RESUMO
To open up a 1-microm waveband for photonic transport systems, we developed a hybrid and harmonically mode-locked semiconductor laser (MLL) that can transmit return-to-zero (RZ) optical signals at data rates on the order of gigabits per second. A single-mode hole-assisted fiber (HAF) was also developed for use as a 1-microm waveband signal transmission line. A stable optical pulse train with a repetition rate of 9.953 GHz, pulse width of 22 ps, and low timing jitter of 120 fs was obtained from a 1035-nm harmonically MLL. With these devices, we successfully demonstrated 1-microm waveband error-free transmission of a high-speed 10-Gbps RZ signal over a long distance of 7 km.
RESUMO
We developed a novel scheme for two-photon fluorescence bioimaging. We generated supercontinuum (SC) light at wavelengths of 600 to 1200 nm with 774-nm light pulses from a compact turn-key semiconductor laser picosecond light pulse source that we developed. The supercontinuum light was sliced at around 1030- and 920-nm wavelengths and was amplified to kW-peak-power level using laboratory-made low-nonlinear-effects optical fiber amplifiers. We successfully demonstrated two-photon fluorescence bioimaging of mouse brain neurons containing green fluorescent protein (GFP).
Assuntos
Encéfalo/citologia , Aumento da Imagem/instrumentação , Lasers , Microscopia de Fluorescência por Excitação Multifotônica/instrumentação , Neurônios/citologia , Animais , Desenho de Equipamento , Análise de Falha de Equipamento , Aumento da Imagem/métodos , Camundongos , Microscopia de Fluorescência por Excitação Multifotônica/métodos , SemicondutoresRESUMO
Toward a practical light source for two-photon bioimaging, we have generated kilowatt peak power of 0.77 mum wavelength and 5 ps optical pulse via second-harmonic generation of the amplified output from a gain-switched 1.55 mum semiconductor laser. This compact scheme and stable optical-pulse-source has been successfully used for the two-photon fluorescence bioimaging of actin filaments in PtK2 cells.
