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1.
Physiol Int ; 110(1): 46-51, 2023 Mar 10.
Artigo em Inglês | MEDLINE | ID: mdl-36753299

RESUMO

Purpose: According to current protocol, the separation of pancreatic head and body is performed at the level of superior mesenteric vein (SMV). Previous data indicate that the resection plane should be modified in portal annular pancreas. We presumed that the optimal line of pancreatic resections could also be different in other cases. Our aim is to simulate pancreatic resections in different planes and find the optimal resection line with the minimum number of cut vessels. Main methods: 25 abdominal vascular corrosion casts were prepared, the aorta and the portal vein were cannulated. CT scans were taken on the casts, and specific planes were reconstructed simulating different resection lines. The total amount of cross sections of vessels were calculated in the different planes. Results: In our series, the optimal plane is the SMV in 11/25, 2 cm left in 10/25, 1 cm left in 4/25, 1 cm right in 1/25 and 2 cm right in none of our cases. The group of left sided extension contain more than half of the cases. With left sided resections, the cut surface of the vessels may be lowered to even 29% compared to the SMV plane. Conclusion: Our study revealed that pancreatic resections should be extended to the left side of the SMV in more than half of our cases. Therefore, the resection plane should be determined by preoperative imaging methods. Using DICOM viewer with multiplanar reconstruction, the resection planes can be simulated in clinical practice, which would reduce the risk of postoperative bleeding.


Assuntos
Pancreatopatias , Neoplasias Pancreáticas , Humanos , Pancreaticoduodenectomia/métodos , Neoplasias Pancreáticas/cirurgia , Pâncreas/cirurgia , Pancreatopatias/cirurgia
3.
J Hepatol ; 66(4): 778-786, 2017 04.
Artigo em Inglês | MEDLINE | ID: mdl-27913222

RESUMO

BACKGROUND & AIMS: In advanced cirrhosis new hepatocytic nodules are generated by budding of ductules in areas of parenchymal extinction. However, the vascular alterations in the areas of parenchymal extinction, the blood supply and the structure of the new hepatocytic nodules have not been analyzed in detail. METHODS: Explanted human cirrhotic livers of three different etiologies and two experimental rat models of cirrhosis were thoroughly examined. 3D reconstruction of the immunohistochemically stained serial sections and casting of human and experimental cirrhotic livers have been used to reveal the structural organization of the regenerative buds. RESULTS: In areas of parenchymal extinction the skeleton of the liver, the portal tree is preserved. The developing regenerative nodules are positioned along the portal tree and are directly supplied by terminal portal venules. The expanding nodules grow along the trunks of the portal vein. Casting of human and experimental cirrhotic livers by colored resin confirms that nodules are supplied by portal blood. The two other members of the portal triads become separated from the portal veins. CONCLUSIONS: As the structure of the hepatocyte nodules (centrally located portal vein branches, bile ducts at the periphery, hepatic veins and arteries in the connective tissue) impedes the restoration of normal liver structure, the basic architecture of hepatic tissue suffers permanent damage. We suggest that "budding" may initiate the second, irreversible stage of cirrhosis. LAY SUMMARY: Cirrhosis is the final common outcome of long lasting hepatic injury defined as the destruction of the normal liver architecture by scar tissue. In the late phase of cirrhosis stem cells-derived hepatocyte nodules appear along the branches of the portal vein suggesting an important role of this specially composed blood vessels (containing digestive end-products from the stomach and intestines) in liver regeneration. Our results contribute to a better understanding of this serious liver disease.


Assuntos
Cirrose Hepática/patologia , Regeneração Hepática/fisiologia , Veia Porta/patologia , Animais , Hepatócitos/patologia , Humanos , Processamento de Imagem Assistida por Computador , Imageamento Tridimensional , Circulação Hepática , Cirrose Hepática/fisiopatologia , Cirrose Hepática Experimental/patologia , Cirrose Hepática Experimental/fisiopatologia , Masculino , Modelos Anatômicos , Neovascularização Fisiológica , Ratos , Ratos Endogâmicos F344
4.
Transpl Int ; 28(10): 1216-26, 2015 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-26152659

RESUMO

The purpose of our study was to investigate the anatomical variations of the extrahepatic arterial structures of the liver with particular attention to rare variations and their potential impact on liver surgery. A total of 50 human abdominal organ complexes were used to prepare corrosion casts. A multicomponent resin mixture was injected into the abdominal aorta. The portal vein was injected with a different colored resin in 16 cases. Digestion of soft tissues was achieved using cc. KOH solution at 60-65 °C. Extrahepatic arterial variations were classified according to Michels. All specimens underwent 3D volumetric CT reconstruction. Normal anatomy was seen in 42% of cases, and variants were seen in the other 58%. No Michels type VI or X variations were present; however, in 18% of cases the extrahepatic arterial anatomy did not fit into Michels' classification. We report four new extrahepatic arterial variations. In contrast to the available data, normal anatomy was found much less frequently, whereas the prevalence of unclassified arterial variations was higher. We detected four previously unknown variations. Our data may contribute to the reduction of complications during surgical and radiological interventions in the upper abdomen.


