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1.
Anat Sci Int ; 99(3): 320-325, 2024 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-38557948

RESUMO

This case report describes the coexistence of a retroesophageal right subclavian artery and left maxillary artery which passed deep to the mandibular nerve. An 88-year-old woman died of acute heart failure, and the postmortem revealed that the right subclavian artery originated from the aortic arch as the last branch at the level of the fourth thoracic vertebra, then passed between the esophagus and the vertebral column. The artery then ascended right superiorly and passed behind the anterior scalene muscle. The right vertebral artery arose from the retroesophageal right subclavian artery and entered the transverse foramen of the sixth cervical vertebra. The left maxillary artery branched at the common trunk of the posterior deep temporal and the inferior alveolar arteries. The maxillary artery then turned anteromedially and branched to give the middle meningeal artery. The mandibular nerve gave off the buccal nerve, deep temporal nerve and a thick nerve just below the foramen ovale. The auriculotemporal nerve that branched from the thick nerve ran deep to the maxillary artery. The maxillary artery turned anteriorly, passing deep to the branches. The artery then split to give the buccal artery and the anterior deep temporal artery. In the pterygopalatine section, the maxillary artery branched off to form the common trunk of the infraorbital and sphenopalatine arteries and the posterior superior alveolar artery. It may be necessary to pay attention to the course of the maxillary artery and its relationship to the mandibular nerve branches, when a retroesophageal right subclavian artery is seen.


Assuntos
Nervo Mandibular , Artéria Maxilar , Artéria Subclávia , Humanos , Artéria Subclávia/anormalidades , Feminino , Idoso de 80 Anos ou mais , Artéria Maxilar/anormalidades , Cadáver , Esôfago/irrigação sanguínea , Esôfago/anormalidades , Esôfago/inervação
2.
Mol Pain ; 14: 1744806918804439, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30209980

RESUMO

Transient receptor potential vanilloid 1 (TRPV1) is a nonselective cation channel that is expressed in the sensory neurons and responds to various noxious stimuli including heat and capsaicin. The molecular properties of TRPV1 have been clearly examined; however, there are obvious individual differences in human sensitivity to thermal stimuli and capsaicin. Here, we examined the possibility that different genome sequence of human TRPV1 caused the different sensitivity to heat or capsaicin. The sensitivities to burning pain and capsaicin of Japanese adult subjects were compared with their TRPV1 genome sequence, and we detected 6 single-nucleotide polymorphisms and 11 single-nucleotide polymorphisms related to burning pain and capsaicin sensitivity, respectively. In particular, homozygous I585V, a single-nucleotide polymorphism with amino acid substitution, significantly related to higher capsaicin sensitivity.


Assuntos
Capsaicina/efeitos adversos , Hiperalgesia/induzido quimicamente , Dor/genética , Polimorfismo de Nucleotídeo Único/genética , Fármacos do Sistema Sensorial/efeitos adversos , Canais de Cátion TRPV/genética , Adulto , Povo Asiático/genética , Mapeamento Cromossômico , Feminino , Humanos , Masculino , Medição da Dor , Estatísticas não Paramétricas , Adulto Jovem
3.
Anat Rec (Hoboken) ; 299(7): 929-42, 2016 07.
Artigo em Inglês | MEDLINE | ID: mdl-26916909

RESUMO

Previous studies have found a few intralingual ganglionic cells that were immunoreactive to vasoactive intestinal polypeptide (VIP) in the frog. A recent study reported a large number of such cells, and the possibility of the release of substance P (SP) from these. The aim of the present study was to investigate the distribution, origin, and colocalization of VIP- and SP- immunoreactive nerves in the tongue of the bullfrog, R. catesbeiana. In addition, the study also examined the colocalization of SP and phospholipase Cß2 (PLCß2 ) in the tongue and jugular ganglion. VIP immunoreactivity was seen in unipolar cells that were sparse in nerve bundles in the submucosal and muscle layers. The density of VIP-immunoreactive cells was approximately 4.8 cells/mm(3) . Their fibers terminated in the vicinity of the epithelial basal layer of the fungiform papillae. SP immunoreactivity was not seen in the VIP-immunoreactive cells, but was observed in pseudounipolar cells in the jugular ganglion. The SP fibers terminated close to the free surface, showing spindle- and button-like profiles. Transection of glossopharyngeal nerve resulted in the persistence of VIP-immunoreactive cells and the disappearance of SP-immunoreactive fibers in the tongue. SP immunoreactivity was co-expressed with PLCß2 in both the tongue and jugular ganglia. No PLCß2 immunoreactivity was seen in cells comprising the epithelial taste disk. These findings indicate that the origin of VIP nerve fibers are unipolar cells in the tongue, and SP and PLCß2 fibers originate from pseudounipolar cells that may be able to release SP primarily in the jugular ganglion. Anat Rec, 299:929-942, 2016. © 2016 Wiley Periodicals, Inc.


