RESUMO
La dopamina es sintetizada por la tirosina hidroxilasa y es considerada como una catecolamina mayor en la retina de los vertebrados, incluyendo el pez cebra. Sin embargo, se conoce poco sobre la función del receptor de dopamina D2 (DRD2) en la fisiología retiniana. Por lo tanto, para dilucidar el papel del DRD2 en el desarrollo y la función de los ojos en el pez cebra, los peces fueron expuestos a la flufenazina, quinpirol, o la combinación de ambos, y luego se evaluó el tamaño del ojo, el diámetro del nervio óptico (ONd) y la adaptación visual al fondo. Los resultados mostraron que la flufenazina (flufenazina, antagonista DRD2) disminuyó el tamaño del ojo y el diámetro del nervio óptico seguido de una interrupción de la función visual. La adición de quinpirol (quinpirol, agonista DRD2) invirtió los efectos causados por flufenazina, lo que implica que DRD2 es necesario para el desarrollo y la función normal del ojo en el pez cebra. Considerando el papel de las neuronas dopaminérgicas en el desarrollo y la función de la retina, la disfunción de las vías de señalización de las neuronas dopaminérgicas en la retina puede causar anormalidades visuales, particularmente en la participación de la dopamina en la regulación de la respuesta de la luz
Dopamine is synthesized by tyrosine hydroxylase and is considered as a major catecholamine in the vertebrate retina, including zebrafish. However, little is known about the role of dopamine D2 receptor (DRD2) in retinal physiology. Therefore, to elucidate the role of DRD2 in the eye development and function in zebrafish, fish were exposed to fluphenazine, quinpirole, or combination of both. Subsequently, the eye size, optic nerve diameter (ONd), and visual background adaptation were evaluated. The results showed that fluphenazine (fluphenazine, DRD2 antagonist) decreased eye size and optic nerve diameter followed by disruption of visual function. The addition of Quinpirole (quinpirole, DRD2 agonist) reversed the effects caused by fluphenazine, implying that DRD2 is necessary for normal eye development and function in zebrafish. Considering the role of dopaminergic neurons in retinal development and function, dysfunction of dopaminergic neuron signaling pathways in the retina may cause visual abnormalities, particularly in the involvement of dopamine in regulating light response
Assuntos
Animais , Antagonistas dos Receptores de Dopamina D2/farmacologia , Olho/embriologia , Fenômenos Fisiológicos Oculares , Receptores de Dopamina D2/fisiologia , Olho/anatomia & histologia , Olho/crescimento & desenvolvimento , Flufenazina/farmacologia , Imuno-Histoquímica , Tamanho do Órgão , Quimpirol/farmacologiaRESUMO
Dopamine is synthesized by tyrosine hydroxylase and is considered as a major catecholamine in the vertebrate retina, including zebrafish. However, little is known about the role of dopamine D2 receptor (DRD2) in retinal physiology. Therefore, to elucidate the role of DRD2 in the eye development and function in zebrafish, fish were exposed to fluphenazine, quinpirole, or combination of both. Subsequently, the eye size, optic nerve diameter (ONd), and visual background adaptation were evaluated. The results showed that fluphenazine (fluphenazine, DRD2 antagonist) decreased eye size and optic nerve diameter followed by disruption of visual function. The addition of Quinpirole (quinpirole, DRD2 agonist) reversed the effects caused by fluphenazine, implying that DRD2 is necessary for normal eye development and function in zebrafish. Considering the role of dopaminergic neurons in retinal development and function, dysfunction of dopaminergic neuron signaling pathways in the retina may cause visual abnormalities, particularly in the involvement of dopamine in regulating light response.
