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1.
J Clin Biochem Nutr ; 73(1): 9-15, 2023 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-37534089

RESUMO

We used cold atmospheric pressure plasma jet to examine in detail 1O2 generation in water. ESR with 2,2,5,5-tetramethyl-3-pyrroline-3-carboxamide, a secondary amine probe, was used for the detection of 1O2. Nitroxide radical formation was detected after cold atmospheric pressure plasma jet irradiation of a 2,2,5,5-tetramethyl-3-pyrroline-3-carboxamide solution. An 1O2 scavenger/quencher inhibited the ESR signal intensity induced by cold atmospheric pressure plasma jet irradiation, but this inhibition was not 100%. As 2,2,5,5-tetramethyl-3-pyrroline-3-carboxamide reacts with oxidizing species other than 1O2, it was assumed that the signal intensity inhibited by NaN3 corresponds to only the nitroxide radical generated by 1O2. The concentration of 1O2 produced by cold atmospheric pressure plasma jet irradiation for 60 s was estimated at 8 µM. When this 1O2 generation was compared to methods of 1O2 generation like rose bengal photoirradiation and 4-methyl-1,4-etheno-2,3-benzodioxin-1(4H)-propanoic acid (endoperoxide) thermal decomposition, 1O2 generation was found to be, in decreasing order, rose bengal photoirradiation ≥ cold atmospheric pressure plasma jet > endoperoxide thermal decomposition. Cold atmospheric pressure plasma jet is presumed to not specifically generate 1O2, but can be used to mimic states of oxidative stress involving multiple ROS.

2.
Chem Pharm Bull (Tokyo) ; 69(1): 67-71, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33390522

RESUMO

We examined the method of oxidative hemolysis for assessment of antioxidant activity of various compounds, especially lipophilic compounds. 2,2'-Azobis(amidinopropane) dihydrochloride (AAPH) was used as the source of free radicals for the oxidative hemolysis of horse erythrocytes. We found that absorbance at 540 nm is not appropriate for monitoring AAPH-induced hemolysis. Instead, we should use absorbance at 523 nm (an isosbestic point), because AAPH oxidizes the oxygenated hemoglobin to methemoglobin and absorbance at 540 nm does not correctly reflect the amount of released hemoglobin by AAPH-induced hemolysis. The corrected method of AAPH-induced hemolysis was applicable to assess the antioxidant activity of various hydrophilic compounds such as ascorbic acid, (-)-epicatechin, and edaravone. For the assessment of antioxidant activity of lipophilic compounds, we need appropriate dispersing agents for these lipophilic compounds. Among several agents tested, 1,2-dimiristoyl-sn-glycero-3-phosphocholine (DMPC) liposome at a concentration of 0.34 mM was found to be useful. Exogenous α-tocopherol incorporated using DMPC liposome as a dispersing agent was shown to protect erythrocytes from AAPH-induced hemolysis in a concentration-dependent manner.


Assuntos
Antioxidantes/farmacologia , Compostos Azo/farmacologia , Eritrócitos/efeitos dos fármacos , Animais , Antioxidantes/química , Compostos Azo/química , Relação Dose-Resposta a Droga , Eritrócitos/metabolismo , Hemólise/efeitos dos fármacos , Cavalos , Interações Hidrofóbicas e Hidrofílicas
3.
Chem Pharm Bull (Tokyo) ; 68(2): 150-154, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32009082

RESUMO

Singlet oxygen (1O2) is highly oxidative and exerts strong cytotoxic effects. We tried to establish the best combination of a singlet oxygen generation system and a detection method with ESR, for measurement of the quenching activities of various substances. The photosensitizing reaction of rose bengal or thermal decomposition of 4-methyl-1,4-etheno-2,3-benzodioxin-1(4H)-propanoic acid (endoperoxide, EP) was used for the generation of 1O2, and a sterically hindered secondary amine, 2,2,6,6-tetramethyl-4-piperidone (TEMPD) or 2,2,6,6-tetramethyl-4-piperidinol (TEMP-OH), was used as the 1O2 detection probe. These secondary amines were oxidized by 1O2 to form stable nitroxide radicals, which were detectable by ESR. TEMPD was found to be readily oxidized by air, causing large background signals in comparison with TEMP-OH. The ESR signal obtained by the irradiation of rose bengal with visible light in the presence of TEMP-OH consisted of two kinds of nitroxide radical overlapping. In contrast, only a single nitroxide signal was observed when TEMP-OH was reacted with 1O2 generated from EP. Therefore, the best combination should be EP as the 1O2 generator and TEMP-OH as the detection probe. When using this combination, we found that the concentrations of some organic solvents such as dimethyl sulfoxide and acetonitrile should be kept constant for reliable quantification, because the concentrations of organic solvents affect the ESR signal intensity.


