Expression of the Annexin A1 and its correlation with matrix metalloproteinases and the receptor for formylated peptide-2 in diffuse astrocytic tumors.

Astrocytomas represent the majority of cerebral gliomas. Studies show that the anti-inflammatory protein Annexin-A1 (ANXA1) is associated with the tumor invasion process and that its actions can be mediated by the receptor for formylated peptides (FPR). Therefore, we evaluated the expression of ANXA1, the receptor FPR2 and matrix metalloproteinases 2 and 9 (MMP-2 and MMP-9) in brain astrocytomas. Detection of proteins was performed in sections of diffuse astrocytomas (grade II), anaplastic astrocytomas (grade III) and glioblastomas (GBM, grade IV) and quantifications were made by densitometry. Our analyses showed increased expression of ANXA1 in astrocytomas of all grades, but especially in GBM. The expression of FPR2 is similar to that found for ANXA1, being higher in GBM. Immunostaining for MMPs is also stronger as the degree of malignancy increases, especially with respect to MMP-9. The positive correlation between ANXA1/FPR2 and ANXA1/MMP-9 was observed in all tumors studied. The data indicate the possible action of ANXA1 and FPR2 on the development and progression of astrocytomas, related to increased expression of MMP-9. Thereby, ANXA1 and FPR2 are involved in the biology and malignancy of diffuse astrocytic tumors.

Expressão de Metaloproteinases de Matriz e Anexina A1 em tumores de pele de diferentes graus de malignidade / Expresión de Metaloproteinasas de Matriz y Anexina A1 en tumores de la piel de diferentes grados de malignidade / Expression of the Matrix Metalloproteinases and Annexin A1 in skin tumors of different malignancy grades

Introdução: Câncer de pele é doença multifatorial consequente de alterações genéticas e exposição prolongada aos raios solares, entreoutras causas. Os basocarcinomas (CBC) são benignos, com invasividade local e reduzido potencial de metastatização; os carcinomasespinocelulares (CEC) são neoplasias malignas, divididos nos graus 1, 2 e 3 e no grupo acantolítico, de pior prognóstico. Estudos associama resposta inflamatória exacerbada e modificação na matriz extracelular com o desenvolvimento tumoral. Objetivos: Analisar, por imunohistoquímica,nas lesões de CBC e CEC (1, 2, 3 e no subtipo acantolítico) a expressão da proteína anti-inflamatória anexina A1 (ANXA1)e das metaloproteinases de matriz dos tipos 2 e 9 (MMP2 e MMP9). Material e Método: Para as análises imuno-histoquímicas, secçõesdas biópsias de todos os grupos, após recuperação antigênica e bloqueio da atividade da peroxidase endógena foram incubadas comanticorpos primários policlonais rabbit: anti-ANXA1 (1:2000), anti-MMP2 e anti-MMP9 (1:100). Posteriormente, incubadas com o anticorposecundário biotinilado, reveladas com os substratos diaminobenzidina ou o aminoetil carbazol e contracorados com hematoxilina. Aexpressão das...

Introduction: Skin cancer is a multifactorial disease resulting from genetic mutations as well as from prolonged exposure to sunlight,among other causes. Basal cell carcinomas (BCC) are benign, with local invasiveness and reduced potential to metastasize, while,squamous cell carcinoma (SCC) are malignant neoplasms, and can be divided into grades 1, 2 and 3, besides of the acantholytic group,which presents poor prognosis. Studies associate exacerbated inflammatory response and modification in the extracellular matrix to tumordevelopment. Objectives: To analyze, by immunohistochemistry, the expression of the anti-inflammatory protein annexin A1 (ANXA1) andthe matrix metalloproteinases of the types 2 and 9 (MMP2 and MMP9), in lesions of BCC and SCC (grades 1, 2, 3 and acantholytic subtype).Materials and Method: For immune-histochemical analysis, sections of biopsies of all groups, following antigen retrieval and blocking ofendogenous peroxidase activity were incubated with primary rabbit polyclonal antibodies: anti-ANXA1 (1:2000), anti-MMP2 and antiMMP9(1:100). Subsequently, they were incubated with biotinylated secondary antibodies, developed with diaminobenzidine or aminoethyl carbazole substrate and counterstained with hematoxylin. The expression of the proteins was verified by densitometry and statisticalanalysis by ANOVA and Bonferroni...

Introducción: El cáncer de piel es una enfermedad multifactorial que resulta de cambios genéticos y la exposición prolongada a laluz solar, entre otras cosas. Los basocarcinomas (CBC) son benignos, con invasión local y reducido potencial para la metástasis; loscarcinomas de células escamosas (SCC) son tumores malignos divididos en grados 1, 2 y 3 y el grupo acantolítico, de peor pronóstico.Estudios asocian la respuesta inflamatória exacerbada y la modificación en la matriz extracelular en el desarrollo tumoral. Objetivos:Analizar, mediante técnicas de inmunohistoquímica, en las lesiones de BCC y SCC (1, 2, 3 y acantolítico subtipo) la expresión de antiinflamatorioproteína anexina A1 (ANXA1) y metaloproteinasas de la matriz de tipos 2 y 9 (MMP2 y MMP9). Material y Método...