[Features of expression of GLUT-1 and Ki-67 proteins in various components of mixed variants of ameloblastoma]. / Osobennosti ekspressii belkov GLUT-1 i Ki-67 v razlichnykh komponentakh smeshannykh variantov ameloblastomy.

THE AIM OF THE STUDY: The aim was to study the metabolic and proliferative activity of the components of ameloblastoma of mixed histological structure. To assess the impact of individual components of mixed variants of ameloblastoma on treatment results and the risk of relapse. MATERIAL AND METHODS: The study included 21 histological specimens of mixed ameloblastoma. To study proliferative and metabolic activity, histological preparations were immunohistochemically stained. To assess the proliferation of tumor components, histological preparations were stained for the presence of antigens to Ki-67, and the level of metabolic activity was assessed by the expression level of the glucose transporter GLUT-1. Statistical analysis was performed using the Mann-Whitney test, statistical significance was determined using the Chi-square test, and correlation analysis was performed using Spearman's. RESULTS: Among the samples of mixed ameloblastoma included in the study, a non-uniform distribution of proliferation and intensity of metabolic activity among the components was obtained. So, among all the components, the plexiform and basal cell variants are distinguished by the greatest proliferative activity. The metabolic activity of these components of mixed ameloblastoma is also increased. CONCLUSIONS: The data obtained allow us to conclude that it is necessary to take into account plexiform and basal cell components of mixed ameloblastoma, as this can affect the effectiveness of treatment and the risk of relapse.

[Stafne defect as a rare anomaly of the lower jaw growth]. / Defekt Stafne ­ redkaya anomaliya razvitiya nizhnei chelyusti.

Defects in mandible growth are of undoubted interest for practical healthcare. During the diagnosis, for a more accurate diagnosis and differential diagnosis, it is necessary to understand the criteria for the norm and pathology of diseases of the jaw bones. Often in the body of the mandible at the level of the lower molars, slightly below the maxillofacial line, defects are detected in the form of a depression of the cortical layer towards the unchanged buccal cortical plate. These defects, being the clinical norm, need to be differentiated with many tumor diseases of the maxillofacial region. The literature sources indicate that the cause of these defects is the pressure of the capsule of the submandibular salivary gland in the area of the fossa on the lower jaw. Modern additional diagnostic methods, for example, CBCT and MRI, make it possible to identify a Stafne defect.

[Aspiration test as dental anesthesia safety criterion]. / Aspiratsionnyi test kak kriterii bezopasnosti obezbolivaniya v stomatologii.

Intravascular anesthetic injection can lead to acute toxic reaction even the minimal dose of the drug was administered. The aspiration test is a generally accepted standard for local anesthesia in medicine, specially designed to reduce the risk of emergency conditions. Medical instruments should be adapted for aspiration test, the local anesthetic carpule should have a retention notch, and the dental syringe plunger need to have retention elements. The aspiration test protocol may vary. For legal protection of the doctor the aspiration test result should be described in the patient's medical record.

[Surgical treatment tactics in patients with third molars located close to mandibular canal]. / Taktika lecheniya patsientov pri udalenii tret'ikh molyarov nizhnei chelyusti, prilezhashchikh k nizhnechelyustnomu kanalu.

The tight fit of the roots of the third molars to the mandibular canal is the main predisposing factor of injury to the inferior alveolar nerve during tooth extraction surgery. Surgical treatment of 92 patients diagnosed with pericoronitis, retention of the third molar of the mandible, close fit of the roots of the third molar to the mandibular canal was performed. Four types of close fit of the roots of the third molars to the mandibular canal were revealed, according to CBCT, OPTG and clinical data. Most cases of paresthesia after the removal of the third molars are associated with an anatomical variation in the structure of the mandibular canal, in which the canal walls are partially formed by the cement of the tooth root. Two variants of postoperative management of the surgical wound are proposed, when an open section of the neurovascular bundle is detected at the bottom of the hole.

[Bisphosphonate-related osteonecrosis of the jaws]. / Bisfosfonatnye osteonekrozy chelyustei.

