statistiques inférentielles ont été utilisées pour déterminer les associations entre les résultats des anticorps anti-SRAS-CoV-2 et d'autres variables. RÉSULTATS: Les participants étaient âgés de 18 à 80 ans, avec un âge moyen de 38,87± 11,82 ans et une prépondérance féminine. La prévalence brute de la séropositivité anti-SARSCoV-2 était de 20,7 % (IC à 95 % = 1,14-1,28), dont 17,1 % pour les IgG, 2,9 % pour les IgM et 0,7 % pour les IgG et les IgM. La prévalence globale corrigée était de 20,3 %. Le seul facteur prédictif identifié de la séropositivité anti-SARS-CoV-2 dans cette étude était les antécédents familiaux d'infection par COVID-19 (OR=36,548, CI=1,371-74,364, p=0,032). CONCLUSION: Le facteur prédictif identifié et d'autres résultats importants seront utiles pour élaborer des stratégies de prévention et de prise en charge de l'infection par COVID-19. Mots-clés: Profil sérologique, SRAS-CoV-2, patients externes, soignants et nord du Nigeria.

Recent advances in rare earth ion-doped upconversion nanomaterials: From design to their applications in food safety analysis.

The misuse of chemicals in agricultural systems and food production leads to an increase in contaminants in food, which ultimately has adverse effects on human health. This situation has prompted a demand for sophisticated detection technologies with rapid and sensitive features, as concerns over food safety and quality have grown around the globe. The rare earth ion-doped upconversion nanoparticle (UCNP)-based sensor has emerged as an innovative and promising approach for detecting and analyzing food contaminants due to its superior photophysical properties, including low autofluorescence background, deep penetration of light, low toxicity, and minimal photodamage to the biological samples. The aim of this review was to discuss an outline of the applications of UCNPs to detect contaminants in food matrices, with particular attention on the determination of heavy metals, pesticides, pathogenic bacteria, mycotoxins, and antibiotics. The review briefly discusses the mechanism of upconversion (UC) luminescence, the synthesis, modification, functionality of UCNPs, as well as the detection principles for the design of UC biosensors. Furthermore, because current UCNP research on food safety detection is still at an early stage, this review identifies several bottlenecks that must be overcome in UCNPs and discusses the future prospects for its application in the field of food analysis.

Au-Ag OHCs-based SERS sensor coupled with deep learning CNN algorithm to quantify thiram and pymetrozine in tea.

Pesticide residue detection in food has become increasingly important. Herein, surface-enhanced Raman scattering (SERS) coupled with an intelligent algorithm was developed for the rapid and sensitive detection of pesticide residues in tea. By employing octahedral Cu2O templates, Au-Ag octahedral hollow cages (Au-Ag OHCs) were developed, which improved the surface plasma effect via rough edges and hollow inner structure, amplifying the Raman signals of pesticide molecules. Afterward, convolutional neural network (CNN), partial least squares (PLS), and extreme learning machine (ELM) algorithms were applied for the quantitative prediction of thiram and pymetrozine. CNN algorithms performed optimally for thiram and pymetrozine, with correlation values of 0.995 and 0.977 and detection limits (LOD) of 0.286 and 29 ppb, respectively. Accordingly, no significant difference (P greater than 0.05) was observed between the developed approach and HPLC in detecting tea samples. Hence, the proposed Au-Ag OHCs-based SERS technique could be utilized for quantifying thiram and pymetrozine in tea.

Socio-economic and Lifestyle Determinants of Functional Capacity of the Senior Attendees of an Outpatient Clinic in Northern Nigeria: A Cross-Sectional Study.

