Effects of bed depths and the ratio of aerobic to anaerobic zone on the performance of horizontal subsurface flow macrophyte-assisted high-rate vermifilters treating synthetic brewery wastewater.

Effects of total vermibed depth, as well as the ratio of aerobic (the unsubmerged) to anaerobic (the submerged) zone on the performance of the horizontal subsurface flow macrophyte-assisted vermifilters (HSSF-MAVFs) treating synthetic brewery wastewater at a higher hydraulic loading rate (HLR), were investigated for the first time. Results showed that the HSSF-MAVF with a 50 cm total and 18 cm submerged vermibed depth yielded the optimum removal of the pollutants, ensuring a (91.2 ± 1.7)%, (81.8 ± 1.9)%, (67.4 ± 3.9)%, and (63.1 ± 2.3)% removal of chemical oxygen demand (COD), ammonium N (NH4 + -N), total N (TN), and organic N, respectively, whereas there was an increase of (142 ± 6.3)% in the effluent nitrate-N (NO3 - -N) than that in the influent. At the optimum condition, the effluent concentrations of all the pollutants including COD, NH4 + -N, NO3 - -N, TN, and organic N were well below the surface water discharge standards specified by the Central Pollution Control Board (CPCB), and thus, the effluent of the HSSF-MAVF could be safely discharged into the surface water bodies. PRACTITIONER POINTS: Total vermibed depth of HSSF-MAVFs was optimized for organic and nitrogen removal. HSSF-MAVFs were subjected to the higher HLR of synthetic brewery wastewater. Removal of COD and NH4 + -N was decreased with the increase in submerged bed depth. Removal of organic N and TN was increased with the increase in submerged bed depth. Total/unsubmerged bed depth had a positive impact on the organic and N removal.

Battery waste-derived magnetic Fe-Mn-Zn/C composites as an electro-Fenton-like catalyst for the degradation of sodium dodecyl sulfate surfactant.

A batch-scale electro-Fenton (EF) process was performed using graphite anode and waste battery-based Fe -Mn -Zn/C electrocatalyst coated on low-cost graphite felt cathode. The effectiveness of the EF's performance was evident with around 83.9 + 4.1% removal of 20 mg/L of sodium-dodecyl sulfate surfactant (SDS) at an optimum current density (CD) of 5.0 mA/cm2, Na2SO4 of 0.05 M, initial pH of 7.2, and electrolysis time of 180 min. Moreover, nearly 1.78-fold more removal of SDS was achieved in EF than in the electro-oxidation process operated without any catalyst. The operating cost of 0.35 $ of per m3 per order was needed to treat SDS wastewater. The remediation of SDS follows pseudo-first-order kinetics with a rate constant of 0.0095 min-1. Additionally, 90.3 + 2.1% of SDS and 57 + 2.6% of total organic carbon (TOC) removal was attained during 240 min of treatment time in secondary treated real wastewater; hence, additional 60 min of treatment time is required for effectively treating real wastewater than synthetic wastewater. Thus, EF is effective with battery waste-derived magnetic catalyst for treating wastewater containing SDS, which can lead to achieving sustainable environmental goals.

3D electro-Fenton augmented with iron-biochar particle electrodes derived from waste iron bottle caps and sugarcane bagasse for the remediation of sodium dodecyl sulphate.

The present work demonstrates a novel strategy of synthesizing iron-biochar (Fe@BCSB) composite made with the waste iron bottle cap and sugar cane bagasse for implementation in the three-dimensional electro-Fenton (3DEF) process. The catalytic ability of the Fe@BCSB composite was explored to remediate the sodium dodecyl sulphate (SDS) surfactant from wastewater at neutral pH. At the optimum operating condition of Fe@BCSB dose of 1.0 g L-1, current density of 4.66 mA cm-2, and Na2SO4 dose of 50 mM, nearly 92.7 ± 3.1% of 20 mg L-1 of SDS abatement was attained during 120 min of electrolysis time. Moreover, the Fe@BCSB showed significant recyclability up to six cycles. Besides, other organics were successfully treated with more than 85% abatement efficiency in the proposed Fe@BCSB-supported 3DEF process. The total operating cost obtained during SDS treatment was around 0.31 US$ m-3 of wastewater. The phytotoxicity test revealed the positive impact of the 3DEF-treated effluent on the germination of the Vigna radiata. The electron paramagnetic resonance conveyed •OH as the prevailing reactive species for the oxidation of SDS in the 3DEF process. Further, about 81.3 ± 3.8% of SDS and 53.7 ± 4.1% of mineralization efficacy were acquired from the real institutional sewage.

