Chemical composition of Lavatera thuringiaca L. biomass ash after pre-sowing stimulation of seeds with He-Ne laser light.

The article presents the effect of pre-sowing Lavatera thuringiaca L. seeds stimulation with He-Ne laser light on the chemical composition (P, S, K, Ca, Mn, Fe, Ni, Cu and Zn content) of ash obtained after combustion of shoots of different ages. Results varied, but it was confirmed that pre-sowing exposure of seeds to this physical factor for 10-min has the most pronounced effect on K, Cu and Mn content increase (6%, 20%, 31% increase respectively) in the ash after the first year of vegetation as well as on Cu, K and Zn content increase (9%, 19%, 22% increase respectively) after the second year of vegetation. However, 30-min stimulation significantly increases Ca (8%), Mn (20%) and Fe (72%) content in ash after the second year as well as results in ash richer in Ca (22%), P (48%), K (70%) and Zn (95%) after the third year of Lavatera vegetation. The pre-sowing application of He-Ne laser light depending on the time of stimulation can intensify the content of preferable macro- and microelement groups in Lavatera ash, in respective cultivation years. It can be an innovative method of biomass ash enhancement and its more effective use in agriculture as commercial fertilizers substitute.

Ecotoxicity of soil contaminated with diesel fuel and biodiesel.

Fuels and their components accumulate in soil, and many soil organisms are exposed to this pollution. Compared to intensive research on the effect of conventional fuel on soil, very few studies have been conducted on soil ecotoxicity of biofuels. Considering the limited information available, the present study evaluated the changes caused by the presence of biodiesel and diesel fuel in soil. The reaction of higher plants and soil organisms (microbial communities and invertebrates) was analysed. Conventional diesel oil and two types of biodiesel (commercial and laboratory-made) were introduced into the soil. Two levels of contamination were applied-5 and 15% (w/w per dry matter of soil). The plate method was used to enumerate microorganisms from soil contaminated with biodiesel and diesel fuel. Phytotoxicity tests were conducted by a 3-day bioassay based on the seed germination and root growth of higher plant species (Sorghum saccharatum and Sinapis alba). Fourteen-day ecotoxicity tests on earthworm were performed using Eisenia fetida. Based on the results of the conducted tests it was found out that the organisms reacted to the presence of fuels in a diverse manner. As to the microorganisms, both the growth and reduction of their number were noted. The reaction depended on the group of microorganisms, type of fuel and dose of contamination. The lipolytic and amylolytic microorganisms as well as Pseudomonas fluorescens bacteria were particularly sensitive to the presence of fuels, especially biodiesel. Fuels, even at a high dose, stimulated the growth of fungi. Monocotyledonous sugar sorghum plants were more sensitive to the presence of fuels than dicotyledonous white mustard. There was also a significant negative impact of contamination level on plant growth and development. Biodiesel, to a greater extent than conventional fuel, adversely affected the survival and volume of earthworms.