Th22 is the effector cell of thymosin ß15-induced hair regeneration in mice.

BACKGROUND: Thymosin beta family has a significant role in promoting hair regeneration, but which type of T cells play a key role in this process has not been deeply studied. This research aimed to find out the subtypes of T cell that play key role in hair regeneration mediated by thymosin beta 15 (Tß15). METHODS: Ready-to-use adenovirus expressing mouse Tmsb15b (thymosin beta 15 overexpression, Tß15 OX) and lentivirus-Tß15 short hairpin RNA (Tß15 sh) were used to evaluate the role of Tß15 in hair regeneration and development. The effect of Th22 cells on hair regeneration was further studied by optimized Th22-skewing condition medium and IL-22 binding protein (IL-22BP, an endogenous antagonist of IL-22, also known as IL-22RA2) in both ex vivo culture C57BL/6J mouse skin and BALB/c nude mice transplanted with thymus organoid model. RESULTS: The results show that Tß15, the homologous of Tß4, can promote hair regeneration by increasing the proliferation activity of hair follicle cells. In addition, high-level expression of Tß15 can not only increase the number of Th22 cells around hair follicles but also accelerate the transformation of hair follicles to maturity. Consistent with the expected results, when the IL-22BP inhibitor was used to interfere with Th22, the process of hair regeneration was blocked. CONCLUSIONS: In conclusion, Th22 is the key effector cell of Tß15 inducing hair regeneration. Both Tß15 and Th22 may be the potential drug targets for hair regeneration.

Uncovering the asymmetric impacts of economic policy uncertainty on green financial markets in China.

Green finance is considered a novel tool of financing to promote the development of the green economy, which has huge investment attractiveness. Previous studies detected the impacts of economic policy uncertainty (EPU) on the green financial markets are mixed. To this end, this study deeply investigates the asymmetric and heterogeneous impacts of EPU on the green bond and green stock markets based on the quantile-on-quantile (QQ) method. Using the data of China Economic Policy Uncertainty Index (CNEPU) and green financial indices, we get some interesting results. (1) EPU has an overall negative effect on the green financial markets, and the green stock market reacts more strongly than the green bond market. (2) For green bond market, the higher quantiles of EPU on a bear market have more significant effect than that on a bull market. (3) For green stock market, a negative effect of EPU on green stock market is observed for the lower quantiles of EPU, while a positive effect is noted at the highest quantiles of EPU. This paper could provide a reference for investors making green investment strategies and for policymakers making policies to promote green development.

Age-related hearing loss and its potential drug candidates: a systematic review.

BACKGROUND: Age-related hearing loss (ARHL) is one of the main illnesses afflicting the aged population and has a significant negative impact on society, economy, and health. However, there is presently no appropriate therapeutic treatment of ARHL due to the absence of comprehensive trials. OBJECTIVES: The goal of this review is to systematically evaluate and analyze recent statistics on the pathologic classifications, risk factors, treatment strategies, and drug candidates of ARHL, including that from traditional Chinese medicine (TCM), to provide potential new approaches for preventing and treating ARHL. METHODS: Literature related to ARHL was conducted in databases such as PubMed, WOS, China National Knowledge Infrastructure (CNKI), and Wanfang from the establishment of the database to Jan, 2023. The pathology, causal factor, pathophysiological mechanism, treatment strategy, and the drug candidate of ARHL were extracted and pooled for synthesis. RESULTS: Many hypotheses about the etiology of ARHL are based on genetic and environmental elements. Most of the current research on the pathology of ARHL focuses on oxidative damage, mitochondrial dysfunction, inflammation, cochlear blood flow, ion homeostasis, etc. In TCM, herbs belonging to the kidney, lung, and liver meridians exhibit good hearing protection. Seven herbs belonging to the kidney meridian, 9 belonging to the lung meridian, and 4 belonging to the liver meridian were ultimately retrieved in this review, such as Polygonum multiflorum Thunb., Panax ginseng C.A. Mey, and Pueraria lobata (Willd.) Ohwi. Their active compounds, 2,3,4',5-Tetrahydroxystilbene-2-O-D-glucoside, ginsenoside Rb1, and puerarin, may act as the molecular substance for their anti-ARHL efficacy, and show anti-oxidative, neuroprotective, anti-inflammatory, anti-apoptotic, or mitochondrial protective effects. CONCLUSION: Anti-oxidants, modulators of mitochondrial function, anti-inflammation agents, vasodilators, K+ channel openers, Ca2+ channel blockers, JNK inhibitors, and nerve growth factors/neurotrophic factors all contribute to hearing protection, and herbs are an important source of potential anti-ARHL drugs.

Thymosin α1 and Its Role in Viral Infectious Diseases: The Mechanism and Clinical Application.

