RESUMO
In this study, a self-administered questionnaire was used to assess opinions about stem cell research and cord blood banking. Three attitudes were examined: willingness to accept cord blood banking, willingness to accept embryonic stem cell research, and religious belief system. A total of 90 Wayne State University students enrolled in the study in response to an invitation posted on a web page for the university. Sex distribution among study participants was 79 females and eight males; three declined to state their sex. Support for cord blood banking was high (> 70%) among students. Students over the age of 25 years of age were more (85%) positive than students 18 to 24 years old (57%). They prefered a public cord blood bank over a private cord blood bank. Atheist/agnostic or spiritual/not religious students (> 90%), Catholic students (78%) and Christian students (58%) support cord blood banking. Age, sex and religion seems influence the student's attitude towards stem cell research and cord blood banking.
Assuntos
Bancos de Sangue/ética , Transplante de Células-Tronco de Sangue do Cordão Umbilical/ética , Sangue Fetal/citologia , Opinião Pública , Pesquisa com Células-Tronco/ética , Estudantes/psicologia , Adolescente , Adulto , Fatores Etários , Atitude Frente a Saúde , Bancos de Sangue/estatística & dados numéricos , Transplante de Células-Tronco de Sangue do Cordão Umbilical/estatística & dados numéricos , Feminino , Sangue Fetal/fisiologia , Humanos , Masculino , Religião , Fatores Sexuais , Inquéritos e QuestionáriosRESUMO
Bone samples from a Middle Bronze Age (ca., 1600-1300 BC) site were prepared for histological analysis. Preliminary results suggested that components of bone marrow remained preserved. To verify these findings and optimize the sample preparation procedure, we conducted experiments varying the type of acid used to decalcify the bones for histology preparation, as well as the exposure time to the demineralizing agents and thickness of sections taken from the samples for slide preparation. Subsequent examination of the slides revealed the presence of well-preserved erythrocytes and other cellular structures consistent with bone marrow. Our results demonstrate that the traditional methods used to prepare bone samples for histology may be adjusted to improve the quality of the soft tissue architecture and cellular morphology for histological observation.
Assuntos
Antropologia Física/métodos , Osso e Ossos , Fósseis , Técnicas Histológicas/métodos , Células da Medula Óssea/citologia , Osso e Ossos/química , Osso e Ossos/citologia , Eritrócitos/citologia , HumanosRESUMO
Recent advances in the field of regenerative medicines manifested the unique properties of stem cells including the ability of self-renewal and differentiation to make them available for their replacement in tissue injury. Mesenchymal stem cells (MSCs) are important tools in treating immune disorders and in tissue repair due to their multipotency, immunosuppressive properties, and production of cytokines or growth factors. MSC-mediated therapy is a fast-growing field that has proven safe and effective in the treatment of various degenerative diseases and tissue injuries. Generation of induced pluripotent stem (iPS) cells holds a great promise for regenerative medicine and other aspects of clinical applications. The mechanisms governing multipotency in MSCs are not well understood. This review mainly throws light on the biology of MSCs, including their efficiency in treating several diseases and also the progress of the use of iPSC-derived MSC widely in the clinic.
