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1.
Turk Neurosurg ; 33(6): 929-935, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-34374979

RESUMO

AIM: To investigate the safety and efficacy of endoport-assisted endoscopic techniques for removing intraventricular lesions. MATERIAL AND METHODS: Data of patients with intraventricular lesions who were surgically treated by endoport-assisted endoscopic resection between January 2018 and February 2019 were retrospectively reviewed. The surgical procedures, complications and outcomes were analyzed. RESULTS: A total of 11 patients, with a mean age of 33 years (5-70 years) were included in the study. The mean Karnofsky Performance Scale (KPS) score evaluated on admission was 50.0 ± 7.0. Lesions located in the unilateral ventricle, the third ventricle and multiple sites of ventricles were recorded in 7, 2 and 2 patients, respectively. The average lesion size was 3.4 ± 0.4 cm (2-6 cm). Gross-total removal of all lesions was achieved, and all patients experienced a stable recovery after operations except for one hemorrhage and one visual field defect occurring in two patients in the early postoperative period. With a follow-up of 6-19 months, dysfunctions and complications occurring pre- or postoperation gradually recovered to different degrees. The mean KPS score was 85.5 ± 4.3 at the last follow-up, and no tumor recurrence was observed in any of the patients. CONCLUSION: Endoport-assisted endoscopic techniques could be a simple, minimally invasive surgical method in the resection of lesions located in the lateral ventricle, the third ventricle, or both with acceptable surgical complications occurring in patients.


Assuntos
Neuroendoscopia , Humanos , Adulto , Estudos Retrospectivos , Resultado do Tratamento , Neuroendoscopia/métodos , Procedimentos Neurocirúrgicos/métodos , Ventrículos Cerebrais/cirurgia
2.
J Neuroimmunol ; 298: 153-9, 2016 09 15.
Artigo em Inglês | MEDLINE | ID: mdl-27609289

RESUMO

Mesial temporal lobe epilepsy (MTLE) is the most common form of focal epilepsies in adults and proinflammatory cytokines have long been thought to play an important role in pathogenesis and epileptogenicity. In the present study, we investigated the levels and expression patterns of the interleukin 17 (IL-17) system in temporal neocortex and hippocampus from 24 patients with MTLE and 8 control (Ctr) samples. We found that IL-17 and IL-17 receptor (IL-17R) were clearly upregulated in MTLE at both mRNA and protein levels, compared with Ctr. Immunostaining indicated that neurons, astrocytes, microglia and endothelial cells of blood vessels are the major sources of IL-17. These findings suggest that IL-17 system may be involved in the pathogenesis and epileptogenicity of MTLE.


Assuntos
Córtex Cerebral/metabolismo , Epilepsia do Lobo Temporal/patologia , Hipocampo/metabolismo , Interleucina-17/metabolismo , Receptores de Interleucina-7/metabolismo , Adolescente , Adulto , Análise de Variância , Contagem de Células , Epilepsia do Lobo Temporal/metabolismo , Feminino , Humanos , Interleucina-17/genética , Masculino , RNA Mensageiro/metabolismo , Receptores de Interleucina-7/genética , Adulto Jovem
3.
J Neuropathol Exp Neurol ; 75(8): 718-730, 2016 08.
Artigo em Inglês | MEDLINE | ID: mdl-27288906

RESUMO

Focal cortical dysplasia (FCD) likely results from abnormal migration of neural progenitor cells originating from the subventricular zone. To elucidate the roles in molecules that are involved in neural migration pathway abnormalities in FCDs, we investigated the expression patterns of transient receptor potential canonical channel 6 (TRPC6) and brain-derived neurotrophic factor (BDNF) in cortical lesions from FCD patients and in samples of normal control cortex. TRPC6 and BDNF mRNA and protein levels were increased in FCD lesions. By immunohistochemistry, they were strongly expressed in microcolumns, heterotopic neurons, dysmorphic neurons, and balloon cells (BCs). Colocalization assays revealed that most of the misshapen TRPC6-positive or heterotopic cells had a neuronal lineage with the exception of TRPC6-positive FCDiib patient BCs, which had both neuronal and glial features. Most TRPC6-positive cells were glutamatergic neurons. There was also greater expression of calmodulin-dependent kinase IV (CaMKIV), the downstream factor of TRPC6, in FCD lesions, suggesting that TRPC6 expression promoted dendritic growth and the development of dendritic spines and excitatory synapses via the CaMKIV-CREB pathway in FCD. Thus, overexpression of BDNF and TRPC6 and activation of the TRPC6 signal transduction pathway in cortical lesions of FCD patients may contribute to FC pathogenesis and epileptogenesis.