Assuntos
Artérias/anormalidades , Fígado/irrigação sanguínea , Angiografia , Aorta Abdominal/anatomia & histologia , Classificação , Anormalidades Congênitas/epidemiologia , Molde por Corrosão , Artéria Hepática/anormalidades , Artéria Hepática/diagnóstico por imagem , Humanos , Imageamento Tridimensional , Fígado/cirurgia , Artéria Mesentérica Superior/anormalidades , Artéria Mesentérica Superior/diagnóstico por imagem , Veia Porta/anatomia & histologia , Prevalência , Tomografia Computadorizada por Raios X
5.
Pediatr Transplant ; 19(5): 510-6, 2015 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-25907302

RESUMO

Multiple duct anastomoses during LLS transplantation increase the incidence of biliary complications. The optimal plane of hepatotomy that results in the least number of bile ducts at the surface was investigated according to LHD variations. Ducts of 30 human livers were injected with resin and LHD branching on 3D-CT reconstructions were analyzed. Ducts on the virtual hepatotomy surface were estimated in three splitting lines. Variations with subtypes were described. Ia (66.7%): ducts from segments (S.) II-III form a common trunk and S.IV duct joins it. Ib (10%): common trunk formed by ducts from S.II-S.III while S.IV duct joins the common hepatic duct. IIa (16.67%): S.IV duct drains into S.III duct. IIc (3.33%): S.IV duct drains into both S.II and S.III ducts. III (3.33%): trifurcation of S.II, S.III and S.IV ducts. When the virtual hepatotomy line was on the FL, there was a single duct for the anastomosis in 30% of cases but two, three, or four ducts in 53.3%, 10%, and 3.3%, respectively. Division 1 cm to the right of the FL resulted in one duct (70%), but S.IV duct injury may occur. LLS hepatotomy should not necessarily be performed along the FL. Variations must be taken into consideration to minimize the number of biliary anastomoses during liver implantation.


Assuntos
Ductos Biliares/cirurgia , Hepatectomia/métodos , Ducto Hepático Comum/cirurgia , Fígado/anatomia & histologia , Doadores Vivos , Adulto , Anastomose Cirúrgica , Autopsia , Ductos Biliares/anatomia & histologia , Procedimentos Cirúrgicos do Sistema Biliar , Colangiografia/métodos , Ducto Hepático Comum/anatomia & histologia , Humanos , Imageamento Tridimensional , Fígado/cirurgia , Transplante de Fígado , Pâncreas/anatomia & histologia , Tomografia Computadorizada por Raios X
6.
Surg Today ; 44(12): 2345-53, 2014 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-24374367

RESUMO

PURPOSE: Rats are the most commonly used animal model for studies of acute lower limb ischemia-reperfusion. The ischemia induced by arterial clamping may cause milder damage than the application of a tourniquet if the presence of a possible collateral system is considered. METHODS: Male Wistar rats were randomized into three groups: in group A, the muscle weight affected by ischemia was measured; in group B, the severity of muscle damage caused by the application of a tourniquet and by infrarenal aortic occlusion was examined. Blood and muscle samples were taken from group B to assess the serum necroenzyme, potassium and TNF-α levels, as well as the muscle fiber viability and for histological examinations. In group C, the identification of the lower limb collateral system was performed using corrosion casting. RESULTS: Tourniquet application affected the lower muscle mass and resulted in significantly more severe injury compared to infrarenal aortic occlusion. This difference was reflected in the serum necroenzyme, potassium and TNF-α levels. The histological examination and viability assay confirmed these findings. The corrosion casts showed several anastomoses capable of supplying the lower limb. CONCLUSION: Tourniquet application proved to be capable of inducing absolute lower limb ischemia, in contrast to infrarenal aortic ligation, where a rich collateral system is considered to help mitigate the injury.


Assuntos
Aorta , Circulação Colateral/fisiologia , Isquemia/fisiopatologia , Extremidade Inferior/irrigação sanguínea , Traumatismo por Reperfusão/fisiopatologia , Reperfusão , Animais , Arteriopatias Oclusivas/patologia , Arteriopatias Oclusivas/fisiopatologia , Modelos Animais de Doenças , Isquemia/patologia , Rim/irrigação sanguínea , Ligadura , Masculino , Ratos Wistar , Traumatismo por Reperfusão/patologia , Torniquetes
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