Antimicrobial activity of isocytisoside and extracts of Aquilegia vulgaris L.

AIMS: The aim of this study was to analyse the antimicrobial properties extracts of Aquilegia vulgaris, and their principial flavonoid component and to compare the obtained results with the activity of gentamicin and nystatin. METHODS AND RESULTS: The ethanol, acetone and isopropanol extracts as well as the subextracts isolated from the methanol extract together with the main flavonoid: 4'-methoxy-5,7-dihydroxyflavone 6-C-glucoside (isocytisoside) were obtained from the leaves with stems of Aquilegia vulgaris L. All the extracts were analysed by TLC to confirm flavonoids and phenolic acids occurrence. The antimicrobial activity was tested by the method of series dilutions against different Gram-positive, Gram-negative bacteria and also fungi. The results have shown that the extracts, subextracts and isocytisoside inhibit growth of all studied micro-organisms, revealing the greatest activity against Gram-positive Staphylococcus aureus, Staph. epidermidis and the mould Aspergillus niger. CONCLUSIONS: The antimicrobial activity of the tested materials it is possibly related to the content of isocytisoside. SIGNIFICANCE AND IMPACT OF THE STUDY: This study has determined new activity of A. vulgaris and suggested the necessity of further studies.

[Determination of capsaicinoid content after chromatographic separation of peppers]. / Oznaczanie zawartosci kapsaicynoidów po rozdziale chromatograficznym w owocu pieprzowca.

The content of capsaicinoids calculated on capsaicin was determined in the native Fructus Capsici, using the methods described in FP IV, DAB 7 and Ph. Hungarica 7. All modifications which were introduced allow on the performance of the analysis according to the equipment of laboratories and also in drugs containing various content of capsaicin. The statistical estimations which were carried on give evidence that obtained results are reproducible. The presented methods can be applied to serial analysis.