Spatiotemporal-based automated inundation mapping of Ramsar wetlands using Google Earth Engine.

Wetlands are one of the most critical components of an ecosystem, supporting many ecological niches and a rich diversity of flora and fauna. The ecological significance of these sites makes it imperative to study the changes in their inundation extent and propose necessary measures for their conservation. This study analyzes all 64 Ramsar sites in China based on their inundation patterns using Landsat imagery from 1991 to 2020. Annual composites were generated using the short-wave infrared thresholding technique from June to September to create inundation maps. The analysis was carried out on each Ramsar site individually to account for its typical behavior due to regional geographical and climatic conditions. The results of the inundation analysis for each site were subjected to the Mann-Kendall test to determine their trends. The analysis showed that 8 sites exhibited a significantly decreasing trend, while 14 sites displayed a significantly increasing trend. The accuracy of the analysis ranged from a minimum of 72.0% for Hubei Wang Lake to a maximum of 98.0% for Zhangye Heihe Wetland National Nature Reserve. The average overall accuracy of the sites was found to be 90.0%. The findings emphasize the necessity for conservation strategies and policies for Ramsar sites.

A critical review on the vermicomposting of organic wastes as a strategy in circular bioeconomy: mechanism, performance, and future perspectives.

To meet the current need for sustainable development, vermicomposting (VC), a natural, eco-friendly, and cost-effective technology, can be a wise selection for the bioconversion of organic wastes into value-added by-products. However, no one has tried to establish the VC technology as an economically sustainable technology by exploring its linkage to circular bioeconomy. Even, no researcher has made any effort to explore the usability of the earthworms (EWs) as a protein supplement while assessing the economic perspectives of VC technology. Very few studies are available on the greenhouse gas (GHG) emission potential of VC technology. Still, the contribution of VC technology towards the non-carbon waste management policy is not yet explored. In the current review, a genuine effort has been made to inspect the contribution of VC technology towards the circular bioeconomy, along with evaluating its capability to bioremediate the organic wastes generated from domestic, industrial, and agricultural premises. The potential of the EWs as a protein source has also been explored to strengthen the contribution of VC technology towards the circular bioeconomy. Moreover, the linkage of the VC technology to the non-carbon waste management policy has been comprehensively demonstrated by highlighting its carbon sequestration and GHG emission potentials during the treatment of organic wastes. It has been observed that the cost of food production was reduced by 60-70% by replacing chemical fertilizers with vermicompost. The implication of the vermicompost significantly lessened the harvesting period of the crops, thereby helping the farmers attain higher profits by cultivating more crops in a single calendar year on the same plot. Furthermore, the vermicompost could hold the soil moisture for a long time, lessening the water demand up to 30-40%, which, in turn, reduced the frequency of irrigation. Also, the replacement of the chemical fertilizers with vermicompost resulted in a 23% increment in the grapes' yield, engendering an extra profit of up to 110000 rupees/ha. In Nepal, vermicompost has been produced at a cost of 15.68 rupees/kg, whereas it has been sold to the local market at a rate of 25 rupees/kg as organic manure, ensuring a net profit of 9.32 rupees/kg of vermicompost. EWs embraced 63% crude protein, 5-21% carbohydrates, 6-11% fat, 1476 kJ/100 g of metabolizable energy, and a wide range of minerals and vitamins. EWs also contained 4.11, 2.04, 4.43, 2.83, 1.47, and 6.26 g/kg (on protein basis) of leucine, isoleucine, tryptophan, arginine, histidine, and phenylalanine, respectively, enhancing the acceptability of the EW meal (EWM) as the protein supplement. The inclusion of 3 and 5% EWM in the diet of broiler pullets resulted in a 12.6 and 22.5% increase in their feed conversion ratio (FCR), respectively after one month. Similarly, when a 100% fish meal was substituted by 50% EWM and 50% fish meal, the FCR and growth rate of Parachanna obscura were increased substantially. The VC of maize crop residues mixed with pig manure, cow dung, and biochar, in the presence of Eisenia fetida EWs, yielded only 0.003-0.081, 0-0.17, and 130.40-189.10 g CO2-eq.kg-1 emissions of CO2, CH4, and N2O, respectively. Similarly, the VC of tomato stems and cow dung ensured 2.28 and 5.76 g CO2-eq.kg-1 CO2 emissions of CH4 and N2O, respectively. Additionally, the application of vermicompost at a rate of 5 t/ha improved the soil organic carbon proportion and aggravated carbon sequestration. The land application of vermicompost improved micro-aggregation and cut down the tillage, reducing GHG emissions and triggering carbon sequestration. The significant findings of the current review suggest that VC technology potentially contributes to the concept of circular bioeconomy, substantially negotiates potential GHG emissions, and complies with the non-carbon waste management policy, reinforcing its acceptability as an economically sound and environmentally benevolent organic waste bioremediation alternative.