The number of patients with metabolic osteopathies and oncological diseases occurring with the formation of bone metastases is constantly growing and requires special attention not only of oncologists, but also maxillofacial surgeons, dental surgeons and periodontists, due to severe complications from the oral cavity, against the background of antiresorptive therapy with bisphosphonates. These drugs are associated with the development of necrotic processes of the jaw bones and surrounding tissues. It is worth noting the fact that the development of complications after taking these drugs leads to a significant increase in the suffering of patients. The importance of an integrated approach to the treatment and prevention of such complications is extremely important, as it reduces the risk of possible complications and improves the quality of life of this group of patients.

[Optimization of the diagnosis of precancerous diseases of the oral mucosa and lips (Manganotti's abrasive precancerous cheilitis and erythroplakia)]. / Optimizatsiya diagnostiki predrakovykh zabolevanii slizistoi obolochki rta i krasnoi kaimy gub (abrazivnyi prekantseroznyi kheilit Manganotti i eritroplakiya).

OBJECTIVE: To optimize the identification of precancerous diseases of the oral mucosa, such as Manganotti's abrasive precancerous cheilitis and erythroplakia of the oral mucosa using markers Ki-67 and keratin 15. MATERIAL AND METHODS: The study group included 17 patients with Manganotti's abrasive precancerous cheilitis and 8 with erythroplakia of the oral mucosa. A histological analysis was carried out to scrutinize the biopsy material of the oral mucosa of the patients. Ki-67 protein and keratin 15 were used to assess the proliferative activity of epithelial cells and the severity of neoplasia. Results. Thirteen patients with Manganotti's cheilitis had dysplasia of varying degrees, and 4 had signs of malignancy. A high proliferative activity of the Ki-67 protein was found in the stratum basal and stratum spinosum of the epithelium. In 5 patients with erythroplakia, according to histological examination, grade 2-3 intraepithelial neoplasia was revealed and 3 patients had cancer in situ. The expression of keratin 15 in all patients with Manganotti's abrasive precancerous cheilitis and erythroplakia in the basal layer tended to decrease with an increase in the degree of dysplasia, however, the appearance of keratin 15 in the cytoplasm of cells located above the basal layer was discovered. CONCLUSION: The use of Ki-67 protein and keratin 15 as immunohistochemical markers in the study of diseases such as Manganotti's abrasive precancerous cheilitis and erythroplakia of the oral mucosa makes it possible to identify the initial signs of malignancy more accurately in the epithelium.

[Root dislocation into the mouth floor as a complication of lower impacted third molar extraction]. / Khirurgicheskaya taktika pri dislokatsii kornya v myagkie tkani dna polosti rta v khode udaleniya nizhnego retinirovannogo tret'ego molyara.

Root dislocation into soft tissues during lower jaw third molar extraction belongs to clinically rare complications. The thickness of the cortical plate on the third molar lingual side does not exceed 1.5 mm. Working with an elevator, or headpiece, it is possible to push the roots into the adjacent soft tissue. It is not possible to remove displaced tooth fragment through the socket, it requires additional surgical access from the lingual side. The authors described three clinical cases of root dislocation during third molar removing. Successful surgical treatment was done.

[The role of Sonic Hedgehog signaling pathway in the development of oral squamous cell carcinoma]. / Rol' signal'nogo puti Sonic Hedgehog v formirovanii ploskokletochnogo raka slizistoi obolochki rta.

The research of the Sonic Hedgehog signaling pathway activity was carried out on biopsy specimens of 43 patients with oral hyperplasia, squamous intraepithelial neoplasia and oral squamous cell carcinoma. Tissue antigens were determined using a mouse monoclonal antibody to Ki-67 protein and rabbit monoclonal antibody to Gli-1 protein. In squamous intraepithelial neoplasia and oral squamous cell carcinoma specimens Gli-1 was detected in the cytoplasm of cells only. The correlation was found between the proliferative activity of epithelial cells and Gli-1 distribution. Protein Gli-1 can be used for early diagnosis of malignant transformation of oral stratified squamous epithelium.

[The role of Wnt/ß-catenin signaling pathway in ameloblastoma formation]. / Rol' Wnt/ß-kateninsignal'nogo puti v formirovanii ameloblastomy.