Assuntos
Espectroscopia de Ressonância de Spin Eletrônica/métodos , Oxigênio Singlete/análise , Oxirredução , Fármacos Fotossensibilizantes/química , Piperidonas/química , Propionatos/química , Rosa Bengala/química
4.
J Clin Biochem Nutr ; 64(3): 187-193, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-31138951

RESUMO

Because application of cold atmospheric pressure plasma jet (CAPPJ) to biological samples have taken large attentions, it is important to examine the effects of various CAPPJ parameters on the generation of reactive species. Here, we investigated the generation of reactive species in water by CAPPJ irradiation by changing the following parameters: irradiation time, sample volume, and irradiation distance between the sample surface and plasma jet tip. We measured 1) change in the ESR signal intensity of 4-hydroxy-2,2,6,6-tetrametylpeperidine-1-oxyl (Tempol), 2) spin-trapping with 5,5-dimethyl-1-pyrroline N-oxide (DMPO), 3) Fricke dosimeter reaction, and 4) hydrogen peroxide (H2O2) formation induced by CAPPJ irradiation. By the experiment of volume dependency, it is suggested that the reactive species detected in water are formed largely in the plasma gas phase. The reduction of ESR signal intensity of Tempol and the formation of DMPO-OH were strongly dependent on irradiation distance, but the relationship between H2O2 generation and distance was weak. The formation of species that oxidize Fe2+ to Fe3+ was shown by the Fricke dosimeter reaction, and reactions at irradiation distances longer than 3 cm were mainly attributable to H2O2. It may be possible to apply different reactive species to the samples by changing the CAPPJ irradiation distance.

5.
Oxid Med Cell Longev ; 2014: 901315, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-24723997

RESUMO

Toona sinensis is one of the most popular vegetarian cuisines in Taiwan and it has been shown to possess antioxidant, antiangiogenic, and anticancer properties. In this study, we investigated the antiatherosclerotic potential of aqueous leaf extracts from Toona sinensis (TS; 25-100 µg/mL) and its major bioactive compound, gallic acid (GA; 5 µg/mL), in LPS-treated rat aortic smooth muscle (A7r5) cells. We found that pretreatment with noncytotoxic concentrations of TS and GA significantly inhibited inflammatory NO and PGE2 production by downregulating their precursors, iNOS and COX-2, respectively, in LPS-treated A7r5 cells. Furthermore, TS and GA inhibited LPS-induced intracellular ROS and their corresponding mediator, p47(phox). Notably, TS and GA pretreatment significantly inhibited LPS-induced migration in transwell assays. Gelatin zymography and western blotting demonstrated that treatment with TS and GA suppressed the activity or expression of MMP-9, MMP-2, and t-PA. Additionally, TS and GA significantly inhibited LPS-induced VEGF, PDGF, and VCAM-1 expression. Further investigation revealed that the inhibition of iNOS/COX-2, MMPs, growth factors, and adhesion molecules was associated with the suppression of NF-κB activation and MAPK (ERK1/2, JNK1/2, and p38) phosphorylation. Thus, Toona sinensis may be useful for the prevention of atherosclerosis.