Using innovative copper-loaded activated alumina (Cu/AA) as the catalyst for catalytic wet peroxidation (CWPO) of catechol.

In this study, copper-loaded activated alumina (Cu/AA) was synthesized and used for the CWPO of catechol (a representative refractory organic pollutant). Various characterization techniques were deployed to characterize the catalysts, e.g., activated alumina (AA), as well as pristine and spent 1% Cu/AA. The innovative 1% Cu/AA catalyst exhibited good thermal stability up to 1173 K with a marginal weight loss of 13%. The Cu species were well dispersed on the activated alumina framework with no significant cluster formation. Typically, the average copper particle size of 5 nm was dispersed on the AA framework. Catechol removal was observed to be 92% with 87% mineralization at optimized conditions (initial catechol concentration = 200 mg/L, catalyst dose of 1% Cu/AA = 2 g/L; temperature = 323 K; pH = 6; and H2O2/catechol stoichiometric ratio = 0.5). The mineralization of catechol was analyzed using mass spectroscopy, with the associated mechanism has been elucidated. Results of this study indicated that synthesized catalyst has phenomenal advantages in terms of simple separation and high removal efficiency of catechol, suggesting the feasibility of employing Cu/AA as the effective catalyst for the CWPO of catechol.

Vermifiltration: Strategies and techniques to enhance the organic and nutrient removal performance from wastewater.

The vermifiltration (VF) technology has gained significant attention as a green alternative for remediating domestic and industrial wastewater over the last few decades. Of late, the implementation of various modifications to the orthodox VF technology, including tweaks in the design and operation of the vermifilters, has been portrayed in the available literature. However, owing to the scatteredness of the available information, the knowledge regarding the execution of the modified vermifilters is still inadequate. Hence, an effort has been made to comprehensively overview the innovative strategies and techniques adopted to improve the organic and nutrient removal potential of the VFs from wastewater. In addition, future perspectives have been recognized to design more efficient and sustainable VFs. This review explores more of such novel tactics to improve the performance of the VF technology regarding organic and nutrient removal from wastewater. PRACTITIONER POINTS: Innovative strategies and techniques implemented to VF technology were comprehensively overviewed. Design modification and advantages of each innovation were highlighted. The pollutant removal performance of every modification was emphasized. Modified vermifilters were better than the conventional vermifilters in terms of organic and nutrient removal from the wastewater.

Comparison of GHG emissions from open field burning and anaerobic digestion of rice straw.

Rice straw (RS) has been burned in open fields in many countries, which is a growing environmental concern. Anaerobic digestion (AD) of RS is one of the sustainable alternatives to open burning of RS for converting the biomass into methane, carbon dioxide, and digestate for use as agricultural manure. The present study evaluates the RS generation, use patterns, and greenhouse gas emissions from open field burning and the AD of RS. Results indicate the AD of RS offers greater environmental benefits than that by RS open field burning. As a result of the open burning of RS in India emitted 592032.52 kg of CO2 equivalents/kg per year, whereas AD of RS contributes 122.86 kg of CO2 equivalents/kg per year. If RS were burned in an open field, its Global Warming Potential would be 493,873 times greater than that of AD. By minimizing GHG emissions and creating sustainable energy from waste, sustainable development, and circular economies are achieved.

Toxicity determination, pollution source delineation, and microbial diversity evaluation of PAHs-contaminated sediments for an urban river.