BACKGROUND: Typical ageing changes and the associated health concerns frequently manifest themselves as reduction in their efficiency and functional capacity. OBJECTIVE: To identify the socioeconomic and lifestyle determinants of the functional capacity of elderly patients. METHODS: A cross-sectional study involving 329 patients aged ≥ 60 years who presented to the General Outpatient Clinic. Data on socioeconomic, lifestyle, and functional capacity were collected. The functional capacity was assessed by the self-reported questionnaires: the Lawton and Katz indexes for the activity of daily living (ADL) and instrumental activity of daily living (IADL) respectively. The chi-square test and logistic regression analysis were used to determine associations between the variables. The level of significance was set at a p-value ≤ 0.05. RESULTS: A total of 312 respondents completed the study, with 59.6% females and a mean age of 67.67 ± 7.69 years. The majority of the respondents (76.3%) belong to the low socioeconomic status (classes V and VI). The prevalence of functional dependence was 21.5% and 44.2% for ADL and IADL respectively. The disability in continence and food preparation had the highest prevalence among the components of ADL and IADL respectively. Advancing age, Hausa/Fulani tribe, polygamy, poor social support, and chronic pain were the determinants of functional dependence in ADL while age, female gender, marital status, and Fulani tribe were the determinants of functional dependence in IADL among the respondents.CONCLUSION: The identified determinants of functional capacity among the older persons should be considered when assessing their functional capacity in the primary care or similar settings.

CONTEXTE: Les changements typiques liés au vieillissement et les problèmes de santé qui y sont associés se manifestent souvent par une réduction de l'efficacité et de la capacité fonctionnelle. OBJECTIFS: Identifier les déterminants socio-économiques et le mode de vie de la capacité fonctionnelle des patients âgés. MÉTHODES: Étude transversale portant sur 329 patients âgés de ≥ 60 ans qui se sont présentés à la consultation externe générale. Des données sur la situation socio-économique, le mode de vie et la capacité fonctionnelle ont été recueillies. La capacité fonctionnelle a été évaluée à l'aide de questionnaires autodéclarés : les indices de Lawton et de Katz pour l'activité de la vie quotidienne (ADL) et l'activité instrumentale de la vie quotidienne (IADL) respectivement. Le test du chi carré et l'analyse de régression logistique ont été utilisés pour déterminer les associations entre les variables. Le niveau de signification a été fixé à une valeur p ≤ 0,05. RÉSULTATS: Au total, 312 personnes ont répondu à l'étude, dont 59,6 % de femmes et un âge moyen de 67,67 ±7,69 ans. La majorité des personnes interrogées (76,3 %) appartiennent à un statut socioéconomique faible (classes V et VI). La prévalence de la dépendance fonctionnelle était de 21,5 % et 44,2 % pour les AVQ et les AIVQ respectivement. Les incapacités liées à la continence et à la préparation des repas ont la prévalence la plus élevée parmi les composantes des AVQ et des AIVQ respectivement. L'âge avancé, la tribu Hausa/Fulani, la polygamie, le manque de soutien social et la douleur chronique étaient les déterminants de la dépendance fonctionnelle dans les AVQ, tandis que l'âge, le sexe féminin, l'état matrimonial et la tribu Fulani étaient les déterminants de la dépendance fonctionnelle dans les AIVQ parmi les personnes interrogées. CONCLUSION: Les déterminants identifiés de la capacité fonctionnelle chez les personnes âgées devraient être pris en compte lors de l'évaluation de leur capacité fonctionnelle dans le cadre des soins primaires ou dans des contextes similaires. Mots clés: Capacité fonctionnelle, Socio-économie, Mode de vie, Personnes âgées et nord du Nigeria.

Ultrasensitive fluorescence sensor for Hg2+ in food based on three-dimensional upconversion nanoclusters and aptamer-modulated thymine-Hg2+-thymine strategy.