Assuntos
Movimento Celular/efeitos dos fármacos , Inflamação/tratamento farmacológico , Meliaceae/química , Músculo Liso Vascular/patologia , Miócitos de Músculo Liso/patologia , NF-kappa B/metabolismo , Extratos Vegetais/farmacologia , Espécies Reativas de Oxigênio/metabolismo , Animais , Linhagem Celular , Sobrevivência Celular/efeitos dos fármacos , Ciclo-Oxigenase 2/metabolismo , Dinoprostona/biossíntese , Regulação para Baixo/efeitos dos fármacos , Proteínas do Olho/metabolismo , Ácido Gálico/farmacologia , Proteínas I-kappa B/metabolismo , Lipopolissacarídeos , Sistema de Sinalização das MAP Quinases/efeitos dos fármacos , Miócitos de Músculo Liso/efeitos dos fármacos , Miócitos de Músculo Liso/enzimologia , NADPH Oxidases/metabolismo , Inibidor de NF-kappaB alfa , Fatores de Crescimento Neural/metabolismo , Óxido Nítrico/biossíntese , Óxido Nítrico Sintase Tipo II/metabolismo , Extratos Vegetais/uso terapêutico , Ratos , Serpinas/metabolismo , Transdução de Sinais/efeitos dos fármacos , Ativador de Plasminogênio Tecidual/metabolismo , Fator A de Crescimento do Endotélio Vascular/metabolismo
6.
Arch Biochem Biophys ; 516(2): 154-9, 2011 Dec 15.
Artigo em Inglês | MEDLINE | ID: mdl-22037355

RESUMO

Nitric oxide (()NO) is thought to react with fatty acid alkoxyl radical, which is generated from fatty acid hydroperoxide via one-electron reduction. However, detail in the reaction remains obscure. In the present study, we examined the reaction of nitric oxide with fatty acid alkoxyl radical generated in the lipoxygenase/linoleate/13-hydroperoxyoctadecadienoate (13-HpODE) system under anaerobic conditions via HPLC equipped with mass spectrometry and photodiode array detections. In this reaction system, nitric oxide can scavenge linoleate alkoxyl radical, producing 13-ONO-9Z,11E-ODE. However, instead of 13-ONO-9Z,11E-ODE, 13-NO-9E,11E-ODE and 9-NO-10E,12E-ODE were alternatively detected in the reaction solution. To explain this contradiction, we proposed a mechanism as follows: (1) 13-ONO-9E/11Z-ODE undergoes homolytic cleavage at >CHONO bond into the linoleate allyl radical and nitrogen dioxide, (2) the allyl radical undergoes resonance stabilization into the E/E-form, and (3) nitric oxide scavenges the E/E-pentadiene radical at C9 or C13 position. Consequently, we concluded that nitric oxide immediately converts fatty acid alkoxyl radical into allyl radical.


Assuntos
Ácidos Graxos/metabolismo , Óxido Nítrico/metabolismo , Álcoois/química , Álcoois/metabolismo , Araquidonato 15-Lipoxigenase/metabolismo , Cromatografia Líquida de Alta Pressão , Ácidos Graxos/química , Radicais Livres/química , Radicais Livres/metabolismo , Peroxidação de Lipídeos , Espectrometria de Massas , Modelos Biológicos , Óxido Nítrico/química , Oxirredução
7.
Biomed Chromatogr ; 24(7): 794-7, 2010 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-20082280

RESUMO

Nitroxyl radicals can trap fatty acid allyl radicals on ferric-lipoxygenases at lower oxygen content, which are an intermediate in the lipoxygenase reaction. In the present study, we examined whether nitroxyl radical-trapping of fatty acid allyl radicals on the enzyme proceeds in biological fluids with abundant antioxidants. The fatty acid allyl radical-nitroxyl radical adducts were quantified by HPLC with electrochemical detection (HPLC-ECD); the adducts in eluate degraded into nitroxyl radical by passing through heating coil at 100 degrees C, and then nitroxyl radical was detected by electrochemical detector. Soybean 15-lipoxygenase and nitroxyl radical (3-carbamoyl-2,2,5,5-tetramethyl-3-pyrroline-N-oxyl, CmDeltaP) were mixed with rat serum prepared from fresh venous blood, and the solution was stood at 37 degrees C for 1 h. One volume of the solution was mixed with 5 vols of cold acetonitrile. After centrifugation, the supernatant was subjected to HPLC-ECD. Arachidonate allyl radical-CmDeltaP adducts as well as linoleate allyl radical-CmDeltaP adducts were detected in the solution, and the content of these adducts remarkably increased in the presence of phospholipase A(2). It is proved for the first time that nitroxyl radical traps fatty acid allyl radicals generated in the lipoxygenase reaction in biological fluid without competition from endogenous antioxidants.


