[The effect of separate and consecutive administration of cytochrome P-450 inducers to rats on the localization of various isoforms of the enzyme in liver lobe cells]. / Vliianie razdel'nogo i posledovatel'nogo vvedeniia krysam induktorov tsitokhroma P-450 na lokalizatsiiu razlichnykh izoform fermenta v kletkakh pechenochnoi dol'ki.

By means of immunohistochemical methods a study of the liver intralobular localization of cytochromes P-450b(+e) and P-450c(+d) has been carried out after separate and consecutive treatment of rats with phenobarbital (PB) and 3-methylcholanthrene (MC). PB-treatment leads to localization of P-450b in the centrilobular region, whereas homogeneous distribution of P-450c in the lobule is observed after MC-treatment. The consecutive treatment with PB and MC is accompanied by the localization of P-450c only in cells of the periportal region of the lobule. PB-treatment of rats after preliminary MC-injection also results in the periportal localization of P-450c, and a small quantity of P-450b is localized in hepatocytes of the centrilobular region. Hence, the consecutive treatment with inducers of different molecular forms of cytochrome P-450 is accompanied by the redistribution of these isoenzymes in parenchymatous cells of the liver lobule. That is confirmed also by biochemical testing and immunochemical analysis of the microsomal fraction of hepatocytes.