Thymosin α1 (Tα1) is an immunostimulatory peptide that is commonly used as an immune enhancer in viral infectious diseases such as hepatitis B, hepatitis C, and acquired immune deficiency syndrome (AIDS). Tα1 can influence the functions of immune cells, such as T cells, B cells, macrophages, and natural killer cells, by interacting with various Toll-like receptors (TLRs). Generally, Tα1 can bind to TLR3/4/9 and activate downstream IRF3 and NF-κB signal pathways, thus promoting the proliferation and activation of target immune cells. Moreover, TLR2 and TLR7 are also associated with Tα1. TLR2/NF-κB, TLR2/p38MAPK, or TLR7/MyD88 signaling pathways are activated by Tα1 to promote the production of various cytokines, thereby enhancing the innate and adaptive immune responses. At present, there are many reports on the clinical application and pharmacological research of Tα1, but there is no systematic review to analyze its exact clinical efficacy in these viral infectious diseases via its modulation of immune function. This review offers an overview and discussion of the characteristics of Tα1, its immunomodulatory properties, the molecular mechanisms underlying its therapeutic effects, and its clinical applications in antiviral therapy.

The macrophage polarization in inflammatory dermatosis and its potential drug candidates.

Inflammatory dermatosis is characterized by persistent inflammatory infiltration and hard repair of diseased skin. As a member of the human innate immune cells, macrophages usually show different phenotypes in different diseases. The macrophage phenotype (M1/M2) imbalance caused by the increase of M1 macrophages or the decrease of M2 macrophages is common in inflammatory dermatosis. In recent years, with the deepening research on inflammatory skin diseases, more and more natural medicines/traditional Chinese medicines (TCMs), represented by Shikonin and Angelica Dahurica, have shown their therapeutic effects by affecting the polarization of macrophages. This review introduced macrophage polarization in different inflammatory dermatosis, such as psoriasis. Then summarized the natural medicines/TCMs that have potential therapeutic effects so far and introduced their mechanisms of action and the proteins/signal pathways involved. We found that the TCMs with therapeutic effects listed in this review are closely related to the theory of five flavors and four properties of Chinese medicinal, and most of them are bitter, acrid and sweet. Bitter TCMs have antipyretic, anti-inflammatory and antibacterial effects, which may improve the persistent inflammation of M1 macrophage infiltration. Acrid TCMs have the effect of promoting blood circulation, while sweet TCMs have the effect of nourishing. These 2 flavors may accelerate the repair of skin lesions of inflammatory dermatosis by affecting M2 macrophages. In conclusion, we hope to provide sufficient knowledge for natural medicine research and the development of inflammatory dermatosis related to macrophage phenotype imbalance.

Spillover Network Features from the Industry Chain View in Multi-Time Scales.

Financial stocks in the industry chain interact notably because of close economic and technical relationships. Some participants pay particular attention to one industry chain and are concerned with different investment horizons. The motivation for this study is to offer more targeted information to various market participants who focus on different time scales in one industry chain from a systematic perspective by combining the GARCH-BEKK, heterogeneous network, and wavelet analysis methods. The findings are as follows: (1) For parties who prefer to take more risks to gain higher returns, scale 2 (4-8 days) is a good option, while long-term investment (32-128 days) is suitable for conservative investors. (2) In most cases, some links in the industry chain are particularly sensitive to changes in stocks in other links. (3) The influence, sensitivity, and intermediary of stocks in the industry chain on different time scales were explored, and participants could use the resulting information to monitor the market or select stocks. (4) The structures, key players, and industry chain attributes of the main transmission paths differ on multi-time scales. Risk transmission can be controlled by intercepting important spillover relations within the paths.

Revisiting the Dynamic Response of Chinese Price Level to Crude Oil Price Shocks Based on a Network Analysis Method.

Crude oil price shocks have led to a fluctuation in commodity prices through the industrial chain and supply-demand relationships, which can substantially influence a country's economy. In this paper, we propose a transmission model of oil price shocks to Chinese price levels and explore the direct and indirect impacts of crude oil price shocks on various Chinese price indices, combining the Granger causality test, impulse response function, and network analysis method. The empirical data are the Brent, WTI, Dubai, and Daqing spot crude oil prices and eight categories of Chinese price indices from January 2011 to March 2020. We found the following results: (1) Consumer price index (CPI) and the price index for means of agricultural production (MAPPI) cannot be directly impacted by crude oil price fluctuations, while they could be indirectly affected. (2) The duration and degree of the impacts of oil prices on each price index vary, and the export price index (EPI) is the most significantly affected. (3) The proportion of the indirect impact in the total impact of crude oil price shocks ranges from 0.03% to 100.00%. Thus, indirect influence cannot be ignored when analyzing the influence of crude oil price fluctuation on Chinese price level.