In Vitro Effect of Photobiomodulation Therapy with 980 nm Diode Laser on Gene Expression of Key Regulators of Bone Remodeling by Human Periodontal Ligament Cells under Mild Orthodontic Forces.

This study investigated the effect of photobiomodulation (PBM) with 980 nm diode laser as monotherapy and in combination with compressive and tensile orthodontic forces on expression of osteoprotegerin (OPG), receptor activator of nuclear factor-κB ligand (RANKL), sclerostin (SOST) and periostin (POSTN), by human periodontal ligament cells. Isolated cells were cultured and subjected to either tensile (10% elongation) or compressive forces (25 g cm-2 ) for 24 and 48 h. Subsequently, the cells received PBM (100 mW power, 3 or 6 J cm-2 energy density) immediately after load cycle. RT-PCR was applied to assess the genes expression. Data were analyzed by one-way ANOVA, followed by post hoc Tukey test (P ≤ 0.05). We found that PBM in combination with orthodontic forces led to upregulation of bone resorption genes (RANKL and SOST) at the pressure side and their downregulation at the tension side. The expression of osteogenic genes (OPG and POSTN) increased at the tension side and decreased at the pressure side. PBM alone did not affect gene expression. In conclusion, these findings suggest that this PBM protocol may be effective in enhancement of the gene expression in favor of bone remodeling acceleration that should be confirmed in future animal and human studies.

COVID-19 vaccination and psychological status of Iranian dental students.

Objective: The purpose of the present study was to investigate the association of COVID-19 vaccination with the quality and quantity of sleep, the level of stress, and temporomandibular joint (TMJ) disorders (TMDs) in Iranian dental students. Materials and methods: In this cross-sectional research, we applied a questionnaire including 30 questions on the Perceived Stress Scale (PSS), sleep quality and quantity, Diagnostic Criteria for Temporomandibular Disorders (DC/TMD), and vaccination status. All vaccinated students of the dental schools located in the city of Tehran were invited to participate in the study. Participants were divided into three groups: those vaccinated for less than a month, those vaccinated for 1-3 months, and those vaccinated for more than 3 months. A paired t-test served for statistical analysis. Results: Overall, 171 out of 235 students (72.77%) completed the questionnaire, among which 90 individuals were fully vaccinated, and were included in the data analysis. Stress levels decreased (mean difference = -1.23, p-value = 0.002) and sleep quality and quantity improved mostly 1-3 months after the vaccination (mean difference = -0.5, p-value = 0.016). However, TMD symptoms were mostly alleviated in people vaccinated for more than 3 months (mean difference = -2.86, p-value <0.05). In this respect, no significant difference was observed between the two genders. Conclusion: According to the results of the study, vaccination was associated with the improvement of psychological consequences of the COVID-19 pandemic. It is recommended that further longitudinal studies be conducted on larger sample sizes and different age groups by using various data collection methods (especially regarding the assessment of TMD).

Evaluation of Compressive Strength of Several Pulp Capping Materials.

STATEMENT OF THE PROBLEM: Adequate compressive strength is an important characteristic for an ideal liner. PURPOSE: This study aimed to assess the compressive strength of several commonly used liners. MATERIALS AND METHOD: This in vitro, experimental study evaluated 120 samples fabricated of Dycal, Calcimol LC, Vitrebond, Activa Bioactive, and TheraCal LC (n=24) liners according to the manufacturers' instructions. The samples were fabricated using a cylindrical stainless steel mold with 6±0.1 mm height and 4±0.1 mm internal diameter. Half of the samples in each group (n=12) underwent compressive strength test immediately after completion of their primary setting while the other half (n=12) underwent compressive strength test after 24 h. During this period, the samples were immersed in deionized water (grade 3) and incubated at 37±1°C and 100% humidity for 24 h. The compressive strength was measured using a universal testing machine. Data were analyzed using two-way ANOVA followed by Tukey's post-hoc test. RESULTS: The compressive strength of the five liners was significantly different (p< 0.05). Calcimol LC showed maximum compressive strength both immediately after setting and after 24 h. The compressive strength at 24 h was significantly higher than the primary compressive strength in all groups (p< 0.05). CONCLUSION: Within the limitations of this study, it seems that Calcimol LC, Activa Bioactive Liner, and TheraCal LC have adequate compressive strength and can be used alone to provide adequate support for the restorative materials.