Assuntos
Gânglios/imunologia , Fibras Nervosas/imunologia , Fosfolipase C beta/metabolismo , Substância P/metabolismo , Língua/imunologia , Peptídeo Intestinal Vasoativo/metabolismo , Animais , Biomarcadores/metabolismo , Feminino , Gânglios/metabolismo , Técnicas Imunoenzimáticas , Masculino , Fibras Nervosas/metabolismo , Rana catesbeiana , Língua/metabolismo
4.
Anim Sci J ; 84(9): 650-5, 2013 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-23607929

RESUMO

Three types of bamboo pellets as a ruminant feed: P1 (ground bamboo (GB) cultured with the fungus Ceriporiopsis subvermispora (CGB) : soybean curd residue (T) : soy sauce cake (S) in a 5:4:1 ratio on a dry matter (DM) basis); P2 (GB : T : S = 5:4:1 on a DM basis); and P3 (CGB : T : S = 5.5:0.8:3.7 on a DM basis) were prepared. Four wethers were assigned in a 4 × 4 Latin square design experiment to evaluate the applicability of the bamboo pellets. The experimental treatments were C (control): fed alfalfa hay cubes (AC) only, and T1, T2 and T3: fed P1, P2, and P3 with AC by 1:1 on a DM basis, respectively. The digestibility of the DM, organic matter and acid detergent fiber of P1 were significantly higher than those of P2 and P3 (P < 0.05). The total digestible nutrient (TDN) contents of AC, P1, P2 and P3 were 56.5%, 60.2%, 53.2% and 47.0%, respectively. No significant differences in nitrogen retention or ruminal pH and NH3 were observed among the treatment groups. The results indicate that bamboo pellets cultured with C. subvermispora and mainly mixed with soybean curd residue improved nutritional quality of ground bamboo because of its high digestibility and TDN content.


Assuntos
Ração Animal , Digestão/fisiologia , Ingestão de Alimentos/fisiologia , Nitrogênio/metabolismo , Ovinos/fisiologia , Animais , Coriolaceae , Masculino , Sasa , Ovinos/sangue , Alimentos de Soja
5.
Acta Odontol Scand ; 67(6): 388-92, 2009.
Artigo em Inglês | MEDLINE | ID: mdl-19707900

RESUMO

OBJECTIVE: The aim of the study was to investigate the ultrastructural features of Malassez epithelium (ME) containing protein gene product 9.5 (PGP9.5)-immunoreactive (IR) cells in the cat periodontal ligament (PDL). MATERIAL AND METHODS: Specimens from the teeth and tooth-supporting tissues of four mature cats of both sexes, 18 to 24 months of age, were used. The fixed jaws were decalcified in EDTA. Frozen sagittal sections 20 µm thick were immunostained for PGP9.5, and the ME, containing IR cells in the PDL, were evaluated under a transmission electron microscope. RESULTS: Several epithelial cells and PGP9.5 IR cells formed clusters and were enveloped by a basal lamina and separated from the surrounding connective tissue. A large nucleus and scanty cytoplasm were observed in most of the ME cells, which contained abundant keratin filaments and mitochondria. Caveolae-like structures and vesicles were found in the periphery of the ME. The small cytoplasmic processes of some of the epithelial cells extended toward the surrounding connective tissues. The cytoplasmic matrix of one type of cell comprising the ME showed immunoreactivity for anti-PGP9.5 antibody. The IR cell in the cell clusters was connected to adjacent epithelial cells and extended cytoplasmic processes toward the adjacent epithelial cells. The IR cell contained keratin filaments and abundant densely cored vesicles approximately 100-250 nm in diameter. CONCLUSIONS: The findings of the study suggest endocytotic capabilities of the epithelial cells and neuroendocrine functions of the IR cells. It is possible that the two different cell types react to extrinsic stimuli and interact with cells comprising the clusters and cords in the PDL. These ultrastructural evidences may imply functional heterogeneity of the ME in the PDL.


