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Sci Rep ; 7: 44628, 2017 03 16.
Artigo em Inglês | MEDLINE | ID: mdl-28300169

RESUMO

Serial femtosecond crystallography requires reliable and efficient delivery of fresh crystals across the beam of an X-ray free-electron laser over the course of an experiment. We introduce a double-flow focusing nozzle to meet this challenge, with significantly reduced sample consumption, while improving jet stability over previous generations of nozzles. We demonstrate its use to determine the first room-temperature structure of RNA polymerase II at high resolution, revealing new structural details. Moreover, the double-flow focusing nozzles were successfully tested with three other protein samples and the first room temperature structure of an extradiol ring-cleaving dioxygenase was solved by utilizing the improved operation and characteristics of these devices [corrected].


Assuntos
Cristalografia/instrumentação , Reologia/instrumentação , Simulação por Computador , RNA Polimerase II/química , Saccharomyces cerevisiae/enzimologia , Temperatura , Fatores de Tempo , Difração de Raios X
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