Assuntos
Araquidonato 15-Lipoxigenase/metabolismo , Cromatografia Líquida de Alta Pressão/métodos , Radicais Livres/análise , Óxidos de Nitrogênio/análise , Proteínas de Soja/metabolismo , Detecção de Spin/métodos , Animais , Cromatografia Líquida de Alta Pressão/instrumentação , Radicais Livres/sangue , Radicais Livres/metabolismo , Óxidos de Nitrogênio/sangue , Óxidos de Nitrogênio/metabolismo , Ratos , Detecção de Spin/instrumentação
8.
J Lipid Res ; 48(6): 1371-7, 2007 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-17337722

RESUMO

Ferrous lipoxygenases seem to be activated through a feedback control mechanism via FA hydroperoxides generated from PUFAs by partially existing ferric lipoxygenases. However, during leukotriene synthesis, feedback activation of ferrous 5-lipoxygenase in the presence of arachidonic acid (AA) was not observed. In the present study, we examined the feedback activation of ferrous 5-lipoxygenase in the 5-lipoxygenase/AA system in the presence of linoleic aicd (LA), which is a predominant component of membrane phospholipids. When potato 5-lipoxygenase was incubated with AA and LA in the presence of nitroxyl radical, 3-carbamoyl-2,2,5,5-tetramethyl-3-pyrroline-N-oxyl (CmDeltaP), one-electron redox cycle reaction between ferric and ferrous 5-lipoxygenase was detected. For each revolution of the cycle, one molecule of PUFA and one molecule of its hydroperoxide were converted into PUFA-allyl radical-CmDeltaP adduct ([PUFA-H].-CmDeltaP) and PUFA-epoxyallyl radical-CmDeltaP adduct ([PUFA-H+O].-CmDeltaP), respectively. The ratios, [AA-H].-CmDeltaP/[LA-H].-CmDeltaP and [AA-H+O].-CmDeltaP/[LA-H+O].-CmDeltaP, were estimated to be 1.7 and 0.13, respectively. These facts indicate that ferrous 5-lipoxygenase is activated through feedback control in the presence of LA, and that resulting ferric 5-lipoxygenase catalyzes the stoichiometric synthesis of leukotrienes from AA. In conclusion, the biosynthesis of leukotrienes is remarkably efficient.


Assuntos
Leucotrienos/química , Ácido Linoleico/química , Lipoxigenase/metabolismo , Ácido Araquidônico/química , Ácido Araquidônico/metabolismo , Cromatografia Líquida de Alta Pressão , Leucotrienos/metabolismo , Ácido Linoleico/metabolismo , Modelos Químicos , Óxidos de Nitrogênio/química
9.
Nitric Oxide ; 16(3): 356-61, 2007 May.
Artigo em Inglês | MEDLINE | ID: mdl-17293136

RESUMO

Nitric oxide (NO) is a possible agent, which induces crosslinking among molecules containing sulfhydryl groups. However, the S-thiolation is essentially accompanied by S-nitrosylation. In the present study, we evaluated radical scavengers as a regulator for S-thiolation and S-nitrosylation by NO released from NO-generator, 1-hydroxy-2-oxo-3-(N-methyl-3-aminopropyl)-3-methyl-1-triazene (P-NONOate). When glutathione was incubated with P-NONOate in 4% (vol/vol) O(2)-saturated buffer solution (pH 7.4) in the presence of nitrone spin-trapping agent, 5,5'-dimethyl-1-pyroline-N-oxide (DMPO), the prevention of S-thiolation and the promotion of S-nitrosylation were observed. The DMPO adduct was identified to be thiyl radical-DMPO adduct via ESR study. In contrast, nitroxyl radical, radical scavenger against oxygen-centered radicals, promoted the S-thiolation but prevented S-nitrosylation. Nitronyl nitroxide, radical scavenger against nitric oxide, can convert nitric oxide into nitrogen dioxide in the O(2)-independent manner. In the presence of nitronyl nitroxide in the thiol/P-NONOate system, S-thiolation was remarkably enhanced up to 60% (mol/mol) of sulfhydryl groups. However, nitronyl nitroxide at enough content (>or=1.0 mM) almost completely prevented S-nitrosylation, whereas nitronyl nitroxide at comparatively lower content (0.5 mM) contrarily enhanced the S-nitrosylation. Based on these facts, it appeared to be possible to consequently regulate S-thiolation and S-nitrosylation through controlling the thiyl radical chain reaction by radical scavengers.