The Feng-Sang River is a metropolitan river in Kaohsiung City, Taiwan. In this study, Feng-Sang River sediments were analyzed to investigate the distributions and sources of polycyclic aromatic hydrocarbons (PAHs). The Sediment Quality Guidelines (SQGs), potentially carcinogenic PAHs (TEQcarc ), and toxic equivalence quotient (TEQ) were applied to evaluate influences of PAHs on ecosystems and microbial diversities. Results indicate that PAHs concentrations varied between seasons and locations. The concentrations of ∑16 PAHs ranged from 73.6 to 603.8 ng/kg in dry seasons and from 2.3 to 199.3 ng/kg in wet seasons. This could be because of the flushing effect during wet seasons, which caused the movement and dilution of the PAH-contaminated sediments. Diagnostic ratio analysis infers that high PAHs levels were generated by combustion processes and vehicle traffic, and results from multivariate descriptive statistical analysis also demonstrate that the vehicular traffic pollution could be the major emission source of PAHs contamination. Comparisons of PAHs with SQGs indicate that PAHs concentrations in sediment were below the effects range low (ERL) values, and thus, the immediate threat to organisms might not be significant. The diagnostic ratio analyses are effective methods for PAH source appointment. The metagenomic assay results imply that sediments contained essential microbial species with eminent diversity. The detected PAH-degrading bacteria (Desulfatiglans, Dechloromonas, Sphingomonas, Methylobacterium, Rhodobacter, Clostridium, and Exiguobacterium) played a key role in PAHs biotransformation, and Dechloromonas and Rhodobacter had a higher relative abundance. Results of microbial diversity analyses indicate that the contaminated environment induced the changes of governing microbial groups in sediments. PRACTITIONER POINTS: Diagnostic ratio analyses are effective methods for PAHs source appointment. Microbial composition in sediments are highly affected by anthropogenic pollution. Combustion and vehicle traffic contribute to urban river sediments pollution by PAHs. Dechloromonas and Rhodobacter are dominant PAHs-degrading bacteria in sediments.

Dark fermentation: Production and utilization of volatile fatty acid from different wastes- A review.

Volatile fatty acids (VFAs) are the building blocks of the chemical industry, and they are the primary contributors to the planet's organic carbon cycle. VFA production from fossil fuels (mostly petroleum) is unsustainable, pollutes the environment, and generates greenhouse gases. As a result of these issues, there is a pressing need to develop alternate sources for the long-term generation of VFAs via anaerobic digestion. The accessible feedstocks for its sustainable production, as well as the influencing parameters, are discussed in this review. The use of VFAs as a raw material to make a variety of consumer products is reviewed in order to find a solution. It also bridges the gap between traditional and advanced VFA production and utilization methods from a variety of solid and liquid waste sources for economical stability.

Greenhouse gas emissions in sludge ultrasonication followed by anaerobic digestion processes.

This paper presents the greenhouse gas (GHG) emissions during ultrasonication of sludge and anaerobic digestion (AD) of the ultrasonicated sludge using mass-energy balance. Computation of the net energy (energy recovered - energy input) revealed that high solids concentration with low sonication specific energy provides positive net energy. Moreover, the GHG emissions can be minimised at low sonication specific energy input and high solids concentration compared to that of the control without sonication. Increase in temperature of sludge during sonication will reduce the energy input required for raising the sludge temperature to AD temperature and thus decreases the GHG emissions. With energy recovery from the methane produced in AD, the total GHG emissions can further be reduced, lower than that without energy recovery from methane.

Nutrient removal from domestic wastewater: A comprehensive review on conventional and advanced technologies.

Nitrogen and phosphorous are indispensable for growth and vitality of living beings, hence termed as nutrients. However, discharge of nutrient rich waste streams to aquatic ecosystems results in eutrophication. Therefore, nutrient removal from wastewater is crucial to meet the strict nutrient discharge standards. Similarly, nutrient recovery from waste streams is vital for the realization of a circular economy by avoiding the depletion of finite resources. This manuscript presents analysis of existing information on different conventional as well as advanced treatment technologies that are commonly practiced for the removal of nutrient from domestic wastewater. First, the information pertaining to the biological nutrient removal technologies are discussed. Second, onsite passive nutrient removal technologies are reviewed comprehensively. Third, advanced nutrient removal technologies are summarized briefly. The mechanisms, advantages, and disadvantages of these technologies along with their efficiencies and limitations are discussed. An integrated approach for simultaneous nutrient removal and recovery is recommended. The fifth section of the review highlights bottlenecks and potential solutions for successful implementation of the nutrient removal technologies. It is anticipated that the review will offer an instructive overview of the progress in nutrient removal and recovery technologies and will illustrate necessity of further investigations for development of efficient nutrient removal and recovery processes.

Critical insights into psychrophilic anaerobic digestion: Novel strategies for improving biogas production.

