RESUMO
Although rubella is epidemic in Indonesia, the phylogenetic profile of circulating rubella virus strains has not been clarified. In 2017, rubella virus was detected in 2 travelers who returned from Indonesia to Japan. These strains were classified into genotype 1E lineage 2, which may be an indigenous strain in Indonesia.
Assuntos
Vírus da Rubéola/isolamento & purificação , Rubéola (Sarampo Alemão)/diagnóstico , Viagem , Adulto , Diagnóstico Diferencial , Genótipo , Humanos , Indonésia , Japão , Masculino , Filogenia , Rubéola (Sarampo Alemão)/prevenção & controle , Rubéola (Sarampo Alemão)/virologia , Vírus da Rubéola/classificação , Vírus da Rubéola/genéticaRESUMO
The synthesis and structural analysis of the hetero-bimetallic M-Al complexes MMe[(O-2,4-(t)Bu2C6H2-6-CH2)2(µ2-O-2,4-(t)Bu2C6H2-6-CH2)N][Me2Al(µ2-O(i)Pr)] [M = Zr, Hf], prepared from M(O(i)Pr)[{(O-2,4-(t)Bu2C6H2)-6-CH2}3N] by treating with 1.0 equiv. of AlMe3, have been explored: these complexes exhibited catalytic activities for ethylene polymerisation in n-octane in the presence of methylaluminoxane (MAO). The Zr-Al analogue showed high catalytic activities affording polymers with unimodal molecular weight distributions.
RESUMO
We have developed a new DNA chip whose substrate has a unique minute columnar array structure made of plastic. The DNA chip exhibits ultrahigh sensitivity, up to 100-fold higher than that of reference DNA chips, which makes it possible to monitor gene expression profiles even with very small amounts of RNA (0.1-0.01 microg of total RNA) without amplification. Differential expression ratios obtained with the new DNA chip were validated against those obtained with quantitative real-time PCR assays. This novel microarray technology would be a powerful tool for monitoring gene expression profiles, especially for clinical diagnosis.
Assuntos
Perfilação da Expressão Gênica/métodos , Técnicas de Amplificação de Ácido Nucleico/métodos , Análise de Sequência com Séries de Oligonucleotídeos/métodos , RNA/metabolismo , Perfilação da Expressão Gênica/instrumentação , Microscopia Eletrônica de Varredura , Hibridização de Ácido Nucleico/métodos , Análise de Sequência com Séries de Oligonucleotídeos/instrumentação , Ativadores de Plasminogênio/genética , RNA/genética , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Reprodutibilidade dos Testes , Reação em Cadeia da Polimerase Via Transcriptase Reversa/métodos , Estatísticas não ParamétricasRESUMO
The respiratory activity of collagen-embedded living cells was imaged by scanning electrochemical microscopy (SECM) with the objective to study anticancer drug sensitivity. Two kinds of cancer cells, the human erythroleukemia cell line (K562) and its adriamycin-resistant subline (K562/ADM), were immobilized at the array of microholes micromachined on a silicon wafer for comparative characterization of their sensitivity to the anticancer drug, ADM. The results obtained by the SECM method showed correspondence to a conventional colorimetric assay (SDI assay). Furthermore, since the SECM assay is based on the noninvasive measurement of the respiration activity, continuous monitoring of a dose response was possible.