Assuntos
Ligamento Periodontal/enzimologia , Ubiquitina Tiolesterase/análise , Animais , Membrana Basal/enzimologia , Membrana Basal/ultraestrutura , Gatos , Núcleo Celular/enzimologia , Núcleo Celular/ultraestrutura , Células do Tecido Conjuntivo/enzimologia , Células do Tecido Conjuntivo/ultraestrutura , Citoplasma/enzimologia , Citoplasma/ultraestrutura , Estruturas Citoplasmáticas/ultraestrutura , Citoesqueleto/ultraestrutura , Cemento Dentário/citologia , Desmossomos/ultraestrutura , Células Epiteliais/enzimologia , Células Epiteliais/ultraestrutura , Feminino , Queratinas/análise , Masculino , Microscopia Eletrônica de Transmissão , Mitocôndrias/ultraestrutura , Membrana Nuclear/ultraestrutura , Ligamento Periodontal/citologia , Vacúolos/ultraestrutura
6.
Bone ; 45(5): 980-6, 2009 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-19631304

RESUMO

In the process of bone resorption, calcium is considered to be transported within vesicles in osteoclasts and eventually released. We studied the ultramicromorphology of calcium (Ca) transport in osteoclasts by preparing samples of osteoclasts collected from rat femurs in which calcium was maximally preserved and subjected them to high-pressure quick-freezing and freeze-substitution. We then examined the localization of calcium by Electron Energy Loss Spectroscopy (EELS). The structures of cell membranes were preserved, suggesting the suitability of this high-pressure quick-freezing and freeze-substitution technique. Osteoclast mitochondria adjacent to the ruffled border were rich in mitochondrial granules and contained a large amount of Ca. In contrast, mitochondria in the basolateral region contained few granules. Moreover, by an osteoclast-culturing experiment, differences in the morphology of mitochondrial granules were noted between culturing on a dentin slice and that on a gold plate, i.e., few mitochondrial granules were noted in osteoclasts cultured on a non-dentin plate. These findings suggest an association between the morphology of mitochondrial granules in osteoclasts and bone resorption as well as a new transport route for Ca resorbed by osteoclasts. We propose that Ca accumulates in mitochondria granules to prevent increased Ca concentration in cytoplasm of osteoclasts during bone resorption.


Assuntos
Reabsorção Óssea/metabolismo , Cálcio/metabolismo , Mitocôndrias/metabolismo , Osteoclastos/metabolismo , Animais , Matriz Óssea/ultraestrutura , Células Cultivadas , Substituição ao Congelamento , Congelamento , Masculino , Camundongos , Mitocôndrias/ultraestrutura , Osteoclastos/ultraestrutura , Pressão , Ratos , Ratos Wistar , Análise Espectral
7.
J Histochem Cytochem ; 57(4): 315-25, 2009 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-19029402

RESUMO

This study sought to investigate the distribution of cytokeratin (CK)-immunopositive cells and their relationship to immunocompetent ED1- and OX6-immunopositive cells in rat periodontium by immunohistochemistry and electron microscopy. CK-immunopositive cells were generally distributed along the surface of the tooth root. They could also be found between root dentin and cementum, in the perivascular space, and close to or in the alveolar bone lacunae. ED1-immunopositive cells exhibited a compact shape with small processes and were widely distributed in the periodontium. Few sections demonstrated an intimate relationship between the CK- and ED1-immunopositive cells close to the cementum, in the perivascular space, and close to or in the alveolar bone. Numerous OX6-immunopositive cells with long branching processes were widely distributed in the periodontal ligament, surrounding and holding CK-immunopositive cells in the cell clusters, close to the cementum. Transmission electron microscopy revealed OX6-immunopositive cells that extended their cytoplasmic processes, which contained vesicles and occasionally lysosomes in between the epithelial cells. This study demonstrates the close relationship between the epithelial cells and the immunocompetent cells in a rat periodontium, indicating a functional interrelationship. It is possible that in a non-inflammatory periodontium, the epithelial cells act not independently, but through interaction with immunocompetent cells.


Assuntos
Ectodisplasinas/metabolismo , Células Epiteliais/citologia , Antígenos de Histocompatibilidade Classe II/metabolismo , Queratinas/metabolismo , Dente Molar/citologia , Dente Molar/metabolismo , Animais , Anticorpos Monoclonais , Ectodisplasinas/imunologia , Antígenos de Histocompatibilidade Classe II/imunologia , Imuno-Histoquímica , Queratinas/imunologia , Masculino , Maxila , Microscopia Confocal , Microscopia Eletrônica de Transmissão , Microscopia Imunoeletrônica , Periodonto/metabolismo , Ratos , Ratos Wistar
8.
Anat Rec (Hoboken) ; 291(3): 242-53, 2008 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-18231977

