Colorimetric Fluorescent Sensors for Hemoglobin Based on BODIPY Dyes.

Colorimetric fluorescent chemosensors 4 and 5 based on mono- and di- styryl borondipyrromethenes (BODIPY) linked methyl malonyl were designed for detection of hemoglobin (HgB). Their sensing behavior toward various analytes (Br-, EDTA, Glucose, CO32-, Fe2+, Fe3+, I-, NO3-, PO43-, SO42-,Cl-, Urea, K+, Mg2+, Na+, NH4+, Zn2+,Ca2+,Cd2+, Li+, Pb2+, Cs2+, Ag+, Mn2+, Cr2+, Ni2+, Hg2+, Al3+) were investigated by fluorescence spectroscopies. Addition of HgB to acetone: water (4:1) solutions of BODIPYs 4 and 5 solutions gave visual color changes, as well as significantly quenched fluorescence emissions, while other analytes induced no or much smaller spectral changes. The sensing method for both BODIPYs 4 and 5 was successfully applied to measure the HgB in human blood with satisfactory results. Spike and recovery tests in human blood samples exhibit good recovery rates for the spiked concentrations close to the limit of detection. It was found that BODIPYs 4 and 5 constituted the HgB selective fluorescent chemosensor (ON-OFF) and the detection limits were calculated to be 1.773 µg. mL-1 and 1. 295 µg.mL-1 respectively.

Hexa-BODIPY Linked-Triazole Based on a Cyclotriphosphazene Core as a Highly Selective and Sensitive Fluorescent Sensor for Fe(2+) Ions.

A new type of fluorescent chemosensor based on tethered hexa-borondipyrromethene cyclotriphosphazene platform (HBTC) linked via triazole groups was designed and synthesized. Its sensing behavior toward metal ions was investigated by ultraviolet-visible and fluorescence spectroscopies. Addition of a Fe(2+) ion to a tetrahydrofuran solution of HBTC gave a visual color change as well as a significantly quenched fluorescence emission, while other tested 19 metal ions induced no color or spectral changes. This compound was found to be highly selective and sensitive for Fe(2+) with a low limit of detection (2.03 µM) which is, to the best of our knowledge, the superior than the previously studied chemosensors for Fe(2+). Graphical Abstract ᅟ.