RESUMO
OBJECTIVE: To investigate the effects of Vero cell co-culture on the development of mouse embryo in vitro and the expression of bax and bcl-2 genes in the mouse embryo. METHODS: The 2-cell mouse embryos were obtained from oviduct of 5-6 weeks old mated female ICR mice superovulated with pregnant mare's serum gonadotropin (PMSG) and human chorionic gonadotropin (hCG). The 2-cell embryos in Ham's F-10 medium supplemented with 10% FBS with or without Vero cells, were incubated at 37 degrees C in a 5% CO2 humidified air chamber respectively and we observed mouse embryo development and collected each group of embryos for the purpose of extraction of total RNA every 24 hours for 3 days. We carried out the RT-PCR to assess mRNA levels for bax and bcl-2 gene. RESULTS: The rate of embryo development of with Vero cell co-cultured group was 78.3%, 50.7%, 27.2% and that of without Vero cell co- cultured group was 60.5%, 29.3%, 20.7% respectively. Bax mRNA expression level of without Vero cell co-cultured group was significantly higher than that of with Vero cell co-cultured group at 24 hours. Bcl-2 mRNA expression level of Vero cell co-cultured group was significantly higher than that of without Vero cell co-cultured group at 72 hours. CONCLUSION: These findings suggested that Vero cell co-culture is beneficial in the development of mouse embryos and stimulates bax and bcl-2 gene expression.
Assuntos
Animais , Feminino , Humanos , Camundongos , Gravidez , Gonadotropina Coriônica , Técnicas de Cocultura , Desenvolvimento Embrionário , Estruturas Embrionárias , Genes bcl-2 , Gonadotropinas , Camundongos Endogâmicos ICR , Oviductos , RNA , RNA Mensageiro , Células VeroRESUMO
OBJECTIVE: To evaluate the plasma concentration of plasminogen activator inhibitor 1, main regulator of fibrinolytic system in women with polycystic ovary syndrome and to clarify whether it may be involved in the pathogenesis of chronic anovulation. METHODS: Fibrinolytic system (plasma fibrinogen, plasminogen, plasminogen activator inhibitor 1 concentration) was assayed in women with polycystic ovary syndrome and compared to normal controls. RESULTS: Women with polycystic ovary syndrome had significantly higher plasminogen activator inhibitor 1 and fibrinogen concentration compared to normal controls. CONCLUSION: Women with polycystic ovary syndrome may have an imbalance in the fibrinolytic system that is tilted towards a reduced production of the proteolytic enzyme plasmin. It may result in impaired follicular rupture and anovulation at cellular level in the ovaries.
Assuntos
Feminino , Humanos , Anovulação , Fibrinogênio , Fibrinolisina , Ovário , Plasma , Plasminogênio , Inibidor 1 de Ativador de Plasminogênio , Síndrome do Ovário Policístico , RupturaRESUMO
No abstract available.
