RESUMO
Serum autoantibodies to visual arrestin, also termed S-antigen, have been shown to accompany several autoimmune-related diseases. However, they were also detected in sera of healthy individuals; there is lack of a sensitive and fast method for evaluation of putative differences between those two groups of antibodies. We show that, using biosensor technology based on surface plasmon resonance (SPR), it was possible to characterize real-time interactions of immune sera with immobilized arrestin. Binding characteristics revealed different interaction kinetics of antiarrestin antibodies present in two distinct rabbit sera and, thus, broadened results of immunoblotting analysis. Therefore, we suggest that SPR-based biosensor technology might be a valuable method for monitoring and evaluation of antiarrestin antibodies in patients' sera.
Assuntos
Arrestina/imunologia , Autoanticorpos/sangue , Ressonância de Plasmônio de Superfície/métodos , Animais , Complexo Antígeno-Anticorpo/análise , Reações Antígeno-Anticorpo , Técnicas Biossensoriais , Bovinos , Humanos , Imunoglobulina G/química , Imunoglobulina G/isolamento & purificação , Coelhos , Sensibilidade e Especificidade , Fatores de TempoRESUMO
Arrestins are cytosolic proteins involved in the termination of signaling by activated G protein-coupled receptors (GPCR). Four different arrestins are identified in mammals to date. Two of these are specific to retinal photoreceptor cells, while the other two (beta-arrestin 1 and 2) are ubiquitously expressed. Recently, several new regulatory functions of arrestins in such processes as receptor ubiquitination, signaling through kinases, and others have been discovered.