Mercury (Hg2+) is a potentially toxic heavy metal ion found to be drastically deleterious to humans. Herein, an ultrasensitive fluorescence sensor was developed using three-dimensional upconversion nanoclusters (EBSUCNPs) and aptamer-modulated thymine-Hg2+-thymine strategy. The EBSUCNPs were used as the energy donors, the PDANPs served as the acceptors, and the aptamer was applied as an identification tag for Hg2+. Due to the energy transfer effect, the fluorescence of EBSUCNPs can be effectively quenched by Polydopamine nanoparticles (PDANPs). In the existence of Hg2+, T (thymine)-rich aptamers between EBSUCNPs and PDANPs were hybridized with Hg2+ to yield thymine-Hg2+-thymine and folded back to hairpin structure, causing PDANPs to detach from the EBSUCNPS and the recovery of fluorescence. Under optimum conditions, the linear sensing range of Hg2+ was 0.5-20 µg/L, and the detection limit was 0.28 µg/L. Furthermore, it exhibited excellent selectivity and anti-interference, which made it an ideal method for identifying Hg2+ in spiked samples.

Fusion-based strategy of CSA and mobile NIR for the quantification of free fatty acid in wheat varieties coupled with chemometrics.

The use of sensor fusion, a novel method of combining artificial senses, has become increasingly popular in the assessment of food quality. This study employed a combination of the colorimetric sensor array (CSA) and mobile near-infrared (NIR) spectroscopy to predict free fatty acids in wheat flour. In conjunction with a partial least squares model, Low- and mid-level fusion strategies were used for quantification. Accordingly, performance of the built model was evaluated based on higher correlation coefficients between calibration and prediction (RC and RP), lower root mean square error of prediction (RMSEP), and a higher residual predictive deviation (RPD). The mid-level fusion coupled PLS model produced superior data fusion findings, with RC = 0.8793, RMSECV = 7.91 mg/100 g, RP = 0.8747, RMSEP = 6.99 mg/100 g, and RPD = 2.27. The findings of the study suggest that the NIR-CSA fusion approach could be effectively applied to the prediction of free fatty acids in wheat flour.

Stability of starch-proanthocyanidin complexes to in-vitro amylase digestion after hydrothermal processing.

Proanthocyanidins (PA) form poorly digestible complexes with starch. The study examined amylase degradation mechanism and hydrothermal stability of starch-PA complexes. Sorghum-derived PA was complexed with wheat starch, reconstituted into flour (10% gluten added) and processed into crackers and pancakes. In vitro digestion profile of the complexes and products were characterized. The starch-PA complexes retained more (34-84%) fragments with degree of polymerization (DP) > 6,000 after 120 min digestion than controls (0-21%). Debranching further revealed higher retention of DP 11 - 30 chains in the digested starch-PA complexes than controls, suggesting amylopectin complexation contributed to reduced starch digestion. Starch-PA complexes retained reduced digestibility (50-56% higher resistant starch vs controls) in the cracker, but not pancake model. However, removing gluten from the pancake formulation restored the reduced digestibility of the starch-PA complexes. The starch-PA complexes are stable to hydrothermal processing, but can be disrupted by hydrophobic gluten proteins under excess moisture conditions.

Flexible SERS sensor using AuNTs-assembled PDMS film coupled chemometric algorithms for rapid detection of chloramphenicol in food.

The misuse of chloramphenicol (CAP) has led to the development of drug-resistant strains that pose significant threats to public health. Here, we propose a universal flexible surface-enhanced Raman spectroscopy (SERS) sensor utilizing gold nanotriangles (AuNTs) and polydimethylsiloxane (PDMS) film for rapid detection of CAP in food samples. Initially, AuNTs@PDMS with unique optical and plasmonic properties were used to collect spectra of CAP. Afterward, four chemometric algorithms were executed and compared. Accordingly, random frog-partial least squares (RF-PLS) exhibited optimum results with correlation coefficient of prediction (Rp = 0.9802) and the lowest root-mean-square error of prediction (RMSEP = 0.348 µg/mL). Furthermore, the sensor's efficacy to detect CAP in milk samples was confirmed, and the findings were compatible with the conventional HPLC approach (P > 0.05). Therefore, the proposed flexible SERS sensor could effectively be used to monitor milk quality and safety.

A Novel Colorimetric Sensor Array Coupled Multivariate Calibration Analysis for Predicting Freshness in Chicken Meat: A Comparison of Linear and Nonlinear Regression Algorithms.