Anaerobic digestion (AD) under psychrophilic temperature has only recently garnered deserved attention. In major parts of Europe, USA, Canada and Australia, climatic conditions are more suited for psychrophilic (<20 ℃) rather than mesophilic (35 - 37 ℃) and thermophilic (55 - 60 ℃) AD. Low temperature has adverse effects on important cellular processes which may render the cell biology inactive. Moreover, cold climate can also alter the physical and chemical properties of wastewater, thereby reducing the availability of substrate to microbes. Hence, the use of low temperature acclimated microbial biomass could overcome thermodynamic constraints and carry out flexible structural and conformational changes to proteins, membrane lipid composition, expression of cold-adapted enzymes through genotypic and phenotypic variations. Reduction in organic loading rate is beneficial to methane production under low temperatures. Moreover, modification in the design of existing reactors and the use of hybrid reactors have already demonstrated improved methane generation in the lab-scale. This review also discusses some novel strategies such as direct interspecies electron transfer (DIET), co-digestion of substrate, bioaugmentation, and bioelectrochemical system assisted AD which present promising prospects. While DIET can facilitate syntrophic electron exchange in diverse microbes, the addition of organic-rich co-substrate can help in maintaining suitable C/N ratio in the anaerobic digester which subsequently can enhance methane generation. Bioaugmentation with psychrophilic strains could reduce start-up time and ensure daily stable performance for wastewater treatment facilities at low temperatures. In addition to the technical discussion, the economic assessment and future outlook on psychrophilic AD are also highlighted.

Application of zeolitic imidazolate framework for hexavalent chromium removal: A feasibility and mechanism study.

The mechanisms and effectiveness of using zeolitic imidazolate framework (ZIF-8) [a sub-family of metal-organic framework (MOF)] particles on hexavalent chromium [Cr(VI)] removal were evaluated. The ultrasonic mixing method was applied for the preparation of ZIF-8, and chemicals used for ZIF-8 synthesis included ammonium hydroxide, zinc nitrate hexahydrate, and 2-methylimidazole. ZIF-8 particle had a clear rhombic dodecahedron morphology shape and a strong peak intensity with high crystallinity. The adsorption capacity (AC) of ZIF-8 was 30.3 mg of Cr(VI)/g of ZIF-8 [Cr(VI) = 50 mg/L]. The AC of Cr(VI) raised to 34.3 mg/g under acidic conditions (pH = 5), and the AC dropped to below 13.7 mg/g with a pH range from 7 to 11. It could be because of the competitive effects between CrO4 2- and hydroxide ions for adsorption locations of ZIF-8. Cr(VI) removal relied on the amount of Cr(VI) adsorbed on the particles of ZIF-8, and the mechanisms of Cr(VI) adsorption by ZIF-8 included chemical/physical processes and the rate-limiting step was the chemical adsorption. A fraction of sorbed Cr(VI) was reduced to Cr(III), and thus, ZIF-8 could serve as a reducing agent during Cr(VI) reduction. Cr(VI) was removed effectively from the water phase by ZIF-8 via adsorption and reduction mechanisms. PRACTITIONER POINTS: ZIF-8 particles had an adsorption capacity of 30.33 mg of Cr(VI)/g of ZIF-8. Cr(VI) sorption by ZIF-8 has chemical (rate-limiting step) and physical processes. ZIF-8 can serve as a reducing agent for Cr(VI) reduction. Cr(VI) can be removed by ZIF-8 via the adsorption and reduction mechanisms.

Treatment technologies for emerging contaminants in wastewater treatment plants: A review.

The "emerging contaminants" (ECs) are predominantly unregulated anthropogenic chemicals that occur in air, soil, water, food, and human/animal tissues in trace concentrations. The ECs are persistent in the environment, capable of perturbing the physiology of target receptors and, therefore, are regarded as contaminants of emerging environmental concerns in recent years. The prominent classes of ECs include pharmaceuticals and personal care products (PCPs), surfactants, plasticizers, pesticides, fire retardants, and nanomaterials. Some of the ECs with harmful effects on endocrine systems have been recognized as endocrine disrupting chemicals (EDCs). Since the 1990s intensive research has been done covering environmental occurrence, fate, ecological effects, and treatment technologies of ECs. However, a comprehensive summary of the EC removal techniques, particularly in wastewater treatment plants (WWTPs) are limited. Though the WWTPs are inefficient when it comes to ECs removal, they act as primary barriers against the spread of ECs. Therefore, this paper reviews the treatment technologies currently engaged for ECs removal in WWTPs for further possible upgrades of the existing designs. Results of this review indicate that the fate and distribution of ECs can be approximately estimated based on physicochemical properties like octanol-water partitioning coefficient (e.g., log KOW > 4, maximum sorption potential) and solid-water distribution coefficient [e.g., Kd < 300-500 L/kg MLSS (mixed liquor suspended solids), insignificant sorption into sludge]. Biodegradation potential of ECs can be predicted from biodegradation constant values (e.g., Kbio < 0.01 = low biodegradation and >10 = high biodegradation). In WWTPs, the EC removal efficiency varies in the range of 20-50%, 30-70%, and >90% during the primary, secondary, and tertiary treatment steps, respectively. Tertiary treatment technologies are considered as the most suitable alternatives for ECs treatment, but complete ECs removal is yet to be achieved. Further advancements in the treatment technologies will unquestionably be necessary in the future.

