RESUMO
Spices contain plenty of bioactive compounds, used to valorize foods. However, product quality may be affected by contaminations and adulterations along the global production chain. A newly developed multi-imaging in combination with bioactivity screening directly pointed to individual multi-potent compounds. For cinnamon as prominent example, the multi-imaging results provided a wealth of new information on their effects and clearly visualized the valorizing potential of cinnamon to foods. The separation focus was in the mid-polar to apolar range. Eight effect-directed assays (EDA, i.e. one radical scavenging, two biological and five biochemical assays) were performed in situ the high-performance thin-layer chromatography (HPTLC) adsorbent. Several multi-potent compound zones were revealed and further characterized by high-resolution mass spectrometry (HRMS), highlighting the bioactive potential of cinnamaldehyde, cinnamic acid, benzoic acid, coumarin, linoleic acid, oleic acid, stearic acid, palmitic acid, caproic acid, and linalool oxide. This HPTLC-UV/Vis/FLD-EDA-HRMS profiling provided comprehensive information on product quality and safety.
