RESUMO
Changes in the content and composition of fatty acids in phospholipids were determined to estimate the effect of propranolol on the growth of unicellular green algae Chlorella vulgaris cultivated synchronously. The general biological activity of the cells and the division coefficient (DC) as well as the cell synchronisation were disturbed by propranolol in a concentration dependent manner. Also, the effect of propranolol on the fatty acids content of Chlorella vulgaris phospholipids was proportional to its concentration.
Assuntos
Antiarrítmicos/farmacologia , Chlorella/efeitos dos fármacos , Ácidos Graxos/análise , Fosfolipídeos/análise , Propranolol/farmacologia , Ciclo Celular/efeitos dos fármacos , Ciclo Celular/fisiologia , Chlorella/química , Cromatografia Gasosa , Espectrometria de Massas , Lipídeos de Membrana/análiseRESUMO
The effect of dopamine-melanin (DA-melanin), synthetic model of neuromelanin, on the stability of 13-hydroperoxyoctadecadienoic acid (13-HPODE) has been investigated using a reverse-phase high-performance liquid chromatography (HPLC) with UV detection. It was found that DA-melanin effectively accelerated the decomposition of 13-HPODE, both in the absence and in the presence of ferrous ions. The disappearance of 13-HPODE was accompanied by the formation of 13-hydroxyoctadecadienoic acid (13-HODE). The results obtained indicate that DA-melanin is able to reduce linoleic acid hydroperoxide to its stable hydroxyl derivative. The fatty acid hydroperoxide-reducing ability of DA-melanin appears to play an important role in the antioxidative activity of neuromelanin in the process of lipid peroxidation.
