RESUMO
This study reviewed the records of all new patients seen in an outpatient wound-care programme during a six-month period. Most of the 115 patients were referred by physicians in internal medicine or family practice (48%) or surgeons (39%); 101 had a wound (88%). Of the 92 who had their wound measured at the time of their first clinic visit, 72 (78%) had their largest wound located on a lower extremity and 40% of all lower extremity wounds were on the feet. Wounds were most frequently due to pressure (36%), venous insufficiency (16%), diabetic neuropathy (16%), or arterial insufficiency (13%). Within 12 months of their initial presentation, wound closure was documented in 35 of the 92 patients (38%). Of the four most common aetiologies, the healing rate was highest in wounds attributable to venous insufficiency (67%) (p < 0.02).
Assuntos
Assistência Ambulatorial/organização & administração , Equipe de Assistência ao Paciente/organização & administração , Ferimentos e Lesões/terapia , Adulto , Idoso , Idoso de 80 Anos ou mais , Doença Crônica , Humanos , Auditoria Médica , Pessoa de Meia-Idade , Avaliação de Programas e Projetos de Saúde , Estudos Retrospectivos , Resultado do Tratamento , Cicatrização , Ferimentos e Lesões/etiologiaRESUMO
This study reviewed the records of all new patients seen in an outpatient wound-care programme during a six-month period. Most of the 115 patients were referred by physicians in internal medicine or family practice (48%) or surgeons (39%); 101 had a wound (88%). Of the 92 who had their wound measured at the time of their first clinic visit, 72 (78%) had their largest wound located on a lower extremity and 40% of all lower extremity wounds were on the feet. Wounds were most frequently due to pressure (36%), venous insufficiency (16%), diabetic neuropathy (16%), or arterial insufficiency (1 3%). Within 12 months of their initial presentation, wound closure was documented in 35 of the 92 patients (38%). Of the four most common aetiologies, the healing rate was highest in wounds attributable to venous insufficiency (67%) (p < 0.02).
RESUMO
The oxysterol content in normal and sickle red blood cell (RBC) membranes was assessed using thin-layer chromatography and capillary gas chromatography/mass spectrometry. Several more oxysterols were present in sickle RBCs compared to normal RBCs. Sickle RBC membranes had a higher concentration of 5 alpha,6 alpha-epoxycholesterol, 5 alpha-cholestane-3 beta,5,6 beta-triol, 7-ketocholesterol and 19-hydroxycholesterol than normal RBC membranes. The increased oxysterols in sickle RBC may be an effect of the increased oxidative stress which occurs in sickle RBC membranes. Physical characteristics of normal and sickle RBC membrane ghosts with and without inserted oxysterols were examined by Fourier transform infrared spectroscopy. The data are consistent with a greater sterol content in sickle cells compared to normal RBC membranes, and a possible oxysterol-cholesterol synergism.
Assuntos
Colesterol/sangue , Eritrócitos/metabolismo , Traço Falciforme/sangue , Cromatografia em Camada Fina , Análise de Fourier , Cromatografia Gasosa-Espectrometria de Massas , Humanos , Oxirredução , Espectrofotometria InfravermelhoRESUMO
Fourier transform infrared (FTIR) spectroscopy was used to examine the effect of oxysterol insertion into normal and sickle RBC membranes and the total lipid extracts of the membranes. Examination of the FTIR C-H stretch and fingerprint regions reveal that the insertion of 7 alpha- and 7 beta-hydroxycholesterol has the greatest effect on the fluidity of RBC membranes and lipid extracts. The results confirm the observation that sterol molecules are oriented in the membrane so that the 7 position is located in the phospholipid head group region at the lipid/water interface. The substitution of a keto for a hydroxy group at the number seven position decreases the effect of the sterol on membrane packing.