Increased resistance to pod shatter is associated with changes in the vascular structure in pods of a resynthesized Brassica napus line.

The architecture of the pod wall and dehiscence zone (DZ) was studied in populations of a resynthesized, shatter-resistant, oilseed rape line, DK142, and the commercial cultivar Apex. The dimensions of the pod wall and its component tissues were significantly larger in DK142. However, the variation in the pod architecture of Apex, DK142 and F2 populations derived from crosses of DK142 and Apex was found to have little or no role in pod shatter. By contrast, variation in the dimensions of the DZ characters correlated strongly and positively with shatter resistance. The size of the main vascular bundle (MVBV) of DK142 as it exited the valve and joined the vascular tissue of the replum was, on average, 60% larger than in Apex, the DZ was 40% wider and there was a high preponderance of vascular tissue other than the MVBV. The variation in the size of the MVBV accounted for much of the variation in shatter resistance of all populations, including shatter-susceptible Apex. The DZ width was also found to be important in explaining the limited range of shatter values in Apex, but in populations of DK142 and F2, where the amount of vascular intrusion into the DZ was much greater, the variation in DZ width was not important. The importance of the vascular tissue to shatter resistance was further highlighted by a novel microfracture test (MFT). By contrast, no significant difference between DK142 and Apex in the ease of separation of the thin-walled DZ cells was detected using the MFT.

Anoxia tolerance in the aquatic monocot Potamogeton pectinatus absence of oxygen stimulates elongation in association with an unusually large pasteur effect.

Elongation by stems of overwintered tubers of Potamogeton pectinatus (L.) is strongly promoted over several days by oxygen-free conditions. Characteristics of the respiration underpinning this unusual response were examined. Anaerobic plants produced ethanol and CO(2) in approximately equimolar amounts, indicating that glycolysis coupled to alcoholic fermentation was the principal CO(2)-producing respiratory pathway. Rates of CO(2) evolution by aerobic and anaerobic whole plants (shoot and tuber) were similar, suggesting a rate of glycolysis three times that of aerobic plants, i.e. a strong Pasteur effect. In the shoot alone, anaerobic CO(2) production was twice the aerobic rate indicating a 6-fold increase in the rate of glycolysis in this tissue. Anoxic stems contained more sucrose at a stronger concentration than slower-growing aerobic stems or anaerobic leaves, demonstrating that sugar supply to the site of most rapid growth exceeded demand in the absence of oxygen. Concentrations of potentially toxic acetaldehyde in the external medium were small (approximately 0.2 mol m(-3)) during anoxia and on return to aerated conditions. Lactic acid was undetectable under anaerobic conditions and in vivo (31)P-NMR analysis of shoots revealed a cytoplasmic acidification of only

Potamogeton pectinatus Is Constitutively Incapable of Synthesizing Ethylene and Lacks 1-Aminocyclopropane-1-Carboxylic Acid Oxidase.

A highly sensitive laser-driven photoacoustic detector responsive to [less than or equal to]2.1 nmol m-3 ethylene (50 parts per trillion [v/v]) was used for ethylene analysis. Dark-grown plants of Potamogeton pectinatus L. growing from small tubers made no ethylene. Exposure of shoots to white light, wounding, submergence in water followed by desubmergence, partial oxygen shortage, indole acetic acid, or carbon dioxide failed to induce ethylene production, although clear effects were observed in Pisum sativum L. Some ethylene was released after applying high concentrations of the ethylene precursor 1-aminocyclopropane-1-carboxylic acid (ACC; 10 mol m-3) to P. pectinatus, but the amount was trivial compared with that released by P. sativum. More endogenous ACC was found in P. pectinatus than in P. sativum. Considerable ACC oxidase activity was present in tissue extracts of P. sativum. However, no ACC oxidase activity was found in P. pectinatus, indicating that this is where ethylene production is arrested.

Analysis of the Journal of The American Dietetic Association, 1967 to 1983.

Analysis of the Journal of The American Dietetic Association was undertaken for 1967 to 1983 to determine whether there were changes that might have resulted from the report of the 1972 Study Commission on Dietetics. The study analyzed such factors as the number of original articles, educational background of authors and Journal advisory board members, abstracting of other Journal articles, indexing, citation analysis, and impact factor of the Journal. Study results show increased educational qualifications of authors and advisory board members, more original articles, more efficient indexing, and greater citation frequency.

Effects of progesterone on mammary carcinogenesis by DMBA applied directly to rat mammae.

The effects and site(s) of action of progesterone on DMBA mammary carcinogenesis in the rat, when a small dose of the carcinogen was applied directly to the inguinal mammary gland, were investigated. No reduction in tumour yield was apparent when progesterone was administered s.c. for 18 days before dusting DMBA. This finding contrasts with a previously reported inhibitory effect on carcinogenesis when hormone treatment was followed by intragastric administration of DMBA. When progesterone injections were begun either 2 days before or 2 days after direct application of DMBA, and were continued until the end of the experiment (135 or 195 days) an enhancement in carcinogenesis was observed similar to that previously demonstrated after gastric intubation of DMBA. These findings, together with previously reported observations, suggest that progesterone may exert its inhibitory effect on carcinogenesis by acting at a site outside the breast, perhaps on the liver. However, it is likely that the hormone acts directly on the mammary tissue to exert its enhancing effect on tumorigenesis.

Pulmonary alveolar proteinosis. Review of the literature with follow-up studies and report of two new cases.

In the period from 1958, when Rosen and coworkers first reported a condition in which the pulmonary alveoli are filled with an eosinophilic material, to the beginning of 1964, reports of 93 cases had accumulated in the world literature, including two cases included herein. The cause of this disease, which Rosen called "pulmonary alveolar proteinosis," is not known, nor is there a known means of cure. The usual patient is a white man between 30 and 50 years of age who may do any kind of work. The first symptoms may be those of pulmonary infection or pulmonary insufficiency. Patients with pulmonary alveolar proteinosis are prone to nocardiosis and infection with other fungi. Diagnosis is made by lung biopsy. Twenty of the 93 patients reported upon were alive at the time of this review, 37 were dead and 36 had been lost to follow-up.