RESUMO

It has been shown that human and cat epithelial cell rests of Malassez (ERM) consist of heterogeneous cell populations. Immunohistochemical and immunoelectron microscopic analyses have verified the presence of neuroendocrine and Merkel-like cells in both of these epithelia. During experimental orthodontic tooth movement, immunocompetent cells have also been found in the vicinity of ERM in rat periodontal ligament (PDL), but have not been characterized in normal rat PDL. The aim of this study was to investigate the presence and distribution of MHC class II antigen presenting cells by using OX6 antibody in ERM of rat molars by light and transmission electron microscopy. Immunohistochemical and immunoelectron microscopic observations of rat maxillary molars confirmed the presence of OX6-positive cells in contact with ERM. Some immunopositive cytoplasmic processes containing vesicles interdigitated with cells of the Malassez epithelial clusters. Based on these findings it can be concluded that immunocompetent cells are localized close to Malassez epithelial clusters in normal rat PDL. Furthermore, the ultrastructural evidences indicate a possible interaction between the epithelial and immunocompent cells and suggest morphological and functional properties for ERM.


Assuntos
Células Apresentadoras de Antígenos , Células Epiteliais , Antígenos de Histocompatibilidade Classe II/análise , Imuno-Histoquímica , Microscopia Eletrônica de Transmissão , Microscopia Imunoeletrônica , Dente Molar/citologia , Ligamento Periodontal/citologia , Animais , Anticorpos Monoclonais , Células Apresentadoras de Antígenos/imunologia , Células Apresentadoras de Antígenos/ultraestrutura , Comunicação Celular , Vesículas Citoplasmáticas/imunologia , Vesículas Citoplasmáticas/ultraestrutura , Células Epiteliais/imunologia , Células Epiteliais/ultraestrutura , Masculino , Dente Molar/imunologia , Dente Molar/ultraestrutura , Ligamento Periodontal/imunologia , Ligamento Periodontal/ultraestrutura , Ratos , Ratos Wistar
9.
Okajimas Folia Anat Jpn ; 85(3): 97-101, 2008 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-19227200

RESUMO

A divided maxillary artery was found in the left infratemporal fossa of a 63-year-old male cadaver during a student dissection practice. The maxillary artery, which arose from the external carotid artery at the posterior border of the ramus of the mandible, was divided into two branches in the infratemporal fossa. The deep branch of the maxillary artery was deep to the lateral pterygoid muscle and the inferior alveolar, buccal and lingual nerves, giving off the anterior tympanic, middle meningeal, anterior deep temporal, buccal, and posterior superior alveolar arteries. The deep branch passed through the pterygomaxillary fissure. On the other hand, the superficial branch of the maxillary artery, which was superficial to the lateral pterygoid muscle, gave off the inferior alveolar artery and other branches. The maxillary artery on the right side displayed a normal branching pattern within the infratemporal fossa. These findings indicate that the deep and superficial branches of the maxillary artery remained without disappearing during the development of the maxillary artery originated from the stapedial artery.


Assuntos
Artéria Carótida Externa/anormalidades , Cabeça/irrigação sanguínea , Artéria Maxilar/anormalidades , Cadáver , Artéria Carótida Externa/fisiologia , Dissecação , Cabeça/fisiologia , Humanos , Arcada Osseodentária/irrigação sanguínea , Arcada Osseodentária/fisiologia , Masculino , Mandíbula/irrigação sanguínea , Mandíbula/fisiologia , Músculos da Mastigação/irrigação sanguínea , Músculos da Mastigação/fisiologia , Artéria Maxilar/fisiologia , Pessoa de Meia-Idade , Nervo Trigêmeo/anatomia & histologia
10.
Forensic Sci Int ; 149(2-3): 129-32, 2005 May 10.
Artigo em Inglês | MEDLINE | ID: mdl-15749352

RESUMO

Identification using lip prints was first performed in the 1950s and was the subject of much research in the 1960s and 70s, leading to the acceptance of this technique as evidence in the criminal justice system. Previous research has focused on identifying lip print types or on methods of obtaining hidden lip prints left at the crime scene. The present study aimed to clarify characteristics of lip prints from cadavers with various causes of death (including drowning and hanging) and to determine the effects of fixation on post mortem changes in lip impressions.


Assuntos
Medicina Legal/métodos , Lábio/patologia , Mudanças Depois da Morte , Adulto , Idoso , Idoso de 80 Anos ou mais , Cadáver , Feminino , Humanos , Masculino , Pessoa de Meia-Idade
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