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1.
Rev Sci Tech ; 3(3): 465-525, 1984 Sep.
Artigo em Espanhol, Francês, Inglês | MEDLINE | ID: mdl-32988001
5.
Acta Microbiol Acad Sci Hung ; 23(1): 23-8, 1976.
Artigo em Inglês | MEDLINE | ID: mdl-59518

RESUMO

Purified protein derivative from Mycobacterium bovis was separated into 6 fractions by electrophoresis at 1500 V/40 mA in pH 4.2 acetic acid-pyridine buffer. Further purification of one of the fractions on Sephadex G 25 column and by acid hydrolysis yielded antigen "PS" eliciting tuberculin reaction only in animals vaccinated with M. bovis BCG but not in those sensitized with M. avium and some fast-growing mycobacteria. The specificity of antigen "PS" was confirmed in vitro: the antigen induced blastic transformation only in lymphocytes of guinea pigs sensitized with M. bovis BCG.


Assuntos
Mycobacterium bovis/imunologia , Tuberculina/análise , Animais , Bovinos , Fracionamento Químico , Epitopos , Cobaias/imunologia , Ativação Linfocitária , Mycobacterium avium/imunologia , Teste Tuberculínico , Tuberculose Bovina/imunologia
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