Ciprofloxacin removal via sequential electro-oxidation and enzymatic oxidation.

The combination of electro-oxidation and enzymatic oxidation was tested to evaluate the potency of this system to remove ciprofloxacin (CIP), a fluoroquinolone antibiotic, from water. For the electro-oxidation boron-doped diamond (BDD) and mixed metal oxides anodes were tested, at three current densities (4.42, 17.7 and 35.4 A/cm2). BDD anode at 35.4 A/cm2 exhibited the highest removal efficiency in the shortest time (>90 % removal in 6 min). For the enzymatic oxidation, laccase from Trametes versicolor was chosen. Laccase alone was not able to remove CIP; hence the influence of redox mediators was investigated. The addition of syringaldehyde (SA) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) resulted in enhanced CIP transformation. About 48.9±4.0 % of CIP remained after 4 h of treatment when SA-mediated laccase was applied and 87.8±6.6 % in the case of ABTS-mediated laccase. The coupling of enzymatic oxidation followed by electro-oxidation led to 73 % removal of the antibiotic. Additionally, the antimicrobial activity increased up to its original efficiency after the treatment. The combination of electro-oxidation followed by enzymatic oxidation led to 97-99 % removal of CIP. There was no antimicrobial activity of the solution after the treatment. The tests with wastewater confirmed the efficacy of the system to remove CIP from the complex matrix.

The potential utilization of slag generated from iron- and steelmaking industries: a review.

Along with iron and steel production, large amount of slag is generated. Proper management on the iron- and steelmaking slag is highly demanded due to the high cost of direct disposal of the slag to landfill, which is the most adopted management approach. In this article, the potential application of iron- and steelmaking slag has been reviewed, which included the slag utilization in construction as cement and sand, in water, soil, and gas treatment, as well as in value material recovery. In addition, the challenge and required effort to be made in iron- and steelmaking slag management have been discussed.

Physical and biological removal of Microcystin-LR and other water contaminants in a biofilter using Manganese Dioxide coated sand and Graphene sand composites.

Sand as a filter media is often challenged by the presence of organics in the form of natural organic matter, metal ions, and various micropollutants in the source water. It is mainly due to the presence of limited active adsorption sites and low surface area that governs an ineffective adsorption potential of the sand material. Herein, graphitized sand was synthesized to tackle the above limitations using two sugar solution sources: a) brewery effluent (as a low-cost solution) (GS1) and; b) sucrose solution (GS2). GS1 showed 68%, 60%, and 99% higher maximum adsorption constant (qmax) for divalent metal ions: iron, copper, and manganese, respectively as compared to raw sand (RS). Coating of MnO2 over the graphitized sand (GSMs: GS1M and GS2M) further helped in Microcystin-LR (MC-LR) removal (3%-9%) when inoculated with MC-LR-degraders, but was not as effective in removing metals, organic carbon and nitrogen when compared to just graphitized sand (GS1 or GS2). Inoculating GS and GSMs (for both sugar sources) not only helped in higher MC-LR removal (10%-15% more) but also enhanced the removal of other water contaminants including metals, organic nitrogen, and carbon. GS1 showed 20% and 50% more MC-LR removal than the sand material when tested at a low and high initial concentration of MC-LR (5 µg/L and 50 µg/L). The highest breakthrough period was obtained for GS1 filter using 1 mg/L Rhodamine-B dye, which was 12 times (48 min) more than the raw sand filter and almost 2.5 times (second best, 21 min) than GS1M. After three cycles of regeneration and reuse of GS1 filter, a decrease of just 14% in saturation adsorption capacity indicated its high reusability aspects.