Advancements of CRISPR technology in public health-related analysis.

Pathogens and contaminants in food and the environment present significant challenges to human health, necessitating highly sensitive and specific diagnostic methods. Traditional approaches often struggle to meet these requirements. However, the emergence of the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) system has revolutionized nucleic acid diagnostics. The present review provides a comprehensive overview of the biological sensing technology based on the CRISPR/Cas system and its potential applications in public health-related analysis. Additionally, it explores the enzymatic cleavage capabilities mediated by Cas proteins, highlighting the promising prospects of CRISPR technology in addressing bioanalysis challenges. We discuss commonly used CRISPR-Cas proteins and elaborate on their application in detecting foodborne bacteria, viruses, toxins, other chemical pollution, and drug-resistant bacteria. Furthermore, we highlight the advantages of CRISPR-based sensors in the field of public health-related analysis and propose that integrating CRISPR-Cas biosensing technology with other technologies could facilitate the development of more diverse detection platforms, thereby indicating promising prospects in this field.

The effect of comfort care based on the collaborative care model on the compliance and self-care ability of patients with coronary heart disease.

BACKGROUND: Coronary heart disease (CHD) causes mental discomfort in patients, so there is a pressing need to strengthen the nursing cooperation with patients during treatment, which can help patients to regulate their psychological status, promoting successful rehabilitation. This study was to explore the effect of comfort care based on the collaborative care model (CCM) on the compliance and self-care ability of patients with CHD. METHODS: A total of 104 CHD patients were randomly selected in our hospital between April 2019 and April 2020. They were divided into two groups (with 52 cases in each group) using a random number table. Routine care was applied in the control group, while comfort care based on the CCM was employed in the study group. Self-made questionnaires were used to investigate the health knowledge proficiency, psychological status, compliance, self-care ability, and comfort degree in the two groups before and after intervention. RESULTS: Following the interventions, the health knowledge proficiency in the study group was higher than that in the control group (P<0.05). The Self-rating Anxiety Scale (SAS) and Self-rating Depression Scale (SDS) scores in the study group were lower than those in the control group (P<0.05). The compliance rate in the study group was 96.15%, which was higher than that of the control group (82.69%) (P<0.05). The Exercise of Self-care Agency (ESCA) score in the study group was also higher than that of the control group (P<0.05). Moreover, the physical, mental, social, and emotional comfort scores in the study group were higher than those in the control group (P<0.05). CONCLUSIONS: Comfort nursing based on the CCM improves CHD patients' health knowledge, regulates their psychological status, and improves their compliance, self-care ability, and comfort.