4.
J Neuroinflammation ; 13(1): 85, 2016 Apr 19.
Artigo em Inglês | MEDLINE | ID: mdl-27095555

RESUMO

BACKGROUND: Focal cortical dysplasia type IIb (FCD IIb) and tuberous sclerosis complex (TSC) are well-recognized causes of chronic intractable epilepsy in children. Accumulating evidence suggests that activation of the microglia/macrophage and concomitant inflammatory response in FCD IIb and TSC may contribute to the initiation and recurrence of seizures. The membrane glycoproteins CD47 and CD200, which are highly expressed in neurons and other cells, mediate inhibitory signals through their receptors, signal regulatory protein α (SIRP-α) and CD200R, respectively, in microglia/macrophages. We investigate the levels and expression pattern of CD47/SIRP-α and CD200/CD200R in surgically resected brain tissues from patients with FCD IIb and TSC, and the potential effect of soluble human CD47 Fc and CD200 Fc on the inhibition of several proinflammatory cytokines associated with FCD IIb and TSC in living epileptogenic brain slices in vitro. The level of interleukin-4 (IL-4), a modulator of CD200, was also investigated. METHODS: Twelve FCD IIb (range 1.8-9.5 years), 13 TSC (range 1.5-10 years) patients, and 6 control cases (range 1.5-11 years) were enrolled. The levels of CD47/SIRP-α and CD200/CD200R were assessed by quantitative real-time polymerase chain reaction and western blot. The expression pattern of CD47/SIRP-α and CD200/CD200R was investigated by immunohistochemical analysis, and the cytokine concentrations were measured by enzyme-linked immune-sorbent assays. RESULTS: Both the messenger RNA and protein levels of CD47, SIRP-α, and CD200, as well as the mRNA level of IL-4, were downregulated in epileptogenic lesions of FCD IIb and TSC compared with the control specimens, whereas CD200R levels were not significantly changed. CD47, SIRP-α, and CD200 were decreasingly expressed in dysmorphic neuron, balloon cells, and giant cells. CD47 Fc and CD200 Fc could inhibit IL-6 release but did not suppress IL-1ß or IL-17 production. CONCLUSIONS: Our results suggest that microglial activation may be partially caused by CD47/SIRP-α- and CD200/CD200R-mediated reductions in the immune inhibitory pathways within FCD IIb and TSC cortical lesions where chronic neuroinflammation has been established. Upregulation or activation of CD47/SIRP-α and CD200/CD200R may have therapeutic potential for controlling neuroinflammation in human FCD IIb and TSC.


Assuntos
Antígenos CD/biossíntese , Encéfalo/metabolismo , Antígeno CD47/biossíntese , Epilepsia/metabolismo , Malformações do Desenvolvimento Cortical do Grupo I/metabolismo , Esclerose Tuberosa/metabolismo , Western Blotting , Criança , Pré-Escolar , Regulação para Baixo , Ensaio de Imunoadsorção Enzimática , Feminino , Humanos , Imuno-Histoquímica , Lactente , Masculino , Microglia/metabolismo , Neurônios/metabolismo , Reação em Cadeia da Polimerase em Tempo Real
5.
CNS Neurosci Ther ; 22(4): 280-90, 2016 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-26842013

RESUMO

AIM: Focal cortical dysplasia (FCD) represents a well-known cause of medically intractable epilepsy. Studies found that transient receptor potential vanilloid receptor 4 (TRPV4) may participate in the occurrence of seizures. This study investigated the expression patterns of TRPV4 in FCD and the cascade that regulate functional state of TRPV4 in cortical neurons. METHODS: Thirty-nine surgical specimens from FCD patients and 10 age-matched control samples from autopsies were included in this study. Protein expression and distribution were detected by Western blot, immunohistochemistry, and immunofluorescence staining. Calcium imaging was used to detect the TRPV4-mediated Ca(2+) influx in cortical neurons. RESULTS: (1) The protein levels of TRPV4 and of an upstream factor, protein kinase C (PKC), were markedly elevated in FCD. (2) TRPV4 staining was stronger in the dysplastic cortices of FCD and mainly observed in neuronal microcolumns and malformed cells. (3) The activation of TRPV4 was central for [Ca(2+)]i elevation in cortical neurons, and this activity of TRPV4 in cortical neurons was regulated by the PKC, but not the PKA, pathway. CONCLUSION: The overexpression and altered cellular distribution of TRPV4 in FCD suggest that TRPV4 may potentially contribute to the epileptogenesis of FCD.