Assuntos
Sequestradores de Radicais Livres/química , Óxido Nítrico/química , Compostos Nitrosos/química , Compostos de Sulfidrila/química , Enxofre/química , Antioxidantes/química , Óxidos N-Cíclicos/química , Radicais Livres/química , Glutationa/química , Estrutura Molecular , Oxigênio/química , Marcadores de Spin , Triazenos/química
10.
J Lipid Res ; 48(4): 855-62, 2007 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-17227895

RESUMO

Variation of tissue oxygen content is thought to be a possible factor in determining the structural diversity of hydroperoxy fatty acids. In the present study, we evaluated the structural diversity of intermediate carbon-centered radicals at lower oxygen content. When the buffered solution (pH 7.4) containing 1.0 mM alpha-linolenic acid, 1.0 muM soybean 15-lipoxygenase, and 1.0 mM nitroxyl radical [3-carbamoyl-2,2,5,5-tetramethyl-3-pyrroline-N-oxyl (CmDeltaP)], which selectively traps carbon-centered radicals, was incubated in a sealed vial, the generation of linolenate hydroperoxide was completed within 1 min. In the subsequent reaction at lower oxygen content, the production of the [LnA-H+O(2)].-CmDeltaP adduct was ascertained by liquid chromatography tandem mass spectrometry with precursor ion scanning. Furthermore, HPLC analysis with photodiode array detection showed that the adduct exhibits an absorption maximum at 278 nm, indicating a conjugated triene moiety. On the basis of these facts, the structure of the adduct was speculated to be C(2)H(5)-CH(CmDeltaP)-CH = CH-CH = CH-CH = CH-CH(OOH) -C(7)H(14)-COOH. We proposed a possible reaction pathway as follows: a linolenate 9-peroxyl radical generated in the lipoxygenase reaction might be converted into C(2)H(5)-.CH-CH = CH-CH = CH-CH = CH-CH(OOH) -C(7)H(14)-COOH through an intramolecular rearrangement. This intermediate radical may give rise to hydroperoxy fatty acids with structural diversity.


Assuntos
Araquidonato 15-Lipoxigenase/metabolismo , Radicais Livres/química , Oxigênio/farmacologia , Ácido alfa-Linolênico/metabolismo , Cromatografia Líquida de Alta Pressão , Ácidos Graxos/química , Espectrometria de Massas , Peróxidos
11.
Biochem J ; 395(2): 303-9, 2006 Apr 15.
Artigo em Inglês | MEDLINE | ID: mdl-16396633

RESUMO

Lipoxygenases have been proposed to be a possible factor that is responsible for the pathology of certain diseases, including ischaemic injury. In the peroxidation process of linoleic acid by lipoxygenase, the E,Z-linoleate allyl radical-lipoxygenase complex seems to be generated as an intermediate. In the present study, we evaluated whether E,Z-linoleate allyl radicals on the enzyme are scavenged by radical scavengers. Linoleic acid, the content of which was greater than the dissolved oxygen content, was treated with soya bean lipoxygenase-1 (ferric form) in the presence of radical scavenger, CmP (3-carbamoyl-2,2,5,5-tetramethylpyrrolidine-N-oxyl). The reaction rate between oxygen and lipid allyl radical is comparatively faster than that between CmP and lipid allyl radical. Therefore a reaction between linoleate allyl radical and CmP was not observed while the dioxygenation of linoleic acid was ongoing. After the dissolved oxygen was depleted, CmP stoichiometrically trapped linoleate-allyl radicals. Accompanied by this one-electron redox reaction, the resulting ferrous lipoxygenase was re-oxidized to the ferric form by hydroperoxylinoleate. Through the adduct assay via LC (liquid chromatography)-MS/MS (tandem MS), four E,Z-linoleate allyl radical-CmP adducts corresponding to regio- and diastereo-isomers were detected in the linoleate/lipoxygenase system, whereas E,E-linoleate allyl radical-CmP adducts were not detected at all. If E,Z-linoleate allyl radical is liberated from the enzyme, the E/Z-isomer has to reach equilibrium with the thermodynamically favoured E/E-isomer. These data suggested that the E,Z-linoleate allyl radicals were not liberated from the active site of lipoxygenase before being trapped by CmP. Consequently, we concluded that the lipid allyl radicals complexed with lipoxygenase could be scavenged by radical scavengers at lower oxygen content.


