Effect of acid etching on the micro-shear bond strength of resin composite-calcium silicate interface evaluated over different time intervals of bond aging.

INTRODUCTION: With the introduction of calcium silicate materials, pulp capping procedure has been simplified due to their therapeutic pulp capping capabilities and ability to be used as a provisional bulk restorative material simultaneously. The quality of the adhesive bond between these cements and composite is of clinical significance with regard to the longevity of the final laminate restoration. Thus, this study aims to compare the in vitro microtensile bond strengths of resin composite to different calcium silicate cements versus glass ionomer cement (GIC). MATERIALS AND METHODOLOGY: Resin composite was bonded to standardized disks of Biodentine, mineral trioxide aggregate (MTA), and GIC. The samples were divided into the following three groups, and each group was further divided into two subgroups based on the aging time (1 and 2 weeks) of the samples: (1) Group 1 - phosphoric acid etching on Biodentine. (2) Group 2 - phosphoric acid etching on MTA. (3) Group 3 - phosphoric acid etching on GIC. After bond aging, the microtensile bond strength was analyzed using the Instron universal testing machine. RESULTS: There was no significant difference in micro-SBS between 1- and 4-week bond aging of MTA and Biodentine group, while a significant decrease is seen in the GIC group. CONCLUSION: Calcium silicate cements are weak in the early setting phase. Thus, placing the overlying composite is best delayed for at least 2 weeks to allow adequate maturation of the material.

Evaluation of Biocompatibility and Osteogenic Potential of Tricalcium Silicate-based Cements Using Human Bone Marrow-derived Mesenchymal Stem Cells.

INTRODUCTION: The success of endodontic regeneration lies in the appropriate combination of stem cells and bioactive materials. Several novel dental materials are available on the market in this regard. Hence, the current study aimed to evaluate the proliferation, differentiation, and osteogenic potential of human bone marrow-derived mesenchymal stem cells (hBMSCs) onto biomaterials like ProRoot MTA (MTA; Dentsply Tulsa Dental, Tulsa, OK), Biodentine (BD; Septodont, Saint Maur de Fosses, France), and EndoSequence Root Repair Material (ERRM; Brasseler USA, Savannah, GA). METHODS: Dental cements were formulated into discs and assessed for their biocompatibility. hBMSCs were used to study biocompatitibility and the proliferative and osteogenic potential of these dental cements. A live dead assay was performed using confocal microscopy to study the biocompatibility, proliferation, and cell attachment property of the cements. An 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay was also performed on days 1, 3, 5, and 7 to study growth kinetics. The osteogenic potential of these cements was studied by inducing hBMSCs over them using osteogenic differentiation medium (assessed by alkaline phosphatase assay). RESULTS: ERRM and MTA have shown the best biocompatibility among the tricalcium silicate materials used with no significant difference between them. Both have shown significantly higher osteogenic bioactivity than BD. All 3 tricalcium silicate cements support good adherence of hBMSCs. CONCLUSIONS: All of the dental cements used in this study are biocompatible with the potential to induce proliferation and osteogenic differentiation of hBMSCs. Therefore, the newly introduced ERRM can be the material of choice in various endodontic applications.

Infiltrating lipomatosis of the face: A case series.

Infiltrating lipomatosis of the face is a very rare entity which is characterized by the collection of non-encapsulated mature adipocytes infiltrating local tissues, resulting in craniofacial deformities. Psychomotor development of the patients is normal, esthetics often being the primary concern to seek treatment. The presentation is always unilateral with hypertrophy of hard and soft structures on the affected side of the face. The pathogenesis of the condition is unclear. This condition shows a wide phenotypic range, uncertain prognosis with high rates of recurrence after surgery, and variable post-op cosmetic improvement. The condition shows no gender predilection, with most of the cases presenting in and beyond the second decade of life. Here, we present a series of four cases presenting in varying age groups with history of recurrence in three cases.