Assuntos
Malformações do Desenvolvimento Cortical/metabolismo , Canais de Cátion TRPV/metabolismo , Adolescente , Adulto , Animais , Cálcio/metabolismo , Criança , Pré-Escolar , Proteínas Quinases Dependentes de AMP Cíclico/metabolismo , Epilepsia/etiologia , Epilepsia/metabolismo , Epilepsia/patologia , Epilepsia/cirurgia , Feminino , Humanos , Lactente , Masculino , Malformações do Desenvolvimento Cortical/complicações , Malformações do Desenvolvimento Cortical/patologia , Malformações do Desenvolvimento Cortical/cirurgia , Neurônios/metabolismo , Neurônios/patologia , Proteína Quinase C/metabolismo , Ratos Sprague-Dawley , Adulto Jovem
6.
J Mol Neurosci ; 59(2): 241-50, 2016 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-26798047

RESUMO

Mesial temporal lobe epilepsy (MTLE) is a frequent form of focal intractable epilepsy in adults. We previously reported overexpression of vascular endothelial growth factor C (VEGF-C) and its receptors, VEGFR-2 and VEGFR-3, in epilepsy-associated tuberous sclerosis complex. To identify whether VEGF-C and its receptors are involved in epileptogenesis of MTLE, we investigated the levels and expression pattern of VEGF-C and its receptors in temporal neocortex and hippocampus (HPC) from 28 patients with MTLE and ten control (CTX) subjects. Real-time quantitative polymerase chain reaction and Western blotting results revealed upregulated mRNA and immunoreactive protein levels of VEGF-C, VEGFR-2, and VEGFR-3 in the MTLE group compared to the control groups. Immunohistochemistry and double-labeled immunofluorescence showed that VEGF-C was highly expressed in neurons and astrocytes, including reactive astrocytes and vascular endothelial cells, VEGFR-2 was expressed at a high level in reactive astrocytes and vascular endothelial cells, but not in neurons, whereas VEGFR-3 was only overexpressed in reactive astrocytes. Taken together, these findings suggest that VEGF-C and its receptors, VEGFR-2 and VEGFR-3, may contribute to the epileptogenesis of MTLE.


Assuntos
Epilepsia do Lobo Temporal/metabolismo , Fator C de Crescimento do Endotélio Vascular/metabolismo , Receptor 2 de Fatores de Crescimento do Endotélio Vascular/metabolismo , Receptor 3 de Fatores de Crescimento do Endotélio Vascular/metabolismo , Adolescente , Adulto , Astrócitos/metabolismo , Estudos de Casos e Controles , Epilepsia do Lobo Temporal/genética , Feminino , Hipocampo/citologia , Hipocampo/metabolismo , Humanos , Masculino , Neocórtex/citologia , Neocórtex/metabolismo , Neurônios/metabolismo , Fator C de Crescimento do Endotélio Vascular/genética , Receptor 2 de Fatores de Crescimento do Endotélio Vascular/genética , Receptor 3 de Fatores de Crescimento do Endotélio Vascular/genética
7.
J Neuropathol Exp Neurol ; 75(1): 61-8, 2016 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-26671983

RESUMO

Focal cortical dysplasias (FCDs) are major brain malformations that commonly lead to medically intractable epilepsy. The purinergic ionotropic P2X7 receptor (P2X7R) is an atypical P2X subtype that gates calcium and sodium ions. Previous animal studies have suggested that P2X7R is a contributing factor in epileptogenesis. This study aimed to define the distribution and expression of P2X7R in 35 FCD patient-surgical-resection specimens relative to autopsy control samples (n = 8). Immunohistochemical colocalization assays revealed that P2X7R was primarily expressed in neurons, astrocytes, and microglia. In FCD samples, P2X7R protein levels were increased in abnormal cell types such as dysmorphic neurons and balloon cells, which are characteristic of FCD. By real-time PCR and Western blotting, P2X7R mRNA and protein expression levels were elevated in FCD patient samples vs control samples; P2X7R expression was also higher in FCDII vs FCDIa patient samples. Because interleukin-1ß is a downstream factor of the P2X7R signaling pathway, we determined that there was also moderate-to-strong interleukin-1ß expression in the dysmorphic neurons, balloon cells, and microglia in FCD patient lesions. These results indicate that increasing P2X7R levels may contribute to the pathogenesis of human FCD and that P2X7R represents a potential anti-epileptogenic target.