As a source of vital nutrients for the normal functioning of the body, chicken meat plays an important role in promoting good health. This study examines the occurrence of total volatile basic nitrogen (TVB-N) as an index for evaluating freshness, using novel colorimetric sensor arrays (CSA) in combination with linear and nonlinear regression models. Herein, the TVB-N was determined by steam distillation, and the CSA was fabricated via the use of nine chemically responsive dyes. The corresponding dyes utilized, and the emitted volatile organic compounds (VOCs) were found to be correlated. Afterwards, the regression algorithms were applied, assessed, and compared, with the result that a nonlinear model based on competitive adaptive reweighted sampling coupled with support vector machines (CARS-SVM) achieved the best results. Accordingly, the CARS-SVM model provided improved coefficient values (Rc = 0.98 and Rp = 0.92) based on the figures of merit used, as well as root mean square errors (RMSEC = 3.12 and RMSEP = 6.75) and a ratio of performance deviation (RPD) of 2.25. Thus, this study demonstrated that the CSA paired with a nonlinear algorithm (CARS-SVM) could be employed for fast, noninvasive, and sensitive detection of TVB-N concentration in chicken meat as a major indicator of freshness in meat.

Stimuli-responsive SERS biosensor for ultrasensitive tetracycline sensing using EDTA-driven PEI@CaCO3 microcapsule and CS@FeMMs.

In this work, a stimuli-responsive SERS biosensor was fabricated for tetracycline (TTC) by "signal-on" strategy using (EDTA)-driven polyethyleneimine grafted calcium carbonate (PEI@CaCO3) microcapsule and chitosan-Fe magnetic microbeads (CS@FeMMs). Initially, aptamer conjugated magnetic-bead CS@FeMMs@Apt with superparamagnetism and excellent biocompatibility was employed as capture probe, which facilitated the rapid and easy magnetic separation. Subsequently, the PEI cross-linked layer and aptamer network layer were constructed onto the outer layer of CaCO3@4-ATP microcapsule to form sensing probes (PEI@CaCO3@4-ATP@Apt) via the layer-by-layer assembly method. In the presence of TTC, a sandwich SERS-assay was exploited by aptamer recognition induced target-bridged strategy. When the solution of EDTA was added, the core layer of CaCO3 would be dissolved quickly, destroying the microcapsule to release 4-ATP. The released 4-ATP could be quantitatively monitored by dripping the supernatant onto the AuNTs@PDMS SERS platform, resulting in a strong Raman "signal-on". Under the optimal conditions, a good linear relationship was established with a correlation coefficient (R2) of 0.9938 and a LOD of 0.03 ng/mL. Additionally, the application capacity of the biosensor to detect TTC was also affirmed in food matrixes, and the results were consistent with the standard ELISA method (P > 0.05). Hence, this SERS biosensor affords extensive application prospects for TTC detection with multiple merits such as high sensitivity, environment friendliness, and high stability.

Neuraminidase 4 (NEU4): new biological and physiological player.

Sialidases are found in viruses, bacteria, fungi, avians, and mammals. Mammalian sialidases differ in their specificity, optimum pH, subcellular localization, and tissue expression. To date, four genes encoding mammalian sialidases (NEU1-4) have been cloned. This review examines the functional impact of NEU4 sialidase on complex physiological and cellular processes. The intracellular localization and trafficking of NEU4 and its potential target molecules are discussed along with its impact on cancer, lysosomal storage disease, and cellular differentiation. Modulation of NEU4 expression may be essential not only for the breakdown of sialylated glycoconjugates, but also in the activation or inactivation of functionally important cellular events.

The effects of sugammadex vs. neostigmine on postoperative respiratory complications and advanced healthcare utilisation: a multicentre retrospective cohort study.

