RESUMO
High molecular weight glutenin subunits (HMW-GS) play an essential role in wheat processing quality. In this study, we evaluated the genetic pattern with HMW-GS composition between generations and examined whether agronomic and quality traits were correlated with each other. A wheat (Triticum aestivum L.) cultivar with high protein content and 2 cultivars with low protein content were subjected to a reciprocal cross. Sixteen agronomic and 4 quality characteristics were investigated. A total of 216 seeds from each F2 generation were chosen randomly and analyzed for HMW-GS composition using sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Agronomic and quality characteristics were not significantly different between reciprocal crosses, indicating no cytoplasmic effect on the characteristics studied. The separation ratio of 2 HMW-GS loci was 9:3:3:1, indicating no linkage between any 2 loci. The novel HMW-GS N was detected in cultivar R145, which did not follow the Mendelian segregation ratio. A Glu-A1a(1) band was not detected in 1 individual from Tian8901xR145. Average grain weight per spike was significantly correlated with quality characteristics and may be a suitable criterion for selecting high protein content in wheat breeding programs.
Assuntos
Proteínas de Plantas/genética , Triticum/genética , Eletroforese em Gel de Poliacrilamida , Glutens/química , Glutens/genética , Peso Molecular , Fenótipo , Melhoramento Vegetal , Proteínas de Plantas/química , Subunidades Proteicas/química , Subunidades Proteicas/genética , Sementes/genética , Triticum/químicaRESUMO
Dickkopf-1 (DKK-1) is a secreted protein that inhibits Wnt signaling. However, the clinical significance and prognostic value of serum DKK-1 levels have not been previously investigated in bladder cancer in Chinese patients. Blood samples were taken from 94 consecutive patients diagnosed with bladder cancer and 60 healthy control subjects. Serum DKK-1 expression levels were measured by enzyme-linked immunosorbent assay according to the manufacturer's directions. The Kaplan-Meier method was used to estimate survival, and the log-rank test was used to test differences between the survival curves. Multivariate survival analysis was performed for all parameters deemed significant in the univariate analyses using the Cox regression model. The mean serum DKK-1 level in patients with bladder cancer was 35.91 ± 16.09 ng/mL, which was significantly higher than that in healthy individuals (9.08 ± 5.21 ng/mL, P < 0.001). Furthermore, serum DKK-1 levels were correlated significantly with lymph node metastasis (P = 0.021), distant metastasis (P = 0.013), and TNM stage (P = 0.008). Kaplan-Meier analysis using the log-rank test indicated that high serum DKK-1 levels were linked to poorer survival (33.4 vs 70.1%; P = 0.007). Multivariate analysis revealed that serum DKK-1 levels represented an independent prognostic factor for overall survival (hazard ratio = 2.365, 95% confidence interval = 1.873-8.881, P = 0.029). High serum DKK-1 levels may be associated with tumor progression and poor prognosis in bladder cancer and may be used as a potential biomarker to predict the prognosis of patients with bladder cancer.
Assuntos
Biomarcadores Tumorais/sangue , Peptídeos e Proteínas de Sinalização Intercelular/sangue , Prognóstico , Neoplasias da Bexiga Urinária/sangue , Adulto , Idoso , Feminino , Regulação Neoplásica da Expressão Gênica , Humanos , Estimativa de Kaplan-Meier , Masculino , Pessoa de Meia-Idade , Modelos de Riscos Proporcionais , Neoplasias da Bexiga Urinária/genética , Neoplasias da Bexiga Urinária/patologia , Via de Sinalização WntRESUMO
The isolation and characterization of a human T-cell lymphotropic retrovirus related to human T-cell lymphotropic virus type I (HTLV-I) from cerebrospinal fluid of a Jamaican patient with tropical spastic paraparesis is described. The virus isolate is a typical type C retrovirus as seen by electron microscopy and is related to prototype HTLV-I isolated from patients with adult T-cell leukemia but is not identical to this prototype HTLV-I as seen by restriction enzyme mapping.
Assuntos
Líquido Cefalorraquidiano/microbiologia , Vírus Linfotrópico T Tipo 1 Humano/isolamento & purificação , Paraparesia Espástica Tropical/microbiologia , Idoso , Células Cultivadas , Enzimas de Restrição do DNA , DNA Viral/análise , Anticorpos Antideltaretrovirus/análise , Feminino , Imunofluorescência , Vírus Linfotrópico T Tipo 1 Humano/enzimologia , Vírus Linfotrópico T Tipo 1 Humano/genética , Humanos , Jamaica , Leucemia de Células T/microbiologia , Leucócitos Mononucleares/microbiologia , Microscopia Eletrônica , Paraparesia Espástica Tropical/imunologia , DNA Polimerase Dirigida por RNA/análiseRESUMO
The isolation and characterization of a human T-cell lymphotrophic virus type I (HTLV-I) from cerebrospinal fluid of a Jamaican patient with tropical spastic paraparesis is described. The virus isolate is a typical type C retrovirus as seen by electron microscopy and is related to prototype HTLV-I isolated from patients with adult T-cell leukemia but is not identical to this prototype HTLV-I as seen by restriction enzyme mapping.(AU)