The aim of the study was to assess the role of the canonical Wnt/ß-catenin signaling pathway in the formation and clinical course of various histological variants of ameloblastoma. MATERIAL AND METHODS: 76 cases of ameloblastoma were investigated, the average age of the patients was 46 years. There were 49 (64.5%) cases of recurrence of the disease. Tissue antigens were determined using monoclonal rabbit antibodies to Ki-67 and ß-catenin. RESULTS: it was found correlation between the proliferative activity of ameloblastoma cells and the intranuclear localization of ß-catenin, indicating the activation of the Wnt/ß-catenin-signaling pathway. It was shown that the nuclear localization of ß-catenin is positively correlated with the recurrence. CONCLUSION: The Wnt/ß-catenin signaling pathway is involved in a formation and progression of ameloblastoma.

[Diagnostic value of optical coherence tomography for oral leukoplakia assessment]. / Diagnosticheskaia tsennost' metoda kogerentnoi tomografii u bol'nykh s leikoplakiei slizistoi obolochki rta.

The article presents a comparative analysis of the results of in vivo scanning of oral mucosa leukoplakia with optical coherence tomography (OCT) method and pathological studies in 15 patients (9 females and 6 males aged 27-82). The study revealed specific OCT signs of leukoplakia: even disorder of tissue layering and less clear visualization of lamina propria. OCT is an important stage in the differential diagnosis of leukoplakia of the oral mucosa and significantly complements the clinical and pathomorphological study.

[Clinical and radiological features of inflammatory lesions in the lower molars area]. / Kliniko-rentgenologicheskaia kharakteristika vospalitel'no-destruktivnykh protsessov v oblasti moliarov nizhnei cheliusti.

The study objective was to analyze clinical manifestations by various forms of inflammatory destructive lesions in lower molars area. The study included 54 patients aged 20 to 66 years with odontogenic alveolar bone defects in lower molars area. Typical clinical and radiological signs are described for each type of destructive lesions. Sinus tract located more coronal and 'areolar' bone resorption may indicate root perforation. Clinical and radiological features may help in choosing optimal surgical tactic.

[Morphology of epithelial-mesenchymal transformation in neoplasias of oral mucosa]. / Morfologicheskie osnovy épitelial'no-mezenkhimal'noi transformatsii épiteliia slizistoi obolochki rta pri neoplazii.

The aim of the study was to assess correlation of E-cadherin and N-cadherin expression with proliferative activity and neoplasia grade in oral mucosa. Oral biopsies of 48 patients were studied with focal hyperplasia, squamous intraepithelial neoplasia and oral squamous cell carcinoma. Tissue antigens were determined using a mouse monoclonal antibody to Ki-67 and E-cadherin and rabbit polyclonal antibodies to N-cadherin. The study revealed correlation between the proliferative activity of epithelial cells and N-cadherin synthesis associated with decline of E-cadherin expression. Intercellular junction proteins E- and N-cadherins may be used for early diagnosis of malignant transformation of oral squamous epithelium.

[Leukoplakia: clinical and pathological diagnosis]. / Leikoplakiya: klinicheskii i patomorfologicheskii diagnoz.

Leukoplakia is one of the most common obligate precancerous lesions of the oral mucosa. leukoplakia treatment is carried out in conjunction with surgery. Removed tissues are sent to pathological examination. The article describes clinical signs and corresponding pathological processes most often revealed by histological study.

[Prevention of lingual nerve damage by removing stones from submandibular salivary gland duct]. / Profilaktika povrezhdenii yazychnogo nerva pri udalenii konkrementov iz protoka podnizhnechelyustnoi slyunnoi zhelezy.

The aim of the study was to elaborate lingual nerve sparing procedure of submandibular gland duct stones extraction. The study involved 43 patients with syalolithiasis treated in Oral Surgery Unit of Central Research Institute of Dentistry and Maxillofacial Surgery in 2013-2015. It was shown that to prevent lingual nerve and artery injury submandibular salivary gland duct should be dissected to the level of obstruction thus allowing adequate visualization of anatomical correlations especially when removing stones from the distal part of the duct.

[Surgical treatment of patients with oral leukoplakia]. / Khirurgicheskoe lechenie patsientov s leikoplakiei slizistoi obolochki rta.

The aim of the study was to elaborate lingual nerve sparing procedure of submandibular gland duct stones extraction. The study involved 43 patients with syalolithiasis treated in Oral Surgery Unit of Central Research Institute of Dentistry and Maxillofacial Surgery in 2013-2015. It was shown that to prevent lingual nerve and artery injury submandibular salivary gland duct should be dissected to the level of obstruction thus allowing adequate visualization of anatomical correlations especially when removing stones from the distal part of the duct.