Reversing neuromuscular blockade with sugammadex can eliminate residual paralysis, which has been associated with postoperative respiratory complications. There are equivocal data on whether sugammadex reduces these when compared with neostigmine. We investigated the association of the choice of reversal drug with postoperative respiratory complications and advanced healthcare utilisation. We included adult patients who underwent surgery and received general anaesthesia with sugammadex or neostigmine reversal at two academic healthcare networks between January 2016 and June 2021. The primary outcome was postoperative respiratory complications, defined as post-extubation oxygen saturation < 90%, respiratory failure requiring non-invasive ventilation, or tracheal re-intubation within 7 days. Our main secondary outcome was advanced healthcare utilisation, a composite outcome including: 7-day unplanned intensive care unit admission; 30-day hospital readmission; or non-home discharge. In total, 5746 (6.9%) of 83,250 included patients experienced postoperative respiratory complications. This was not associated with the reversal drug (adjusted OR (95%CI) 1.01 (0.94-1.08); p = 0.76). After excluding patients admitted from skilled nursing facilities, 8372 (10.5%) patients required advanced healthcare utilisation, which was not associated with the choice of reversal (adjusted OR (95%CI) 0.95 (0.89-1.01); p = 0.11). Equivalence testing supported an equivalent effect size of sugammadex and neostigmine on both outcomes, and neostigmine was non-inferior to sugammadex with regard to postoperative respiratory complications or advanced healthcare utilisation. Finally, there was no association between the reversal drug and major adverse cardiovascular events (adjusted OR 1.07 (0.94-1.21); p = 0.32). Compared with neostigmine, reversal of neuromuscular blockade with sugammadex was not associated with a reduction in postoperative respiratory complications or post-procedural advanced healthcare utilisation.

Simultaneous quantification of total flavonoids and phenolic content in raw peanut seeds via NIR spectroscopy coupled with integrated algorithms.

Peanuts are nutritionally valuable for both humans and animals due to their high content of flavonoids and phenolic compounds. Herein, we explored the potential of near-infrared (NIR) spectroscopy coupled with efficient variable selection algorithms for quantitative prediction of total flavonoids (TFC) and total phenolics content (TPC) in raw peanut seeds. Spectrophotometrically, the reference results of the extracts for TFC and TPC were analysed and recorded. The integrated application of the synergy interval coupled competitive adaptive reweighted sampling-partial least squares (Si-CARS-PLS) were used for prediction. The model performance appraisal was based on the correlation coefficients of prediction (Rp), root mean square error of prediction (RMSEP), and residual predictive deviation (RPD). The Si-CARS-PLS performed optimally for TFC (Rp = 0.9137, RPD = 2.49) and TPC (Rp = 0.9042, RPD = 2.31), respectively. Moreover, the model (Si-CARS-PLS) was found to have an acceptable fit for the analytes under study since it achieved 0.88 for TFC and 0.86 for TPC based on the external validation. Therefore, these results showed that NIR coupled with Si-CARS-PLS could be used for the quantitative prediction of flavonoids and phenolic contents in raw peanut seeds.

Biological activities and wound healing potential of a water-soluble polysaccharide isolated from Glycyrrhiza glabra in Wistar rat.

The present study aimed to evaluate the in vitro antibacterial and antioxidant activities and the in vivo wound healing performance of a polysaccharide isolated from Glycyrrhiza glabra named PSG. It was structurally characterized by Fourier transformed infrared (FT-IR) spectroscopy, which confirmed the presence of different polysaccharides functional bands. The antioxidant capacity of PSG was determined in vitro and evaluated in vivo through the examination of wound healing capacity. Thirty two rats were randomly divided into four groups: group I was treated with physiological serum (negative control); group II was treated with "CYTOL CENTELLA®"; group III was treated with glycerol and group IV was treated with polysaccharide. The response to treatments was assessed by macroscopic, histologic, and biochemical parameters. Data revealed that our sample exhibited potential antioxidant activities and accelerated significantly the wound healing process, after ten days of treatment, proved by the higher wound appearance scores and a higher content of collagen confirmed by histological examination, when compared with control and "CYTOL CENTELLA®". Overall, these findings proved that this polysaccharide isolated from Glycyrrhiza glabra could be considered as a natural bioactive polymer for therapeutic process in wound healing applications.