RESUMO
The effect of therapeutic doses of a hormonal cytostatic cortifen and corticosterone on supramolecular DNA structures of mice thymocyte nucleoid was studied. Capillary elastoviscometry showed that in vivo damage to supramolecular DNA complex structure there was a difference already two hours after injection of these agents. After 24 hours the damaging effects on nucleoid were markedly higher. The role of the hormone and alkylating group of cortifen in the cytotoxic action of antitumor agents is discussed.
Assuntos
Núcleo Celular/efeitos dos fármacos , Corticosterona/análogos & derivados , Corticosterona/farmacologia , Compostos de Mostarda Nitrogenada/farmacologia , Timo/efeitos dos fármacos , Animais , Células Cultivadas , DNA Super-Helicoidal/efeitos dos fármacos , Relação Dose-Resposta a Droga , Combinação de Medicamentos/farmacologia , Elasticidade , Etídio/farmacologia , Camundongos , Relação Estrutura-Atividade , Fatores de Tempo , ViscosidadeRESUMO
The damaging effects on the structure of a supramolecular DNA complex of sarcoma-37 cells in mice following the use of the therapeutic doses of khanerol were found. Capillary elastoviscosimetry and nucleoid sedimentation procedure showed that the damage of the supramolecular DNA complex structure becomes apparent already after 4 hours, and increases by 24 hour. In studies of the binding of khanerol with DNA by Cd and Tmelt. the procedure showed direct interaction of khanerol with DNA. The role of conformational changes of the supramolecular DNA complex in the cytotoxic action of antitumour agents is discussed.