Simultaneous Determination of Puerarin and Baicalin in Xiao’ er Shuangjie Zhixie Granules by HPLC / 中国药师

Objective:To establish a method for the determination of puerarin and balcalin in Xiao’ er Shuangjie Zhixie granules by HPLC. Methods:An Agilent Zorbax SB-C18 column (250 mm × 4. 6 mm,5 μm) was used. The mobile phase A was 0. 1% phos-phoric acid solution and the mobile phase B was methanol with gradient elution. The detection wavelength was 265 nm,the flow rate was 1. 0 ml·min-1 ,the column temperature was 30℃ and the sample size was 20 μl. Results: The linear range of pueratin was 0. 121-1. 942 μg (r=0. 999 5)and that of balcalin was 0. 230-3. 686 μg(r=0. 999 5). The average recovery was 98. 10% and 98. 20%with RSD of 1. 34% and 1. 11%(n=6), respectively. Conclusion:The method is convenient and accurate, which can be used in the de-termination of pueraln and balcalin in Xiao’ er Shuangjie Zhixie granules.

Analysis of content changes of volatile oil and beta-elemene in oil in Sarcandrae / 中国中药杂志

<p><b>OBJECTIVE</b>To reveal the factors that affect the contents of volatile oil and beta-elemene in oil in Sarcandrae and to provide scientific basis for Sarcandrae's reasonable exploitation and quality assessment.</p><p><b>METHOD</b>A GC method was established and the contents of volatile oil and beta-elemene were determined in Sarcandrae from different length of time to grow, different medicament portions, different collection periods and different length of time to dry.</p><p><b>RESULT</b>The length of time to grow had no significant effect and the collection periods had effect on the volatile oil yield and content of beta-elemene. The volatile oil yield and content of beta-elemene were the highest in Sarcandrae harvested in December. The yield of volatile oil in different medicament portions descended in an order of roots, leaves and stems. The content of beta-elemene was the highest in leaves and the lowest in stems. With the increasing of the length of time to dry, the volatile oil yield and content of beta-elemene decreased calculated on an anhydrous basis.</p><p><b>CONCLUSION</b>The established method is simple, accurate and repeatable. It can be used for the quality control of beta-elemene in Sarcandrae. The study provides a valuable basis for the quality assessment, the development and utilization of Sarcandrae.</p>

Preparation of submicron emulsion of fresh Zhongjiefeng volatile oil / 中国中药杂志

<p><b>OBJECTIVE</b>To prepare the submicron emulsion of fresh Zhongjiefeng volatile oil.</p><p><b>METHOD</b>The Zhongjiefeng volatile oil submicron emulsion was obtained after passing the elementary emulsion through a high pressure homogenizer. The physical and chemical stability of the emulsion was evaluated with the stability parameter of centrifugation, appearance of emulsion and the pH. The formulation and processing factors were optimized by single factor reviewing and orthogonal experimental design.</p><p><b>RESULT</b>By controlling various processing factors and optimizing formulation, the stable submicron emulsion of Zhongjiefeng volatile oil was prepared. Its mean particle diameter was 164-169 nm with PDI 0.084-0.107 and Zeta electric potential was -40 mV.</p><p><b>CONCLUSION</b>The formulation and preparation technique of the emulsion is reasonable.</p>

Value of anti-survivin in early diagnosis of malignant tumors / 第三军医大学学报

Objective To establish a soluble expression of recombinant survivin in E. coli, and evaluate the role of anti-survivin in tumor diagnosis. Methods The recombinant survivin was screened by ampicillin resistance, identified by PCR and double digestion of endonucleases. The sequenced DNA of survivin was analyzed by BLAST. The survivin/Trx fusion protein expressed in E.coli BL21 (DE3) was induced with IPTG, identified by Western blot and purified with Ni-NTA agarose respectively. The anti-survivin antibodies in serum were detected with an indirect ELISA. By the methods above, 144 serum samples from cancer patients and 300 serum samples from normal subjects were analyzed. Meanwhile, the joint detection of anti-survivin, AFP, CEA, CA199, CA125 in blood sera from cancer patients was detected. Results After PCR and double digestion with endonucleases, the survivin gene was inserted into the prokaryotic expression vector PET 32a(+). After DNA sequencing, the constructed expression plasmid was transformed into competent cells E.coli BL21(DE3). Western blot identified that the recombinant survivin/Trx fusion protein was specific against survivin antibody. The purity of the protein was over 96% after purified by Ni-NTA agarose. Anti-survivin was expressed in the sera of different cancer patients, but the positive rate varied. Conclusion Prokaryotic expression plasmid PET 32a(+)/survivin was successfully constructed and highly purified survivin/Trx fusion protein was obtained. The detection results of anti-survivin, AFP, CEA, CA199, CA125 in blood serum show in the diagnosis of tumors, the joint detection can overcome the shortfall of each gene and enhance the diagnostic rate.

Exquisite Lectures,Mutual Participation,Personal Experience,and Sublimation——Exploration and Introduction of a New Model in Teaching Medical Ethics / 中国医学伦理学

This article introduces the development and experience of the educational reforms in the teaching of medical ethics at Sun Yat-Sen University,concerning the "three-in-one" model,constituting "classroom instruction,practical experience,and independent research".Practical experience is considered an effective way of implementing and consolidating theories and practices,just as knowledge in professional ethics are exercised in coherence.It is an important step for medical students to have early contacts with society,enhance social responsibility,increase understanding and practices of medical ethics and developing practical and creative abilities.

Studies on TLC identification of root bark of Morus alba / 中草药

Object To study specific TLC identification of the root bark of Morus alba L. (RBMA)Methods The ethanol extracts of RBMA and other confusable species were subjected to TLC in different development system. The TLC plats were examined under shortwavelength UV light or colored by FeCl 3 solution.Results A mixture of chloroform and methanol (5∶1) is used for development and ferric chloride solution is used to color, a specific purple spot of the certified RBMA can be found.Conclusion The specific spot showed with TLC can be regarded as the basis of identification of RMBA and it was separated and identified as sanggenon C.

GC fingerprint of the constituents in the volatile oil of Eucalyptus globulus Labill / 中成药

AIM:To establish a method for GC fingerprint determination of the chemical constituents in the volatile oil of Eucalyptus globulus Labill.in Yunnan Province,China. METHODS: GC was used to optimize the fingerprint determination method,and to identify the main peaks in the GC fingerprint. RESULTS: 16 mutual peaks were gained on the base of 10 batches of samples. CONCLUSION: Stable and reproducible GC fingerprint is helpful to further analyze the volatile oil constituent of Eucalyptus globulus Labill.