Assuntos
Sequestradores de Radicais Livres/metabolismo , Radicais Livres/metabolismo , Metabolismo dos Lipídeos , Lipoxigenase/metabolismo , Oxigênio/metabolismo , Ácido Araquidônico/metabolismo , Cromatografia Líquida de Alta Pressão , Cromatografia Líquida , Óxidos N-Cíclicos/metabolismo , Ácido Linoleico/metabolismo , Ácidos Linolênicos/metabolismo , Espectrometria de Massas , Óxidos de Nitrogênio/metabolismo , Glycine max , Detecção de Spin , Estereoisomerismo
12.
J Lipid Res ; 46(11): 2506-13, 2005 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-16106053

RESUMO

Lipid alkyl radicals generated from polyunsaturated fatty acids via chemical or enzymatic H-abstraction have been a pathologically important target to quantify. In the present study, we established a novel method for the quantification of lipid alkyl radicals via nitroxyl radical spin-trapping. These labile lipid alkyl radicals were converted into nitroxyl radical-lipid alkyl radical adducts using 3-carbamoyl-2,2,5,5-tetramethyl-3-pyrroline-N-oxyl (CmdeltaP) (a partition coefficient between octanol and water is approximately 3) as a spin-trapping agent. The resulting CmdeltaP-lipid alkyl radical adducts were determined by HPLC with postcolumn online thermal decomposition, in which the adducts were degraded into nitroxyl radicals by heating at 100 degrees C for 2 min. The resulting nitroxyl radicals were selectively and sensitively detected by electrochemical detection. With the present method, we, for the first time, determined the lipid alkyl radicals generated from linoleic acid, linolenic acid, and arachidonic acid via soybean lipoxygenase-1 or the radical initiator 2,2'-azobis(2,4-dimethyl-valeronitrile).


Assuntos
Bioquímica/instrumentação , Cromatografia Líquida de Alta Pressão/métodos , Radicais Livres/química , Lipídeos/química , Ácido Araquidônico/química , Compostos Azo/química , Bioquímica/métodos , Cromatografia , Cromatografia Líquida de Alta Pressão/instrumentação , Cromatografia Líquida , Óxidos N-Cíclicos/farmacologia , Eletroquímica , Temperatura Alta , Ácido Linoleico/química , Lipoxigenase/metabolismo , Espectrometria de Massas , Modelos Químicos , Nitrilas/química , Óxidos de Nitrogênio , Octanóis/química , Sensibilidade e Especificidade , Marcadores de Spin , Detecção de Spin , Temperatura , Fatores de Tempo , Água/química
13.
J Invest Dermatol ; 122(6): 1463-70, 2004 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-15175038

RESUMO

Although oxygen radicals are thought to play a key role in the skin injury that is caused by protoporphyria, there is no direct evidence of generation of these radicals in vivo. This study measured the generation of oxygen radicals caused by visible light non-invasively in the skin of griseofulvin-induced protoporphyria model mice, using an in vivo electron spin resonance spectrometer equipped with a surface-coil-type resonator that could detect radicals within about 0.5 mm of the skin surface. A durable nitroxyl radical was administered intravenously as a probe. Light irradiation enhanced the decay of the nitroxyl signal in griseofulvin-treated mice, whereas light irradiation did not enhance the signal decay in control mice. The enhanced signal decay was completely suppressed by intravenous administration of hydroxyl radical scavengers, superoxide dismutase or catalase, or the intraperitoneal administration of desferrioxamine. The enhanced signal decay with illumination was reversible, and quickly responded to turning the light on and off. These observations suggest that the hydroxyl radical is generated via an iron-catalyzed reaction in the skin. This paper demonstrates, for the first time, the specific generation of oxygen radicals in response to light irradiation of the skin of protoporphyria model mice.


Assuntos
Luz/efeitos adversos , Porfiria Hepatoeritropoética/metabolismo , Espécies Reativas de Oxigênio/metabolismo , Pele/metabolismo , Pele/efeitos da radiação , Animais , Antifúngicos , Catalase/farmacologia , Desferroxamina/farmacologia , Modelos Animais de Doenças , Espectroscopia de Ressonância de Spin Eletrônica , Griseofulvina , Radical Hidroxila/metabolismo , Quelantes de Ferro/farmacologia , Masculino , Camundongos , Porfiria Hepatoeritropoética/induzido quimicamente , Porfiria Hepatoeritropoética/tratamento farmacológico , Superóxido Dismutase/farmacologia
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