Assuntos
Córtex Cerebral/química , Córtex Cerebral/metabolismo , Malformações do Desenvolvimento Cortical/metabolismo , Receptores Purinérgicos P2X7/análise , Receptores Purinérgicos P2X7/biossíntese , Adolescente , Córtex Cerebral/patologia , Criança , Pré-Escolar , Feminino , Regulação da Expressão Gênica , Humanos , Lactente , Masculino , Malformações do Desenvolvimento Cortical/diagnóstico , Malformações do Desenvolvimento Cortical/genética , Receptores Purinérgicos P2X7/genética , Adulto Jovem
8.
J Neuropathol Exp Neurol ; 73(3): 206-22, 2014 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-24487795

RESUMO

Focal cortical dysplasia (FCD) is a well-known cause of medically intractable epilepsy. To understand the potential role of the inflammatory cytokine interleukin 2 (IL-2) in the pathogenesis of FCD, we investigated the expression patterns of IL-2 and its receptors (IL-2Rs) in FCD and control samples that included epileptic neocortex from mesial temporal lobe epilepsy patients and nonepileptic normal cortex (CTX). Greater mRNA and protein levels of IL-2 and IL-2Rs were observed in FCD versus CTX samples. Moreover, the expression of IL-2 and IL-2Rs was significantly higher in FCD II than FCD I. In situ hybridization and immunohistochemistry results indicated that IL-2 and IL-2Rs were strongly expressed in hypertrophic neurons and neuronal microcolumns in FCD I and highly expressed in malformed cells in FCD II. In addition, the protein levels of Janus kinase 1, Janus kinase 3, phosphorylated signal transducer and activator of transcription 5, which are important downstream factors in the IL-2 signaling pathway, were increased in FCD lesions. Soluble IL-2R was decreased in FCD compared with that in CTX samples. These results suggest that upregulation of IL-2 and IL-2Rs combined with activation of IL-2-dependent signaling pathways may contribute to the pathogenesis of FCD.


Assuntos
Córtex Cerebral/metabolismo , Interleucina-2/metabolismo , Malformações do Desenvolvimento Cortical/patologia , Receptores de Interleucina-2/metabolismo , Transdução de Sinais/fisiologia , Adolescente , Contagem de Células , Córtex Cerebral/patologia , Criança , Pré-Escolar , Ensaio de Imunoadsorção Enzimática , Epilepsia do Lobo Temporal/etiologia , Epilepsia do Lobo Temporal/patologia , Feminino , Regulação da Expressão Gênica , Humanos , Lactente , Interleucina-2/genética , Janus Quinases/genética , Janus Quinases/metabolismo , Masculino , Malformações do Desenvolvimento Cortical/complicações , RNA Mensageiro/metabolismo , Receptores de Interleucina-2/genética , Fator de Transcrição STAT5/genética , Fator de Transcrição STAT5/metabolismo
9.
J Mol Neurosci ; 49(1): 182-93, 2013 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-22936245

RESUMO

Transient receptor potential vanilloid type-1 (TRPV1) is a ligand-gated nonselective cation channel that has been well characterized in peripheral pain pathway. Recent evidence from animal models of temporal lobe epilepsy (TLE) has supported the important role of TRPV1 in epileptogenesis. In this study, we investigated the expression and cellular distribution of TRPV1 in the temporal cortex (CTX) and hippocampus (HPC) from 26 patients with mesial TLE (MTLE) compared with 12 histologically normal samples. Reverse transcription-PCR and Western blotting revealed up-regulated mRNA and protein levels of TRPV1 in the MTLE group versus the control group. Immunohistochemistry data demonstrated that TRPV1 was mainly distributed in the cell bodies and dendrites of neurons. Double-labeled immunofluorescence further revealed that TRPV1 was localized on NeuN-positive neurons and GFAP-positive astrocytes, but not on HLA-positive microglia. In addition, its co-localization with glutamate and gamma-aminobutyric acid (GABA) indicated that TRPV1 was distributed on both glutamatergic and GABAergic neurons. Moreover, nerve growth factor, a sensitizing factor for TRPV1, was showed a higher expression pattern in MTLE patients. Taken together, our findings suggest that the overexpression and distribution patterns of TRPV1 might be involved in the pathogenesis and epileptogenesis of human MTLE.


Assuntos
Córtex Cerebral/metabolismo , Epilepsia do Lobo Temporal/congênito , Hipocampo/metabolismo , Canais de Cátion TRPV/genética , Regulação para Cima , Adolescente , Adulto , Astrócitos/metabolismo , Estudos de Casos e Controles , Córtex Cerebral/patologia , Dendritos/metabolismo , Epilepsia do Lobo Temporal/diagnóstico , Epilepsia do Lobo Temporal/genética , Feminino , Neurônios GABAérgicos/metabolismo , Ácido Glutâmico/metabolismo , Hipocampo/patologia , Humanos , Masculino , Microglia/metabolismo , Fator de Crescimento Neural/genética , Fator de Crescimento Neural/metabolismo , RNA Mensageiro/biossíntese , Canais de Cátion TRPV/metabolismo , Ácido gama-Aminobutírico/metabolismo
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