Adaptive changes of the electrophoretic mobility of cell nuclei (EMN) index in the intensive physical exercise of male rowers with different training experience.

The aim of this study is to attempt to determine the relationship between the degree of the EMN index and the depth of changes of selected hormones and metabolic parameters as an effect of intensive physical exercise during the training process in male rowers. Juniors (N = 62; chronological age 16.4 y.o. SD = 1.14 y.; training experience 3.0 y.o. SD = 1.05 y.) and seniors (N = 27; chronological age 21.4 y. SD = 1.73 y.; training experience 5.5 y.o. SD = 1.10 y.), in the preparatory period of the training process, performed physical exercise of maximum intensity on a rowing ergometer. Acid-base balance parameters (pH, BE) and the concentration of lactic acid (LA) were determined as the result of physical exercise. Some selected hormones were also indicated (hGH, PrL and Prg) to show their exercise changes. A sample of buccal epithelium cells was taken from each of the male rowers, before and after the exercise, to evaluate the percentage of the EMN index by intracellular microelectrophoresis. A greater differentiation of metabolic changes during exercise was manifested in juniors than seniors. This was reflected in changes of acid-base balance parameters, exercise physiological parameters, hormone concentration and also in changes of the EMN index. These changes were probably dependent on deep metabolic processes of an acid character during exercise. This could prove a more stable homeostasis through more economical metabolic reactions in seniors as the effect of the training process, meaning that seniors were better adapted to heavy physical exercise than juniors.

The Electrophoretic Mobility of Cell Nuclei (EMN) index and changes in acid-base homeostasis under conditions of the intensive physical exercise.

The relationship between the Electrophoretic Mobility of Cell Nuclei (EMN) index before and after super-maximal and maximal exercises and changes in physiological parameters associated with acid-base balance of the blood (pH, BE) and lactic acid concentration (LA) were examined in junior (N = 33, X = 15.6 y.o. SD = 1.16 y.), and senior (N = 10, X = 22.0 y.o. SD = 2.70 y.) female rowers. The following parameters: pH, BE and LA changed significantly (p < 0.05) from pre- to post-exercise status, while the EMN index changed only in junior female rowers under super-maximal conditions. Correlations between the EMN index and physiological parameters reflect the homeostatic disturbance associated with intensive exercise conditions. The decline in the EMN index appears to depend on the post-exercise changes of an organism's acidity. The results suggest that changes in the EMN index are associated with variation in physiological parameter, i.e. changes in acidity. We conclude that the EMN index reflects acid-base alterations and may be useful in evaluating systematic reactions to stress.