Label-free image scanning microscopy for kHz super-resolution imaging and single particle tracking.

We report the modification of a label-free image scanning microscope (ISM) to perform asynchronous 2D imaging at up to 24kHz while keeping the lateral resolution gain and background rejection of a regular label-free ISM setup. Our method uses a resonant mirror oscillating at 12kHz for one-direction scanning and a chromatic line for instantaneous scanning in the other direction. We adapt optical photon reassignment in this scanning regime to perform fully optical super-resolution imaging. We exploit the kHz imaging capabilities of this confocal imaging system for single nanoparticle tracking down to 20 nm for gold and 50 nm for silica particles as well as imaging freely moving Lactobacillus with improved resolution.

Ten simple rules for designing and running a computing minor for bio/chem students.

Science students increasingly need programming and data science skills to be competitive in the modern workforce. However, at our university (San Francisco State University), until recently, almost no biology, biochemistry, and chemistry students (from here bio/chem students) completed a minor in computer science. To change this, a new minor in computing applications, which is informally known as the Promoting Inclusivity in Computing (PINC) minor, was established in 2016. Here, we present the lessons we learned from our experience in a set of 10 rules. The first 3 rules focus on setting up the program so that it interests students in biology, chemistry, and biochemistry. Rules 4 through 8 focus on how the classes of the program are taught to make them interesting for our students and to provide the students with the support they need. The last 2 rules are about what happens "behind the scenes" of running a program with many people from several departments involved.

Transmission matrix approaches for nonlinear fluorescence excitation through multiple scattering media.

Several matrix approaches were developed to control light propagation through multiple scattering media under illumination of ultrashort pulses of light. These matrices can be recorded with either spectral or temporal resolution. Thanks to wavefront shaping, temporal and spatial refocusing has been demonstrated. In this Letter, we study how these different methods can be exploited to enhance a two-photon excitation fluorescence process. We first compare the different techniques on micrometer-size isolated fluorescent beads. We then demonstrate point-scanning imaging of these fluorescent microbeads located after a thick scattering medium at a depth where conventional imaging would be impossible because of scattering effects.