APP1/NTL9-CalS8 module ensures proper phloem differentiation by stabilizing callose accumulation and symplastic communication.

Phloem sieve elements (PSE), the primary conduits collaborating with neighboring phloem pole pericycle (PPP) cells to facilitate unloading in Arabidopsis roots, undergo a series of developmental stages before achieving maturation and functionality. However, the mechanism that maintains the proper progression of these differentiation stages remains largely unknown. We identified a gain-of-function mutant altered phloem pole pericycle 1 Dominant (app1D), producing a truncated, nuclear-localized active form of NAC with Transmembrane Motif 1-like (NTL9). This mutation leads to ectopic expression of its downstream target CALLOSE SYNTHASE 8 (CalS8), thereby inducing callose accumulation, impeding SE differentiation, impairing phloem transport, and inhibiting root growth. The app1D phenotype could be reproduced by blocking the symplastic channels of cells within APP1 expression domain in wild-type (WT) roots. The WT APP1 is primarily membrane-tethered and dormant in the root meristem cells but entries into the nucleus in several cells in PPP near the unloading region, and this import is inhibited by blocking the symplastic intercellular transport in differentiating SE. Our results suggest a potential maintenance mechanism involving an APP1-CalS8 module, which induces CalS8 expression and modulates symplastic communication, and the proper activation of this module is crucial for the successful differentiation of SE in the Arabidopsis root.

Effects of Comonomers on the Performance of Stable Phosphonate-Based Gel Terpolymer Electrolytes for Sodium-Ion Batteries with Ultralong Cycling Stability.

Gel polymer electrolyte (GPE) is one of the most promising alternatives to solve the bottlenecks of nonaqueous liquid electrolytes such as decomposition, safety hazards, and growth of dendrites. In this work, three novel methyl phosphonate-based crosslinking gel terpolymer electrolytes with different comonomers are designed and prepared by in situ radical polymerization. The gel polymer electrolytes have excellent thermal stability, wide electrochemical windows (≥4.9 V), and high ionic conductivities (±3 mS cm-1), and may be used as less-flammable electrolytes for sodium-ion batteries. 31P NMR spectra, Arrhenius plot, and density functional theory (DFT) calculations confirm that multifunctional phosphonate structural units promote the dissociation of NaClO4 and help to transport the sodium ions freely in the polymer framework. X-ray photoelectron spectroscopy (XPS) results show that the gel polymer electrolytes have the capability of inhibiting liquid electrolyte decomposition and the formation of the stable solid electrolyte interphase (SEI) film. The Na3V2(PO4)3/GPE/Na cells exhibit better ultralong cycling stability and enhanced temperature performance than those of liquid cells. Strikingly, GPE1 has the best comprehensive electrochemical performance, especially the rate performance and long-term cycling stability with a capacity retention ratio of 82.6% after 3500 cycles, which indicates that different comonomers have obvious effects on the performance. Therefore, the full cell of SnS2/GPE1/Na3V2(PO4)3 is evaluated and delivers good cycling stability of 500 cycles, holding a great prospect for the design and production of phosphorus-containing electrolytes for safer sodium-ion batteries.

Influence of counter ions on structure, morphology, thermal stability of lanthanide complexes containing dipicolinic acid ligand.

Two kinds of lanthanide coordination polymers formed by dipicolinic acid with lanthanide ions were synthesized by varying the counter ions. And their crystal structures, morphology and thermal stabilities were measured and compared. X-ray single-crystal diffraction analysis reveals that Na3[Ln(DPA)3] (Ln = Tb or Eu) stretches to a rigid network by means of bridging Na+ ion. Moreover, Na3[Ln(DPA)]3 exhibits good thermal stability and luminescent properties, and its optical properties can be remained even after heating at 200 °C more than 3 days. However, when Na+ in Na3[Ln(DPA)3] was replaced with NH4+, i.e., (NH4)3[Ln(DPA)3] with a supramolecular structure based on π-π stacking and other weak interactions, shows relatively poor thermal stability which leads to deterioration of their luminescence properties after heating treatment. This result confirms that the rigid frame structure of Na3[Ln(DPA)]3 plays a crucial role in improving its thermal stability and keeping its highly luminescent quantum efficiency.

Using gastric juice lncRNA-ABHD11-AS1 as a novel type of biomarker in the screening of gastric cancer.

Long noncoding RNAs (lncRNAs) play vital roles in tumorigenesis. However, the diagnostic values of most lncRNAs are largely unknown. To investigate whether gastric juice lncRNA-ABHD11-AS1 can be a potential biomarker in the screening of gastric cancer, 173 tissue samples and 130 gastric juice from benign lesion, gastric dysplasia, gastric premalignant lesions, and gastric cancer were collected. ABHD11-AS1 levels were detected by reverse transcription-polymerase chain reaction. Then, the relationships between ABHD11-AS1 levels and clinicopathological factors of patients with gastric cancer were investigated. The results showed that ABHD11-AS1 levels in gastric cancer tissues were significantly higher than those in other tissues. Its levels in gastric juice from gastric cancer patients were not only significantly higher than those from cases of normal mucosa or minimal gastritis, atrophic gastritis, and gastric ulcers but also associated with gender, tumor size, tumor stage, Lauren type, and blood carcinoembryonic antigen (CEA) levels. More importantly, when using gastric juice ABHD11-AS1 as a marker, the positive detection rate of early gastric cancer patients was reached to 71.4 %. Thanks to the special origin of gastric juice, these results indicate that gastric juice ABHD11-AS1 may be a potential biomarker in the screening of gastric cancer.